Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GKC70_RS22515 Genome accession   NZ_CP046139
Coordinates   4990050..4991276 (-) Length   408 a.a.
NCBI ID   WP_317870991.1    Uniprot ID   -
Organism   Pseudomonas sp. REB1044     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4985050..4996276
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKC70_RS22500 (GKC70_22255) - 4986694..4988319 (-) 1626 WP_342649977.1 AAA family ATPase -
  GKC70_RS22505 (GKC70_22260) aroB 4988316..4989431 (-) 1116 WP_342649978.1 3-dehydroquinate synthase -
  GKC70_RS22510 (GKC70_22265) aroK 4989527..4990045 (-) 519 WP_039582580.1 shikimate kinase AroK -
  GKC70_RS22515 (GKC70_22270) pilQ 4990050..4991276 (-) 1227 WP_317870991.1 secretin N-terminal domain-containing protein Machinery gene
  GKC70_RS22520 (GKC70_22275) - 4991242..4992180 (-) 939 WP_342649979.1 pilus assembly protein PilP -
  GKC70_RS22525 (GKC70_22280) - 4992264..4992797 (-) 534 WP_342649980.1 PilN domain-containing protein -
  GKC70_RS22530 (GKC70_22285) pilM 4992794..4993723 (-) 930 WP_342649981.1 pilus assembly protein PilM -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 43741.88 Da        Isoelectric Point: 5.1684

>NTDB_id=401586 GKC70_RS22515 WP_317870991.1 4990050..4991276(-) (pilQ) [Pseudomonas sp. REB1044]
MQALATTAYKGEPLSLNFQDVEVRAVLQVLADYAGVNLVASDTVQGNVTLRLQDVPWDQALDLVLRSRGLARREQGNVLL
IAPAAELAEQTRETQADRLLEARSQPLQRALLPVHHADASALAPLLLASLALDDPADARATLSVDPRTNTLVAVQSAARL
AELRQLIAQLDVPVRQVMIEARIVEANVDYEKVLGVRWGAKHGGSPGRSGSELFVDLGASAATSGIGLGLVGSDALLDLQ
LSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQQSSKSGATSVSFREASLSLEVTPQITPDGKVIMAVRVTKDEPD
YANALNDVPPIRKNEVNAKVRVADGQTIVIGGVYSTSNSNVVDKVPFLGDLPYVGRLFRRDALQEKKSELLVFLTPRIMN
DQAIAVSR

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=401586 GKC70_RS22515 WP_317870991.1 4990050..4991276(-) (pilQ) [Pseudomonas sp. REB1044]
ATGCAGGCACTGGCGACGACGGCCTACAAAGGTGAGCCGCTGTCGCTGAACTTCCAGGATGTGGAAGTGCGAGCGGTGCT
GCAGGTGCTGGCCGACTATGCAGGCGTCAATCTGGTGGCCAGCGACACGGTCCAGGGCAACGTTACGCTGCGCTTGCAGG
ACGTACCCTGGGACCAGGCGCTGGACCTGGTGCTGCGCAGTCGCGGATTGGCCAGGCGCGAGCAGGGTAATGTACTGCTG
ATCGCCCCTGCCGCCGAGCTTGCCGAGCAAACGCGCGAGACGCAGGCTGACCGCCTGCTGGAGGCGCGGTCGCAGCCGCT
GCAGCGCGCGCTGCTGCCGGTTCACCATGCCGATGCGTCCGCGCTGGCACCGCTGTTGCTGGCCAGCCTGGCCCTCGACG
ACCCGGCGGATGCGCGCGCCACGCTGAGCGTGGATCCACGCACCAACACCCTCGTCGCCGTCCAGTCCGCCGCGCGGCTG
GCCGAACTGCGGCAGTTGATCGCGCAGTTGGACGTGCCGGTGCGTCAGGTGATGATCGAGGCGCGCATCGTCGAGGCCAA
CGTCGACTACGAGAAGGTGCTCGGCGTGCGCTGGGGCGCCAAACATGGCGGTTCGCCGGGACGCTCGGGCAGCGAACTGT
TCGTCGATCTCGGCGCCTCGGCTGCCACGTCCGGCATCGGTCTAGGCTTGGTGGGCAGCGATGCGCTGCTCGACTTGCAG
CTCAGCGCCATGGAAAAGAGCGGGAATGGCGAGATCATCTCGCAGCCCAAAGTGGTCACGGCCGACAAGGAAACCGCACG
CATCCTCAAGGGCACCGAGGTGCCGTACCAGCAGTCGAGCAAGAGTGGCGCCACCTCGGTGTCGTTCCGCGAGGCGTCGC
TTTCATTGGAGGTCACCCCGCAAATCACCCCTGACGGCAAAGTCATCATGGCGGTGCGCGTGACCAAGGACGAGCCCGAC
TACGCCAATGCCCTCAACGACGTGCCGCCGATTCGCAAGAACGAGGTCAACGCCAAGGTGCGCGTGGCCGACGGCCAGAC
CATTGTGATTGGTGGCGTGTATTCGACGTCGAACAGCAATGTGGTCGACAAGGTGCCATTTCTTGGCGATCTGCCGTATG
TTGGACGGCTTTTTCGACGCGATGCATTACAAGAGAAAAAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAAT
GACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(111-176)

Bacterial type II and III secretion system protein (Secretin)

(243-399)

Secretin and TonB N terminus short domain (STN)

(37-83)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

58.691

100

0.637
  pilQ Acinetobacter baumannii D1279779

41.649

100

0.471
  pilQ Acinetobacter baumannii strain A118

41.649

100

0.471
  comQ Acinetobacter baylyi ADP1

42.667

100

0.471
  pilQ Legionella pneumophila strain ERS1305867

41.08

100

0.429
  pilQ Legionella pneumophila strain Lp02

41.08

100

0.429
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.527

100

0.426
  pilQ Vibrio cholerae strain A1552

41.527

100

0.426
  pilQ Vibrio campbellii strain DS40M4

40.802

100

0.424
  pilQ Neisseria gonorrhoeae strain FA1090

37.5

100

0.397
  pilQ Neisseria meningitidis MC58

37.587

100

0.397
  pilQ Neisseria meningitidis M1080

37.587

100

0.397

Multiple sequence alignment