Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   GKC13_RS00860 Genome accession   NZ_CP046134
Coordinates   153667..154767 (+) Length   366 a.a.
NCBI ID   WP_207560111.1    Uniprot ID   -
Organism   Streptococcus thermophilus strain MAG_rmk202_sterm     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 148667..159767
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GKC13_RS00850 (GKC13_00845) - 152401..152763 (+) 363 WP_011226630.1 DUF1033 family protein -
  GKC13_RS00855 (GKC13_00850) comGA/cglA/cilD 152844..153785 (+) 942 WP_071417491.1 competence type IV pilus ATPase ComGA Machinery gene
  GKC13_RS00860 (GKC13_00855) comYB 153667..154767 (+) 1101 WP_207560111.1 competence type IV pilus assembly protein ComGB Machinery gene
  GKC13_RS00865 (GKC13_00860) comYC 154764..155090 (+) 327 WP_002946126.1 competence type IV pilus major pilin ComGC Machinery gene
  GKC13_RS00870 (GKC13_00865) comYD 155050..155478 (+) 429 WP_011226626.1 competence type IV pilus minor pilin ComGD Machinery gene
  GKC13_RS00875 (GKC13_00870) comGE 155450..155743 (+) 294 WP_011226625.1 competence type IV pilus minor pilin ComGE -
  GKC13_RS00880 (GKC13_00875) comYF 155727..156164 (+) 438 WP_011681686.1 competence type IV pilus minor pilin ComGF Machinery gene
  GKC13_RS00885 (GKC13_00880) comGG 156142..156459 (+) 318 WP_011681685.1 competence type IV pilus minor pilin ComGG -
  GKC13_RS00890 (GKC13_00885) comYH 156504..157469 (+) 966 WP_208301810.1 class I SAM-dependent methyltransferase Machinery gene
  GKC13_RS00895 (GKC13_00890) - 157515..158708 (+) 1194 WP_014608740.1 acetate kinase -
  GKC13_RS00900 (GKC13_00895) - 158957..159154 (+) 198 WP_014727723.1 helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 41789.94 Da        Isoelectric Point: 10.1952

>NTDB_id=401491 GKC13_RS00860 WP_207560111.1 153667..154767(+) (comYB) [Streptococcus thermophilus strain MAG_rmk202_sterm]
MPEKISKTIRRPTGISSWKVWLNKDVSLRGISKGKKLKISQQVKVIQLFKQLLKAGFTLTEIVAFLERSHLLKETSLSLM
KESLIRGDRLYQMLALVGFSDNIVTQISLADKHGNLLGSLTKIETYMLRMTKVRKKLMGVATYPILLLGFLILIMLGLKN
YLLPQLLEGDGKNNWAVQLVQIFPQLFFVSLCGLLVLGLILYLWVKRQSALVFYRRMAKIPFIGQTVRLYTTAYYAREWG
NLLGQGVDLLDLVALMQEQKSKLFRDLGADLEEALMLGQSFPERIASHPFFTKELSLIIAYGEANARLGYELEVYAEEVW
QNFFNRLNKATTFVQPLIFVIVAVVIVMIYVAMLLPMYQNMEGMMS

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=401491 GKC13_RS00860 WP_207560111.1 153667..154767(+) (comYB) [Streptococcus thermophilus strain MAG_rmk202_sterm]
TTGCCAGAGAAAATTTCCAAAACCATTCGTCGACCAACTGGAATCAGCAGTTGGAAGGTTTGGTTAAACAAGGATGTCTC
ACTGAGAGGGATATCCAAGGGGAAAAAATTAAAGATTAGTCAGCAAGTTAAGGTCATCCAGCTCTTCAAACAACTTTTAA
AGGCCGGTTTTACCTTAACTGAAATCGTAGCCTTTTTGGAGCGAAGTCACTTGCTGAAAGAAACATCCTTGTCTCTTATG
AAAGAGAGTTTAATACGCGGTGATAGGTTGTATCAGATGTTAGCGTTAGTGGGGTTTTCGGACAATATTGTTACTCAGAT
TTCTCTTGCTGACAAGCACGGTAATCTTCTAGGGAGTCTAACAAAGATTGAAACCTATATGCTTCGTATGACAAAGGTTC
GCAAGAAACTCATGGGGGTGGCGACTTATCCCATACTACTTCTGGGTTTTCTGATTCTGATTATGCTAGGACTTAAAAAT
TATCTTCTACCCCAACTCTTAGAGGGTGATGGTAAGAATAATTGGGCTGTACAGTTGGTTCAAATTTTTCCCCAGCTTTT
TTTTGTGAGTTTGTGTGGACTCCTTGTGTTGGGTTTAATTCTCTATCTATGGGTGAAACGACAGTCAGCCCTTGTTTTTT
ATAGGCGAATGGCCAAAATCCCTTTTATTGGTCAGACAGTAAGGCTTTACACGACCGCCTATTATGCTAGGGAATGGGGA
AATTTATTAGGTCAAGGCGTTGATTTGCTAGATTTGGTGGCTCTTATGCAAGAGCAGAAGTCTAAACTCTTCCGTGATCT
GGGAGCCGATTTGGAAGAAGCCCTGATGCTGGGACAGAGTTTTCCAGAACGTATTGCCAGTCATCCGTTTTTTACTAAGG
AGCTCTCACTAATTATTGCTTATGGGGAGGCCAATGCGAGGTTGGGCTATGAGTTGGAAGTTTATGCCGAGGAGGTTTGG
CAAAACTTCTTTAACCGTCTTAATAAGGCAACAACCTTTGTGCAACCCCTCATTTTTGTTATTGTTGCAGTTGTGATTGT
AATGATCTATGTAGCCATGCTATTACCAATGTATCAAAATATGGAAGGAATGATGTCATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(236-356)

Type II secretion system (T2SS), protein F (T2SSF)

(51-164)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

54.094

93.443

0.505
  comYB Streptococcus mutans UA159

54.094

93.443

0.505
  comYB Streptococcus gordonii str. Challis substr. CH1

50.585

93.443

0.473
  comGB/cglB Streptococcus mitis NCTC 12261

49.849

90.437

0.451
  comGB/cglB Streptococcus mitis SK321

49.091

90.164

0.443
  comGB/cglB Streptococcus pneumoniae Rx1

48.788

90.164

0.44
  comGB/cglB Streptococcus pneumoniae D39

48.788

90.164

0.44
  comGB/cglB Streptococcus pneumoniae R6

48.788

90.164

0.44
  comGB/cglB Streptococcus pneumoniae TIGR4

48.788

90.164

0.44
  comGB Lactococcus lactis subsp. cremoris KW2

45.152

90.164

0.407

Multiple sequence alignment