Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   E4K03_RS17460 Genome accession   NZ_CP038262
Coordinates   3567817..3568617 (-) Length   266 a.a.
NCBI ID   WP_005135836.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain LEV1449/17Ec     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3562817..3573617
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4K03_RS17440 (E4K03_17440) - 3563882..3564580 (-) 699 WP_000058788.1 tetratricopeptide repeat protein -
  E4K03_RS17445 (E4K03_17445) hisS 3564609..3565901 (-) 1293 WP_000095251.1 histidine--tRNA ligase -
  E4K03_RS17450 (E4K03_17450) ispG 3565898..3567013 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  E4K03_RS17455 (E4K03_17455) - 3567035..3567826 (-) 792 WP_000405163.1 RodZ domain-containing protein -
  E4K03_RS17460 (E4K03_17460) pilF 3567817..3568617 (-) 801 WP_005135836.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  E4K03_RS17465 (E4K03_17465) rlmN 3568633..3569865 (-) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  E4K03_RS17470 (E4K03_17470) ndk 3569988..3570419 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  E4K03_RS17475 (E4K03_17475) iscX 3570533..3570733 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  E4K03_RS17480 (E4K03_17480) - 3570833..3571384 (-) 552 WP_000669563.1 phosphatase PAP2 family protein -
  E4K03_RS17485 (E4K03_17485) - 3571409..3572707 (-) 1299 WP_086238243.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30120.52 Da        Isoelectric Point: 9.2307

>NTDB_id=352885 E4K03_RS17460 WP_005135836.1 3567817..3568617(-) (pilF) [Acinetobacter baumannii strain LEV1449/17Ec]
MSTPKLKIMLCMGVAVALLASGCQTLQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=352885 E4K03_RS17460 WP_005135836.1 3567817..3568617(-) (pilF) [Acinetobacter baumannii strain LEV1449/17Ec]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGTCAGACTTTGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCCCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCCTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.624

100

0.996
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726

Multiple sequence alignment