Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   GKC13_RS00855 Genome accession   NZ_CP046134
Coordinates   152844..153785 (+) Length   313 a.a.
NCBI ID   WP_071417491.1    Uniprot ID   -
Organism   Streptococcus thermophilus strain MAG_rmk202_sterm     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 147844..158785
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKC13_RS00845 (GKC13_00840) rpoC 148604..152242 (+) 3639 WP_011226631.1 DNA-directed RNA polymerase subunit beta' -
  GKC13_RS00850 (GKC13_00845) - 152401..152763 (+) 363 WP_011226630.1 DUF1033 family protein -
  GKC13_RS00855 (GKC13_00850) comGA/cglA/cilD 152844..153785 (+) 942 WP_071417491.1 competence type IV pilus ATPase ComGA Machinery gene
  GKC13_RS00860 (GKC13_00855) comYB 153667..154767 (+) 1101 WP_207560111.1 competence type IV pilus assembly protein ComGB Machinery gene
  GKC13_RS00865 (GKC13_00860) comYC 154764..155090 (+) 327 WP_002946126.1 competence type IV pilus major pilin ComGC Machinery gene
  GKC13_RS00870 (GKC13_00865) comYD 155050..155478 (+) 429 WP_011226626.1 competence type IV pilus minor pilin ComGD Machinery gene
  GKC13_RS00875 (GKC13_00870) comGE 155450..155743 (+) 294 WP_011226625.1 competence type IV pilus minor pilin ComGE -
  GKC13_RS00880 (GKC13_00875) comYF 155727..156164 (+) 438 WP_011681686.1 competence type IV pilus minor pilin ComGF Machinery gene
  GKC13_RS00885 (GKC13_00880) comGG 156142..156459 (+) 318 WP_011681685.1 competence type IV pilus minor pilin ComGG -
  GKC13_RS00890 (GKC13_00885) comYH 156504..157469 (+) 966 WP_208301810.1 class I SAM-dependent methyltransferase Machinery gene
  GKC13_RS00895 (GKC13_00890) - 157515..158708 (+) 1194 WP_014608740.1 acetate kinase -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35411.58 Da        Isoelectric Point: 6.4142

>NTDB_id=401490 GKC13_RS00855 WP_071417491.1 152844..153785(+) (comGA/cglA/cilD) [Streptococcus thermophilus strain MAG_rmk202_sterm]
MVTEFAKEMIKNADSCGAQDIYVIPRQDNYELYMRVGQERRLIDVYRPDFMASLIGHFKFVAGMMVGEKRRSQLGSCDYD
CGDGHLVSLRLSTVGDYRGLESLVIRILHSERRELVYWNQGIHPIKDALDYRGLYLFAGPVGSGKTTLMHELVQEHFKGQ
QVISIEDPVEIKQDNVLQLQVNQAIDMTYDSLIKLSLRHRPDVLIIGEIRDKETARAVIRASLIGVTVLSTIHAKSIAGV
YERLLDLGVDKSELDNALQGIAYMRLIKGGGVIDFARENFQNHSSTNWNQQLEGLVKQGCLTERDIQGEKIKD

Nucleotide


Download         Length: 942 bp        

>NTDB_id=401490 GKC13_RS00855 WP_071417491.1 152844..153785(+) (comGA/cglA/cilD) [Streptococcus thermophilus strain MAG_rmk202_sterm]
ATGGTAACAGAATTTGCTAAGGAAATGATCAAAAATGCTGATAGTTGTGGGGCTCAAGACATCTATGTCATTCCACGTCA
GGATAATTATGAGCTCTATATGCGAGTCGGGCAAGAGAGGAGATTGATTGATGTATATCGGCCTGATTTCATGGCTAGTC
TTATTGGTCACTTTAAATTTGTGGCGGGAATGATGGTGGGTGAGAAGCGTAGGAGTCAACTGGGTTCATGTGACTATGAT
TGTGGTGACGGTCACTTGGTTTCTCTGCGTTTATCAACTGTTGGAGACTATCGAGGTTTGGAGAGCTTGGTTATCCGTAT
CCTACATTCCGAACGTCGAGAATTAGTGTATTGGAATCAAGGAATCCATCCTATTAAGGATGCCTTGGATTATAGAGGAC
TGTATCTCTTTGCGGGTCCCGTAGGTTCTGGGAAGACCACACTTATGCATGAGTTGGTTCAGGAGCATTTTAAGGGGCAG
CAGGTAATTTCGATTGAGGATCCTGTTGAAATCAAACAGGATAATGTCTTGCAACTCCAGGTTAATCAGGCGATTGATAT
GACCTATGATAGTTTGATTAAGCTATCTCTACGTCACCGTCCAGATGTTTTGATTATTGGGGAGATTCGGGATAAGGAGA
CTGCCCGGGCAGTTATTAGAGCTAGTCTAATAGGTGTAACTGTTCTTTCAACTATTCATGCTAAGAGTATTGCTGGCGTT
TACGAACGTCTCTTGGACCTTGGCGTAGATAAGTCTGAGTTGGATAATGCTCTACAAGGCATTGCCTACATGCGTTTGAT
CAAGGGAGGAGGTGTGATTGATTTTGCCAGAGAAAATTTCCAAAACCATTCGTCGACCAACTGGAATCAGCAGTTGGAAG
GTTTGGTTAAACAAGGATGTCTCACTGAGAGGGATATCCAAGGGGAAAAAATTAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

65.273

99.361

0.649
  comYA Streptococcus mutans UA159

64.217

100

0.642
  comYA Streptococcus mutans UA140

64.217

100

0.642
  comGA/cglA/cilD Streptococcus pneumoniae D39

63.987

99.361

0.636
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

63.987

99.361

0.636
  comGA/cglA/cilD Streptococcus pneumoniae R6

63.987

99.361

0.636
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

63.987

99.361

0.636
  comYA Streptococcus gordonii str. Challis substr. CH1

62.939

100

0.629
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

61.218

99.681

0.61
  comGA Lactococcus lactis subsp. cremoris KW2

51.125

99.361

0.508
  comGA Latilactobacillus sakei subsp. sakei 23K

38.667

95.847

0.371

Multiple sequence alignment