Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   EWA64_RS07225 Genome accession   NZ_CP035921
Coordinates   1363351..1363938 (-) Length   195 a.a.
NCBI ID   WP_061895830.1    Uniprot ID   A0A151KYY3
Organism   Vibrio cidicii strain 2423-01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1358351..1368938
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EWA64_RS07200 aroB 1358608..1359699 (-) 1092 WP_061895833.1 3-dehydroquinate synthase -
  EWA64_RS07205 aroK 1359732..1360250 (-) 519 WP_039423021.1 shikimate kinase AroK -
  EWA64_RS07210 pilQ 1360460..1362235 (-) 1776 WP_061895832.1 type IV pilus secretin PilQ Machinery gene
  EWA64_RS07215 pilP 1362266..1362778 (-) 513 WP_039438951.1 pilus assembly protein PilP Machinery gene
  EWA64_RS07220 pilO 1362768..1363358 (-) 591 WP_061895831.1 type 4a pilus biogenesis protein PilO Machinery gene
  EWA64_RS07225 pilN 1363351..1363938 (-) 588 WP_061895830.1 PilN domain-containing protein Machinery gene
  EWA64_RS07230 pilM 1363922..1364941 (-) 1020 WP_061895829.1 type IV pilus biogenesis protein PilM Machinery gene
  EWA64_RS07235 - 1365080..1367596 (+) 2517 WP_061895828.1 penicillin-binding protein 1A -
  EWA64_RS07240 oxyR 1367670..1368563 (-) 894 WP_039436568.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 195 a.a.        Molecular weight: 22454.82 Da        Isoelectric Point: 7.5642

>NTDB_id=344993 EWA64_RS07225 WP_061895830.1 1363351..1363938(-) (pilN) [Vibrio cidicii strain 2423-01]
MKHTINLLPWREYQREEHRRRFVGVLILGVLIAVAIQWGIGQYVSYQQRLQQDRLNYLNHYIAQLDQRIQAMKIAKEEHG
QILARLKVVESLQAQRNNTTELMNIMPNVIPEGVYVDKIKMSDQEVEMAGISDSTARLATMLDNLERSEAIYDVEMHSIV
HGKERFGKAFQTFTVSFMFGVKPQSVQAVAEANHG

Nucleotide


Download         Length: 588 bp        

>NTDB_id=344993 EWA64_RS07225 WP_061895830.1 1363351..1363938(-) (pilN) [Vibrio cidicii strain 2423-01]
ATGAAGCACACCATTAATCTTCTGCCGTGGCGCGAATATCAGCGAGAGGAGCATCGACGCCGTTTTGTTGGGGTACTGAT
TTTAGGCGTGCTGATCGCTGTGGCGATTCAGTGGGGGATTGGCCAATACGTTAGTTATCAGCAGCGCTTACAGCAAGATC
GCCTCAACTACCTAAACCACTACATCGCCCAGTTGGATCAGCGCATTCAAGCGATGAAGATCGCTAAAGAAGAACACGGA
CAGATCTTAGCGCGTCTGAAGGTGGTGGAGTCACTGCAAGCGCAGCGTAATAACACCACGGAACTGATGAATATTATGCC
CAATGTCATTCCTGAAGGGGTTTACGTCGACAAGATCAAAATGAGCGACCAGGAAGTGGAAATGGCGGGTATCAGTGACA
GCACGGCGCGCCTTGCCACCATGTTGGACAATCTTGAGCGCTCGGAGGCGATTTACGACGTTGAGATGCACTCCATCGTG
CATGGCAAAGAGCGATTTGGTAAAGCGTTTCAGACCTTCACTGTCTCTTTTATGTTTGGCGTGAAGCCACAATCCGTGCA
GGCAGTGGCGGAGGCAAATCATGGTTGA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(102-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A151KYY3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

69.832

91.795

0.641
  pilN Vibrio cholerae strain A1552

57.949

100

0.579

Multiple sequence alignment