Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   EWA64_RS07220 Genome accession   NZ_CP035921
Coordinates   1362768..1363358 (-) Length   196 a.a.
NCBI ID   WP_061895831.1    Uniprot ID   -
Organism   Vibrio cidicii strain 2423-01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1357768..1368358
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EWA64_RS07200 aroB 1358608..1359699 (-) 1092 WP_061895833.1 3-dehydroquinate synthase -
  EWA64_RS07205 aroK 1359732..1360250 (-) 519 WP_039423021.1 shikimate kinase AroK -
  EWA64_RS07210 pilQ 1360460..1362235 (-) 1776 WP_061895832.1 type IV pilus secretin PilQ Machinery gene
  EWA64_RS07215 pilP 1362266..1362778 (-) 513 WP_039438951.1 pilus assembly protein PilP Machinery gene
  EWA64_RS07220 pilO 1362768..1363358 (-) 591 WP_061895831.1 type 4a pilus biogenesis protein PilO Machinery gene
  EWA64_RS07225 pilN 1363351..1363938 (-) 588 WP_061895830.1 PilN domain-containing protein Machinery gene
  EWA64_RS07230 pilM 1363922..1364941 (-) 1020 WP_061895829.1 type IV pilus biogenesis protein PilM Machinery gene
  EWA64_RS07235 - 1365080..1367596 (+) 2517 WP_061895828.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22713.90 Da        Isoelectric Point: 4.2450

>NTDB_id=344992 EWA64_RS07220 WP_061895831.1 1362768..1363358(-) (pilO) [Vibrio cidicii strain 2423-01]
MVDWQDLDIDEIAEWPLLPQSLVVLLLMMIIQGFGYWFYLQPEQEALQRAVEEEQNLKVALRIKANKAAALPQLQSQLDE
LSNRYDYLLQQLPAQKELATLLASVNELGLENDLTFTRIDWGEKLSKDFLYQLPLNIELTGDYNDIGQFSQAIAELPRII
NFSDVTWQRVSLESSTLHFRVKANTYQFKPEVAHEK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=344992 EWA64_RS07220 WP_061895831.1 1362768..1363358(-) (pilO) [Vibrio cidicii strain 2423-01]
ATGGTTGATTGGCAGGATCTTGATATCGACGAAATTGCGGAGTGGCCATTGTTGCCACAAAGCTTGGTGGTGCTGCTACT
GATGATGATCATTCAAGGCTTTGGCTACTGGTTCTATCTGCAACCAGAGCAAGAGGCGTTGCAACGGGCGGTGGAGGAGG
AGCAAAACCTCAAAGTCGCACTGCGCATCAAAGCCAACAAAGCGGCCGCCTTGCCTCAGCTTCAGTCTCAGTTGGATGAA
TTATCAAATCGTTACGACTATTTGCTCCAGCAACTGCCTGCGCAAAAAGAGCTGGCGACCTTGCTCGCGTCGGTCAATGA
ACTTGGGTTGGAAAACGATCTCACCTTTACCCGCATCGATTGGGGGGAAAAACTGAGCAAGGATTTCCTCTATCAGCTTC
CTCTCAACATTGAACTGACCGGGGATTACAACGACATCGGCCAGTTTTCCCAAGCGATCGCCGAGCTGCCGCGCATCATT
AACTTTAGCGACGTTACTTGGCAGCGGGTCAGTTTGGAGAGCAGCACACTGCATTTTCGCGTTAAAGCCAACACGTACCA
GTTTAAGCCGGAGGTGGCCCATGAAAAGTAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

76.316

96.939

0.74
  pilO Vibrio cholerae strain A1552

68.878

100

0.689

Multiple sequence alignment