Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   EWA64_RS07230 Genome accession   NZ_CP035921
Coordinates   1363922..1364941 (-) Length   339 a.a.
NCBI ID   WP_061895829.1    Uniprot ID   -
Organism   Vibrio cidicii strain 2423-01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1358922..1369941
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EWA64_RS07205 aroK 1359732..1360250 (-) 519 WP_039423021.1 shikimate kinase AroK -
  EWA64_RS07210 pilQ 1360460..1362235 (-) 1776 WP_061895832.1 type IV pilus secretin PilQ Machinery gene
  EWA64_RS07215 pilP 1362266..1362778 (-) 513 WP_039438951.1 pilus assembly protein PilP Machinery gene
  EWA64_RS07220 pilO 1362768..1363358 (-) 591 WP_061895831.1 type 4a pilus biogenesis protein PilO Machinery gene
  EWA64_RS07225 pilN 1363351..1363938 (-) 588 WP_061895830.1 PilN domain-containing protein Machinery gene
  EWA64_RS07230 pilM 1363922..1364941 (-) 1020 WP_061895829.1 type IV pilus biogenesis protein PilM Machinery gene
  EWA64_RS07235 - 1365080..1367596 (+) 2517 WP_061895828.1 penicillin-binding protein 1A -
  EWA64_RS07240 oxyR 1367670..1368563 (-) 894 WP_039436568.1 DNA-binding transcriptional regulator OxyR -
  EWA64_RS07245 - 1368734..1368991 (+) 258 WP_061895827.1 DUF3624 domain-containing protein -

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 38204.00 Da        Isoelectric Point: 7.3958

>NTDB_id=344994 EWA64_RS07230 WP_061895829.1 1363922..1364941(-) (pilM) [Vibrio cidicii strain 2423-01]
MGKPLVTGIDIGHRSLKAVILKPAGNSYALLGYKEIVFERAIVAENHTLNHQEIVNTLKVLNKELPRFRRQVALAVPDSA
VISKQLQIEPIEDEKECEIAILQAFSHQSPFPVDELNMDYVRLHEPAQSKSASQSVQIFATKKEVVESRVRAAQAVRLKP
VLVDMHAHCLFNLRELAAQRYPDKSAFCLLDVGAQTTSLIIHQREKTHFIKEFAYGVEHLNAAIGDEFSAHDAQEKTERF
IVEMVDRVARQLQLFQSINSQDKPQGLWLCGEGAATPLLLEALSRRLSLECELLNPLGLFEMKVARKKRRSVDWHHFSVA
AGLAIGGIQWLENKHEAHH

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=344994 EWA64_RS07230 WP_061895829.1 1363922..1364941(-) (pilM) [Vibrio cidicii strain 2423-01]
ATGGGTAAACCCTTAGTTACCGGGATTGATATTGGTCATCGTAGCCTCAAGGCTGTCATTCTGAAACCCGCAGGAAATAG
CTATGCCTTGCTGGGATATAAAGAAATTGTATTTGAGCGCGCTATTGTCGCCGAAAACCACACTCTGAATCATCAGGAAA
TTGTCAATACGCTTAAAGTCCTGAATAAAGAGTTGCCGCGTTTCCGTCGTCAGGTGGCGCTGGCCGTGCCGGATAGTGCC
GTGATCAGCAAGCAATTGCAAATTGAACCGATAGAAGATGAGAAAGAGTGTGAAATCGCCATTTTGCAGGCTTTTTCCCA
TCAATCGCCGTTTCCGGTCGATGAGCTGAATATGGATTATGTGCGTTTGCATGAACCCGCGCAGAGCAAAAGCGCCAGTC
AGAGCGTGCAGATCTTTGCCACTAAGAAAGAGGTGGTCGAGAGTCGGGTGCGCGCCGCGCAGGCGGTGAGGTTGAAACCG
GTGTTGGTGGACATGCACGCCCACTGCTTGTTTAACCTGCGAGAACTGGCGGCGCAGCGCTACCCAGACAAAAGTGCTTT
TTGTTTGTTGGATGTGGGTGCGCAAACCACTTCGCTGATAATTCATCAGCGCGAGAAAACGCACTTTATCAAAGAGTTCG
CGTACGGCGTTGAGCATTTGAACGCCGCGATCGGTGATGAGTTCAGTGCCCATGATGCGCAGGAGAAGACCGAGCGCTTT
ATTGTGGAGATGGTGGATCGCGTCGCGCGACAGTTGCAGCTCTTTCAGTCCATCAATAGTCAAGACAAGCCACAAGGTCT
TTGGTTGTGTGGCGAGGGCGCGGCGACTCCCCTGTTACTCGAAGCGCTCTCTCGTCGTTTGTCGTTGGAGTGTGAACTGC
TCAATCCGCTCGGCCTGTTTGAGATGAAAGTGGCGCGCAAAAAACGTCGCAGTGTTGACTGGCACCACTTTTCGGTTGCC
GCTGGATTAGCCATCGGTGGGATTCAATGGTTGGAGAACAAACATGAAGCACACCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-325)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

54.867

100

0.549
  pilM Vibrio cholerae strain A1552

47.536

100

0.484

Multiple sequence alignment