Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilE   Type   Machinery gene
Locus tag   NLA_RS00960 Genome accession   NC_014752
Coordinates   182171..182617 (-) Length   148 a.a.
NCBI ID   WP_013448269.1    Uniprot ID   E4ZAF9
Organism   Neisseria lactamica 020-06     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 177171..187617
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NLA_RS13060 - 177408..177635 (-) 228 WP_042508111.1 hypothetical protein -
  NLA_RS00945 (NLA_1750) - 177675..178547 (+) 873 WP_013448266.1 mechanosensitive ion channel family protein -
  NLA_RS00950 (NLA_1760) - 178635..180671 (+) 2037 WP_013448267.1 M3 family metallopeptidase -
  NLA_RS00955 (NLA_1770) - 181096..181662 (-) 567 WP_013448268.1 manganese efflux pump MntP family protein -
  NLA_RS00960 (NLA_1780) pilE 182171..182617 (-) 447 WP_013448269.1 pilin Machinery gene
  NLA_RS13065 - 183467..183631 (+) 165 WP_155270588.1 hypothetical protein -
  NLA_RS13250 - 183701..183889 (-) 189 WP_162467539.1 hypothetical protein -
  NLA_RS00970 (NLA_1790) katA 183966..185480 (-) 1515 WP_013448270.1 catalase KatA -
  NLA_RS00975 (NLA_1800) rpoN 185829..187172 (+) 1344 WP_042508291.1 RNA polymerase factor sigma-54 -

Sequence


Protein


Download         Length: 148 a.a.        Molecular weight: 15448.74 Da        Isoelectric Point: 8.9718

>NTDB_id=39050 NLA_RS00960 WP_013448269.1 182171..182617(-) (pilE) [Neisseria lactamica 020-06]
MKAIQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQMSEALTLAEGQKAAVVEYYSDNGTFPAQNASAGIATASAIT
GKYVAKVEVTGDATSATITSTMKSSGVNNDIKGKTLVLVGKQNSGSFSWECKKGTVDDKFLPSSCRAK

Nucleotide


Download         Length: 447 bp        

>NTDB_id=39050 NLA_RS00960 WP_013448269.1 182171..182617(-) (pilE) [Neisseria lactamica 020-06]
ATGAAAGCAATCCAAAAAGGTTTCACCCTGATCGAGCTGATGATCGTCATCGCCATCGTCGGTATCTTGGCAGCCGTCGC
CCTGCCCGCATACCAAGACTACACCGCGCGCGCCCAAATGTCCGAAGCCCTGACTTTGGCTGAAGGTCAAAAAGCCGCAG
TGGTCGAGTATTATTCCGACAACGGTACATTCCCTGCCCAAAATGCCTCTGCAGGTATTGCTACTGCTTCTGCAATTACA
GGTAAATATGTGGCTAAAGTAGAAGTTACAGGTGATGCTACCTCTGCCACCATTACCTCTACTATGAAATCTTCCGGTGT
AAATAACGACATCAAAGGTAAAACTTTGGTACTCGTCGGCAAACAAAACTCCGGCTCGTTCTCTTGGGAATGTAAAAAAG
GTACTGTGGACGACAAATTCCTGCCGTCTTCCTGCCGCGCCAAATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB E4ZAF9

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

57.576

100

0.642

  pilE Neisseria gonorrhoeae strain FA1090

56.79

100

0.622

  pilA/pilA1 Eikenella corrodens VA1

43.125

100

0.466

  pilE Neisseria elongata subsp. glycolytica ATCC 29315

35.979

100

0.459

  pilA Ralstonia pseudosolanacearum GMI1000

38.69

100

0.439

  pilA2 Legionella pneumophila str. Paris

44.366

95.946

0.426

  pilA2 Legionella pneumophila strain ERS1305867

43.662

95.946

0.419

  comP Acinetobacter baylyi ADP1

41.216

100

0.412

  pilA Pseudomonas aeruginosa PAK

37.662

100

0.392

  pilA Acinetobacter baumannii strain A118

37.838

100

0.378

  pilA/pilAI Pseudomonas stutzeri DSM 10701

36.986

98.649

0.365

  pilA Acinetobacter nosocomialis M2

47.368

77.027

0.365


Multiple sequence alignment