Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   NWAT_RS12380 Genome accession   NC_014315
Coordinates   2724741..2725169 (-) Length   142 a.a.
NCBI ID   WP_013221397.1    Uniprot ID   D8K9X0
Organism   Nitrosococcus watsonii C-113     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2719741..2730169
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NWAT_RS12360 (Nwat_2535) - 2720086..2720811 (-) 726 WP_013221393.1 TylF/MycF/NovP-related O-methyltransferase -
  NWAT_RS15740 (Nwat_2536) - 2720901..2721668 (-) 768 WP_013221394.1 class I SAM-dependent methyltransferase -
  NWAT_RS12370 (Nwat_2537) - 2721728..2722597 (-) 870 WP_013221395.1 glycosyltransferase family 2 protein -
  NWAT_RS12375 (Nwat_2538) - 2722685..2724634 (-) 1950 WP_013221396.1 M48 family metallopeptidase -
  NWAT_RS12380 (Nwat_2539) pilA2 2724741..2725169 (-) 429 WP_013221397.1 pilin Machinery gene
  NWAT_RS12385 (Nwat_2540) - 2725616..2725864 (+) 249 WP_013221398.1 type II toxin-antitoxin system ParD family antitoxin -
  NWAT_RS12390 (Nwat_2541) - 2725864..2726160 (+) 297 WP_013221399.1 type II toxin-antitoxin system RelE/ParE family toxin -
  NWAT_RS17590 (Nwat_2542) - 2726584..2726961 (+) 378 WP_232420127.1 hypothetical protein -
  NWAT_RS12400 (Nwat_2543) - 2726910..2727407 (+) 498 WP_041350739.1 helicase-related protein -
  NWAT_RS12405 (Nwat_2544) - 2727451..2728026 (+) 576 WP_013221400.1 transposase -
  NWAT_RS12410 (Nwat_2545) - 2727990..2729144 (+) 1155 WP_013221401.1 C4-dicarboxylate transporter/malic acid transport protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 14734.95 Da        Isoelectric Point: 8.4502

>NTDB_id=37777 NWAT_RS12380 WP_013221397.1 2724741..2725169(-) (pilA2) [Nitrosococcus watsonii C-113]
MRTVQQGFTLIELMIVVAIIGILAAVAIPAYQDYTIRAKVSEPLSFGDSAKVSVSEYFQAVGAMPTTMSAAGISSVSSPY
ISSIAYTNTGSVGATITVATKNLGNSVTGNIVLDGTGKTDGTVTWICTNSTLAKKYLPANCR

Nucleotide


Download         Length: 429 bp        

>NTDB_id=37777 NWAT_RS12380 WP_013221397.1 2724741..2725169(-) (pilA2) [Nitrosococcus watsonii C-113]
ATGAGAACAGTACAACAGGGTTTTACCTTGATCGAACTCATGATCGTGGTGGCGATCATCGGTATTTTAGCCGCTGTGGC
GATTCCGGCCTATCAAGACTACACAATACGGGCTAAGGTCTCTGAGCCCTTGAGTTTTGGCGATTCAGCCAAGGTATCTG
TAAGTGAATACTTTCAAGCTGTGGGTGCGATGCCAACAACTATGAGCGCTGCCGGCATTTCTAGTGTGAGTTCACCATAC
ATTAGTAGTATAGCTTACACTAACACCGGTTCTGTGGGAGCAACGATTACTGTGGCCACGAAGAATTTAGGAAATAGTGT
TACGGGTAACATTGTACTGGATGGTACAGGCAAGACAGATGGCACGGTTACTTGGATTTGTACGAATAGCACTCTTGCCA
AAAAATACCTACCCGCGAATTGTCGCTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-142)

Prokaryotic N-terminal methylation motif (N_methyl)

(4-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D8K9X0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

53.237

97.887

0.521
  pilA2 Legionella pneumophila strain ERS1305867

52.518

97.887

0.514
  comP Acinetobacter baylyi ADP1

47.368

100

0.507
  pilA Ralstonia pseudosolanacearum GMI1000

40.606

100

0.472
  pilA Acinetobacter baumannii strain A118

44.056

100

0.444
  pilE Neisseria gonorrhoeae MS11

38.272

100

0.437
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

32.461

100

0.437
  pilE Neisseria gonorrhoeae strain FA1090

36.076

100

0.401
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.732

100

0.387
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.194

100

0.387
  pilA Glaesserella parasuis strain SC1401

36.242

100

0.38
  pilA Haemophilus influenzae 86-028NP

38.028

100

0.38

Multiple sequence alignment