Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   DZ927_RS26715 Genome accession   NZ_CP039293
Coordinates   5560933..5561385 (-) Length   150 a.a.
NCBI ID   WP_019486070.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PABL048     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5555933..5566385
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DZ927_RS26695 (DZ927_026695) ampD 5556628..5557194 (-) 567 WP_003112844.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  DZ927_RS26700 (DZ927_026700) - 5557345..5559600 (-) 2256 WP_016264271.1 DUF1631 domain-containing protein -
  DZ927_RS26705 (DZ927_026705) nadC 5559886..5560734 (+) 849 WP_003161760.1 carboxylating nicotinate-nucleotide diphosphorylase -
  DZ927_RS26715 (DZ927_026715) pilA 5560933..5561385 (-) 453 WP_019486070.1 pilin Machinery gene
  DZ927_RS26720 (DZ927_026720) pilB 5561617..5563317 (+) 1701 WP_023094327.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DZ927_RS26725 (DZ927_026725) pilC 5563321..5564541 (+) 1221 WP_003112840.1 type 4a pilus biogenesis protein PilC Machinery gene
  DZ927_RS26730 (DZ927_026730) pilD 5564545..5565417 (+) 873 WP_019486071.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  DZ927_RS26735 (DZ927_026735) coaE 5565414..5566025 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  DZ927_RS26740 (DZ927_026740) yacG 5566022..5566222 (+) 201 WP_019486072.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15757.14 Da        Isoelectric Point: 8.4742

>NTDB_id=357925 DZ927_RS26715 WP_019486070.1 5560933..5561385(-) (pilA) [Pseudomonas aeruginosa strain PABL048]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKILIGTTASTADTTYV
GIDEKANKLGTVAVTIKDTGDGTIKFTFATGQSSPKNAGKEITLNRTAEGVWTCTSTQEEMFIPKGCNKP

Nucleotide


Download         Length: 453 bp        

>NTDB_id=357925 DZ927_RS26715 WP_019486070.1 5560933..5561385(-) (pilA) [Pseudomonas aeruginosa strain PABL048]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAGCTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCGATTGCCAT
TCCCCAGTACCAGAACTATGTGGCGCGTTCGGAAGGCGCTTCGGCGCTGGCGACGATCAACCCGTTGAAGACCACTGTTG
AAGAGTCGCTGTCGCGTGGAATTGCTGGTAGCAAAATTCTGATCGGTACTACAGCTTCTACTGCAGATACCACCTATGTA
GGTATTGATGAGAAGGCAAATAAACTTGGTACCGTAGCTGTAACTATTAAAGACACAGGCGATGGTACTATAAAATTTAC
TTTTGCAACTGGTCAGTCCAGTCCGAAGAATGCGGGCAAGGAAATTACTTTGAATCGTACTGCTGAAGGTGTATGGACTT
GCACCTCTACTCAGGAAGAGATGTTTATTCCTAAGGGTTGTAATAAGCCTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

67.105

100

0.68
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

45.625

100

0.487
  pilA Vibrio cholerae strain A1552

45.625

100

0.487
  pilA Vibrio cholerae C6706

45.625

100

0.487
  pilA/pilAI Pseudomonas stutzeri DSM 10701

46.667

100

0.467
  pilA Acinetobacter baumannii strain A118

45.27

98.667

0.447
  comP Acinetobacter baylyi ADP1

42.484

100

0.433
  pilA Vibrio parahaemolyticus RIMD 2210633

42.568

98.667

0.42
  pilA/pilAII Pseudomonas stutzeri DSM 10701

44.056

95.333

0.42
  pilA/pilA1 Eikenella corrodens VA1

40.541

98.667

0.4
  pilA2 Legionella pneumophila strain ERS1305867

38.667

100

0.387
  pilA2 Legionella pneumophila str. Paris

38.926

99.333

0.387

Multiple sequence alignment