Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   VARPA_RS27330 Genome accession   NC_014931
Coordinates   5956561..5957697 (+) Length   378 a.a.
NCBI ID   WP_013543831.1    Uniprot ID   E6V9E6
Organism   Variovorax paradoxus EPS     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5951561..5962697
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VARPA_RS27305 (Varpa_5470) - 5952149..5953420 (-) 1272 WP_013543826.1 Glu/Leu/Phe/Val family dehydrogenase -
  VARPA_RS27310 (Varpa_5471) - 5953658..5954113 (+) 456 WP_013543827.1 hypothetical protein -
  VARPA_RS27315 (Varpa_5472) - 5954256..5954723 (+) 468 WP_013543828.1 Lrp/AsnC ligand binding domain-containing protein -
  VARPA_RS27320 (Varpa_5473) - 5954734..5955435 (-) 702 WP_013543829.1 YggS family pyridoxal phosphate-dependent enzyme -
  VARPA_RS27325 (Varpa_5474) pilT 5955487..5956530 (+) 1044 WP_013543830.1 type IV pilus twitching motility protein PilT Machinery gene
  VARPA_RS27330 (Varpa_5475) pilU 5956561..5957697 (+) 1137 WP_013543831.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VARPA_RS27335 (Varpa_5476) - 5957764..5958672 (+) 909 WP_013543832.1 NAD(P)-dependent oxidoreductase -
  VARPA_RS27340 (Varpa_5477) - 5958703..5959515 (-) 813 WP_013543833.1 BON domain-containing protein -
  VARPA_RS27345 (Varpa_5478) - 5959512..5960156 (-) 645 WP_013543834.1 SIS domain-containing protein -
  VARPA_RS27350 (Varpa_5479) - 5960243..5960617 (-) 375 WP_013543835.1 YraN family protein -
  VARPA_RS27355 (Varpa_5480) rsmI 5960669..5961625 (+) 957 WP_013543836.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  VARPA_RS27360 (Varpa_5481) - 5961622..5962350 (-) 729 WP_013543837.1 glutathione S-transferase -
  VARPA_RS27365 (Varpa_5482) - 5962428..5962649 (-) 222 WP_041944048.1 hypothetical protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41866.93 Da        Isoelectric Point: 6.4186

>NTDB_id=39553 VARPA_RS27330 WP_013543831.1 5956561..5957697(+) (pilU) [Variovorax paradoxus EPS]
MERDQASQFINDLLKLMVSRNGSDLFITADFPPAIKVDGKVTKVSQQALGAQHTLALTRSVMNDRQVADFERTKECNFAI
SPTGIGRFRVNAFVQQGKVGMVLRTIPAKLPTIDGLGMPQILKDVSMTKRGLTILVGATGSGKSTTLAAMIDWRNENSYG
HIVTVEDPVEFVHPHKNCVITQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSLVSQRLVPTEDGQGRVAAVEVLLNTPLISDLIFKGEVGEIKEIMRKSRNLG
MQTFDQALFDLFESHSITFEDAIRNADSANDLRLQIKLNSQRARSTDLAAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=39553 VARPA_RS27330 WP_013543831.1 5956561..5957697(+) (pilU) [Variovorax paradoxus EPS]
ATGGAACGCGATCAAGCCAGCCAGTTCATCAACGACCTGCTCAAGCTCATGGTGAGCCGCAACGGCAGCGACCTGTTCAT
CACCGCCGACTTTCCGCCCGCGATCAAGGTCGACGGCAAGGTCACCAAGGTGTCGCAGCAAGCGCTGGGCGCGCAGCACA
CGCTGGCGCTCACGCGCTCGGTCATGAACGACCGGCAGGTGGCCGATTTCGAGCGCACCAAGGAGTGCAACTTCGCGATC
TCGCCGACCGGCATCGGGCGCTTTCGCGTCAACGCCTTCGTGCAGCAGGGCAAGGTCGGCATGGTGCTGCGGACCATTCC
GGCCAAGCTGCCGACCATCGACGGCCTGGGCATGCCGCAGATCCTGAAGGACGTGTCGATGACCAAGCGCGGCCTCACCA
TCCTGGTGGGCGCGACGGGCTCGGGCAAGTCGACCACGCTGGCCGCGATGATCGATTGGCGCAACGAAAACTCCTACGGC
CACATCGTCACGGTGGAAGACCCGGTCGAGTTCGTGCACCCGCACAAGAACTGCGTGATCACGCAGCGCGAAGTGGGCAT
CGACACCGACAGCTGGGAAGCCGCGCTCAAGAACACGCTGCGCCAAGCGCCCGACGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAAACCATGGAGCACGCGGTGGCCTTCGCCGAAACGGGCCACCTCTGCATGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGGATCATCAACTTCTTCCCCGAAGAGCGCCGCGCTCAACTGCTGATGGACCTCTCGCTGAACCT
GCGCTCGCTGGTCTCGCAGCGGCTGGTGCCGACCGAAGACGGCCAGGGCCGCGTGGCCGCCGTCGAGGTGCTGCTGAACA
CGCCGCTGATTTCCGACCTGATCTTCAAGGGCGAGGTCGGCGAGATCAAGGAGATCATGCGCAAGAGCCGCAATCTGGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTGTTCGAGAGCCACTCGATCACCTTCGAAGACGCGATCCGCAACGCCGA
CTCGGCCAACGACCTGCGGCTGCAGATCAAGCTCAACAGCCAGCGTGCGCGCAGCACCGACCTGGCGGCCGGCACGGAGC
ATTTCGCGATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(50-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E6V9E6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.324

92.328

0.585
  pilU Acinetobacter baylyi ADP1

57.821

94.709

0.548
  pilU Vibrio cholerae strain A1552

52.066

96.032

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.291

89.153

0.413
  pilT Pseudomonas aeruginosa PAK

44.51

89.153

0.397
  pilT Acinetobacter nosocomialis M2

44.444

88.095

0.392
  pilT Acinetobacter baumannii strain A118

45.455

84.392

0.384
  pilT Acinetobacter baumannii D1279779

45.455

84.392

0.384
  pilT Pseudomonas stutzeri DSM 10701

42.433

89.153

0.378
  pilT Acinetobacter baylyi ADP1

43.963

85.45

0.376
  pilT Legionella pneumophila strain Lp02

43.161

87.037

0.376
  pilT Legionella pneumophila strain ERS1305867

43.161

87.037

0.376

Multiple sequence alignment