Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   GC226_RS20135 Genome accession   NZ_CP045209
Coordinates   4147928..4148587 (+) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 4     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 4142928..4153587
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GC226_RS20115 (GC226_20285) plsC 4143075..4143812 (-) 738 WP_000965717.1 1-acylglycerol-3-phosphate O-acyltransferase -
  GC226_RS20120 (GC226_20290) parC 4144045..4146303 (-) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  GC226_RS20125 (GC226_20295) ygiV 4146849..4147331 (-) 483 WP_000183494.1 AraC family transcriptional regulator -
  GC226_RS20130 (GC226_20300) ygiW 4147384..4147776 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  GC226_RS20135 (GC226_20305) ciaR 4147928..4148587 (+) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  GC226_RS20140 (GC226_20310) qseC 4148584..4149933 (+) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  GC226_RS20145 (GC226_20315) ygiZ 4149979..4150311 (-) 333 WP_000912123.1 DUF2645 family protein -
  GC226_RS20150 (GC226_20320) mdaB 4150630..4151211 (+) 582 WP_000065440.1 NADPH:quinone oxidoreductase MdaB -
  GC226_RS20155 (GC226_20325) ygiN 4151242..4151556 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  GC226_RS20160 (GC226_20330) - 4151600..4152931 (-) 1332 WP_000778880.1 MFS transporter -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=343958 GC226_RS20135 WP_001221502.1 4147928..4148587(+) (ciaR) [Escherichia coli strain 4]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=343958 GC226_RS20135 WP_001221502.1 4147928..4148587(+) (ciaR) [Escherichia coli strain 4]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment