Detailed information NTG0343958

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: EPB54_RS14235
• Genome accession: NZ_CP035701
• Coordinates: 2838814..2839263 (+)
• Length: 149 a.a.
• NCBI ID: WP_031778455.1
• Uniprot ID: -
• Organism: Vibrio parahaemolyticus strain 2012AW-0154
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2833814..2844263

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EPB54_RS14220	pdhR	2835845..2836612 (-)	768	WP_005462576.1	pyruvate dehydrogenase complex transcriptional repressor PdhR	-
EPB54_RS14225	ampD	2837018..2837569 (-)	552	WP_029855964.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
EPB54_RS14230	nadC	2837662..2838549 (+)	888	WP_015297249.1	carboxylating nicotinate-nucleotide diphosphorylase	-
EPB54_RS14235	pilA	2838814..2839263 (+)	450	WP_031778455.1	pilin	Machinery gene
EPB54_RS14240	pilB	2839267..2840952 (+)	1686	WP_062905453.1	type IV-A pilus assembly ATPase PilB	Machinery gene
EPB54_RS14245	pilC	2840976..2842199 (+)	1224	WP_005479682.1	type II secretion system F family protein	Machinery gene
EPB54_RS14250	pilD	2842264..2843133 (+)	870	WP_031778454.1	A24 family peptidase	Machinery gene
EPB54_RS14255	coaE	2843134..2843748 (+)	615	WP_031778453.1	dephospho-CoA kinase	-

Sequence

Protein

• Length: 149 a.a.
• Molecular weight: 15612.79 Da
• Isoelectric Point: 7.8194

>NTDB_id=343958 EPB54_RS14235 WP_031778455.1 2838814..2839263(+) (pilA) [Vibrio parahaemolyticus strain 2012AW-0154]
MKHSKQKKQQGFTLIELMIVVAVIGVLAAIAIPQYQNYVAKSELGAGLATITSVRTNVEDYIVTNGSFPDGTTAGQLTTD
LGVIQPNNGTITFDDANSNILLTFAGSGNSPDVNNAKIALNRAAGGTWTCKATLDAKLLPKSCSKDANL

Nucleotide

• Length: 450 bp

>NTDB_id=343958 EPB54_RS14235 WP_031778455.1 2838814..2839263(+) (pilA) [Vibrio parahaemolyticus strain 2012AW-0154]
ATGAAACACAGTAAACAGAAAAAACAACAAGGTTTTACCTTGATTGAATTGATGATTGTAGTGGCGGTAATTGGTGTTTT
GGCGGCGATTGCTATCCCTCAATACCAAAACTATGTGGCCAAGTCAGAGTTGGGGGCTGGACTAGCAACAATAACCTCAG
TTCGAACTAATGTTGAAGATTACATTGTTACAAATGGTAGTTTTCCTGATGGCACAACTGCTGGCCAGCTAACAACAGAC
TTAGGAGTGATTCAGCCAAACAATGGTACAATAACTTTTGATGACGCCAATAGCAACATCCTGTTAACTTTTGCTGGCTC
TGGAAATAGCCCTGACGTAAATAATGCAAAAATAGCACTAAACAGAGCGGCTGGTGGTACATGGACATGTAAAGCCACCC
TAGATGCTAAACTACTTCCAAAATCATGTTCTAAGGATGCTAATTTGTAG

Domains

Predicted by InterproScan.

• Pilin (bacterial filament) (Pilin) (40-143)

• Prokaryotic N-terminal methylation motif (N_methyl) (7-31)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	52.288	100	0.537
pilA	Vibrio cholerae strain A1552	52.288	100	0.537
pilA	Vibrio cholerae C6706	52.288	100	0.537
pilA	Pseudomonas aeruginosa PAK	46.309	100	0.463
pilA	Vibrio parahaemolyticus RIMD 2210633	47.059	91.275	0.43
pilA/pilAI	Pseudomonas stutzeri DSM 10701	42.361	96.644	0.409
pilA	Haemophilus influenzae Rd KW20	35.948	100	0.369
pilA2	Legionella pneumophila str. Paris	39.855	92.617	0.369
pilA	Acinetobacter baumannii strain A118	39.706	91.275	0.362
pilA/pilA1	Eikenella corrodens VA1	33.333	100	0.362
pilA2	Legionella pneumophila strain ERS1305867	39.13	92.617	0.362