Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   EWA64_RS07210 Genome accession   NZ_CP035921
Coordinates   1360460..1362235 (-) Length   591 a.a.
NCBI ID   WP_061895832.1    Uniprot ID   A0A151KYU1
Organism   Vibrio cidicii strain 2423-01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1355460..1367235
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EWA64_RS07190 - 1356144..1356971 (-) 828 WP_061895835.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  EWA64_RS07195 - 1357051..1358556 (-) 1506 WP_061895834.1 AAA family ATPase -
  EWA64_RS07200 aroB 1358608..1359699 (-) 1092 WP_061895833.1 3-dehydroquinate synthase -
  EWA64_RS07205 aroK 1359732..1360250 (-) 519 WP_039423021.1 shikimate kinase AroK -
  EWA64_RS07210 pilQ 1360460..1362235 (-) 1776 WP_061895832.1 type IV pilus secretin PilQ Machinery gene
  EWA64_RS07215 pilP 1362266..1362778 (-) 513 WP_039438951.1 pilus assembly protein PilP Machinery gene
  EWA64_RS07220 pilO 1362768..1363358 (-) 591 WP_061895831.1 type 4a pilus biogenesis protein PilO Machinery gene
  EWA64_RS07225 pilN 1363351..1363938 (-) 588 WP_061895830.1 PilN domain-containing protein Machinery gene
  EWA64_RS07230 pilM 1363922..1364941 (-) 1020 WP_061895829.1 type IV pilus biogenesis protein PilM Machinery gene

Sequence

Protein


Download         Length: 591 a.a.        Molecular weight: 64102.17 Da        Isoelectric Point: 4.7445

>NTDB_id=344990 EWA64_RS07210 WP_061895832.1 1360460..1362235(-) (pilQ) [Vibrio cidicii strain 2423-01]
MTMKKGMIRLQAPLRQLMCLFALCLFAAFANAQPENNSQSASENALKSIDFRTNQNKAAVIIVELVSPAAVVDLKPVQEG
LSIELLNTRVSDEQLYLLDVKDFSTPVESVEVFRDVSTTRLLASVSGEFRHEYRLTGRYLEVTVSRIETPQQVEEKSILE
KEGKLISINFQDIPVRNVLQLIADYNGFNLVVSDSVTGNLTLRLDGVPWQQVLDIILQVKGLDKRVEGNVVLIAPKEELD
LREKQHLEKQRLAEELGSLKSEIIKVNFAKASDIAEMISGTESVSMLSERGSIAIDERTNSLLLRELPENIAVIREIVNS
LDIPVKQVQIEARIVTVNEGNLDELGVRWGFTSVNGSHTVGGSIENNLASVGLYNAAGGGSGSGGDSGGGSSSIDDFLNV
NLGATSGNASSIAFQVAKLGSDLLLDLELSALQRESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVSFKKA
VLSLKVTPQITPDNRLVLDLSVTQDRPGEVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSVTSTVDKVPLLGD
LPLLGALFRRSYENIGKRELLIFVTPKVVIQ

Nucleotide


Download         Length: 1776 bp        

>NTDB_id=344990 EWA64_RS07210 WP_061895832.1 1360460..1362235(-) (pilQ) [Vibrio cidicii strain 2423-01]
ATGACCATGAAGAAAGGAATGATTCGATTACAGGCTCCGCTACGCCAATTAATGTGCCTGTTTGCTCTCTGCCTCTTTGC
CGCGTTTGCAAACGCGCAGCCAGAGAATAACAGTCAGTCAGCGAGTGAGAATGCGCTGAAAAGCATTGATTTTAGAACCA
ACCAGAATAAAGCCGCGGTGATCATTGTCGAGTTGGTTTCGCCTGCCGCGGTGGTGGACCTTAAACCTGTGCAGGAGGGG
CTTAGCATTGAGCTGCTCAACACCCGCGTCAGCGACGAACAGCTCTATCTATTAGACGTTAAAGACTTCTCAACGCCAGT
TGAAAGTGTTGAAGTGTTCCGTGATGTGTCAACGACGCGTTTGCTGGCGTCTGTCTCGGGGGAGTTTCGTCATGAATATC
GTTTGACAGGGCGCTATTTGGAAGTGACGGTCAGTCGTATAGAAACCCCGCAGCAAGTCGAGGAGAAAAGCATCCTAGAA
AAAGAGGGCAAGCTTATCTCGATCAACTTCCAAGACATTCCGGTGAGAAACGTGTTGCAACTGATAGCGGACTACAACGG
TTTTAACCTAGTGGTCTCCGATTCCGTCACGGGCAATCTGACCTTGCGCTTGGATGGCGTGCCGTGGCAGCAAGTGCTCG
ACATCATTTTGCAAGTCAAAGGGCTAGACAAGCGGGTGGAAGGCAATGTGGTGCTGATCGCGCCAAAAGAAGAGTTGGAC
TTGCGTGAGAAGCAGCATCTGGAAAAGCAGCGTTTGGCGGAAGAGTTAGGCTCACTCAAATCGGAAATTATCAAGGTGAA
CTTTGCCAAAGCGTCCGACATTGCCGAGATGATCAGCGGCACCGAGTCGGTCAGCATGCTGTCTGAGCGCGGTAGCATCG
CGATTGATGAACGTACGAACTCCTTACTGTTACGAGAGCTTCCGGAAAACATTGCGGTGATTCGTGAGATTGTCAATTCG
CTCGATATTCCGGTCAAACAGGTGCAAATTGAAGCACGCATTGTCACCGTCAATGAAGGCAATTTGGATGAGTTGGGCGT
GCGTTGGGGTTTTACCTCGGTCAACGGCAGCCACACGGTGGGCGGTTCGATCGAGAACAACTTGGCGAGCGTCGGTTTGT
ACAACGCGGCTGGTGGCGGTTCTGGCTCAGGAGGCGATAGCGGTGGCGGCTCCAGCAGCATCGACGATTTTCTCAACGTC
AATCTGGGGGCGACCTCCGGCAACGCATCAAGCATCGCTTTCCAAGTCGCCAAGCTGGGCTCGGATCTACTGCTTGATCT
AGAGCTGTCGGCGCTGCAGCGTGAGTCAAAAGCGGAGATCATTTCCAGCCCGCGTCTGATTACCACCAATAAAAAGCCAG
CCTACATCGAACAAGGGACCGAAATTCCCTATCTGGAATCGTCCTCCAGTGGCGCGACCACCGTTTCGTTCAAAAAAGCG
GTACTGAGTTTGAAAGTCACGCCGCAGATCACCCCGGATAACCGCTTGGTGCTTGATCTTAGCGTTACACAAGACAGGCC
GGGTGAAGTGGTGAAAACCGGTACGGGTGAAGCGGTGGCGATTGACACCCAACGTATCGGCACTCAGGTGTTGGTTAACA
ATGGTGAAACCGTGGTTCTCGGCGGCATTTTCCAACATAGCGTCACCAGTACGGTCGACAAAGTGCCTTTGCTGGGCGAT
CTGCCACTGCTTGGCGCGCTGTTTCGCCGCAGCTATGAAAATATCGGTAAACGGGAACTGTTGATCTTTGTGACGCCAAA
GGTGGTTATTCAATAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(262-327)

Bacterial type II and III secretion system protein (Secretin)

(431-589)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A151KYU1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

78.511

100

0.785
  pilQ Vibrio cholerae strain A1552

76.522

97.293

0.745
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

76.522

97.293

0.745
  comQ Acinetobacter baylyi ADP1

37.667

100

0.382
  pilQ Pseudomonas aeruginosa PAK

40.288

94.078

0.379
  pilQ Legionella pneumophila strain ERS1305867

39.964

93.57

0.374
  pilQ Legionella pneumophila strain Lp02

39.964

93.57

0.374
  pilQ Acinetobacter baumannii D1279779

36.486

100

0.365
  pilQ Acinetobacter baumannii strain A118

36.486

100

0.365

Multiple sequence alignment