Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   EPB59_RS00415 Genome accession   NZ_CP035686
Coordinates   89562..91277 (+) Length   571 a.a.
NCBI ID   WP_055033430.1    Uniprot ID   -
Organism   Vibrio metoecus strain 2011V-1169     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 84562..96277
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EPB59_RS00395 pilM 86861..87877 (+) 1017 WP_055029205.1 type IV pilus assembly protein PilM Machinery gene
  EPB59_RS00400 pilN 87867..88445 (+) 579 WP_055029206.1 PilN domain-containing protein Machinery gene
  EPB59_RS00405 pilO 88438..89028 (+) 591 WP_000135851.1 type 4a pilus biogenesis protein PilO Machinery gene
  EPB59_RS00410 pilP 89018..89536 (+) 519 WP_000794742.1 pilus assembly protein PilP Machinery gene
  EPB59_RS00415 pilQ 89562..91277 (+) 1716 WP_055033430.1 type IV pilus secretin PilQ family protein Machinery gene
  EPB59_RS00420 aroK 91464..91988 (+) 525 WP_000818614.1 shikimate kinase AroK -
  EPB59_RS00425 aroB 92016..93101 (+) 1086 WP_195707019.1 3-dehydroquinate synthase -
  EPB59_RS00430 - 93155..94633 (+) 1479 WP_055051281.1 AAA family ATPase -
  EPB59_RS00435 - 94703..95536 (+) 834 WP_154171301.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62337.39 Da        Isoelectric Point: 4.6569

>NTDB_id=343634 EPB59_RS00415 WP_055033430.1 89562..91277(+) (pilQ) [Vibrio metoecus strain 2011V-1169]
MRNGLKTYVAQAWLTLWVGLVLCAASVAHSAESASTNQLQNIDFRVNKDKAAVLIVELASPSVVVDVQKVQEGLSIELLK
TDVADDKLYLLDVKDFSTPVQSIEVFRKEPSTQLIATIDGEFKHDYSLKGKYLEVVISKLKADEKPQPKSILEKEGKLIS
INFQDIPVRNVLQLIADYNGFNLVVSDSVVGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILIAPKDELDLREKQAL
EKARLAEELGDLKSEIIKINFAKASDIAAMIGGEGNVNMLSERGSISIDERTNSLLIRELPDNIAVIREIIESLDIPVKQ
VQIEARIVTVKEGNLDELGVRWGVMSTNGSHSVGGSIESNLWQQGLLADDEFPVDQFLNVNLASASANASSIAFQVAKLG
SDTLLDLELSALQNESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGASTVSFKKAVLSLKVTPQITPDNRLVLDL
SVTQDRRGETVKTGTGEAVSIDTQRIGTQVLVNNGETVVLGGIFQHSINNSVDKVPLLGDLPVLGALFRRTYEQMGKSEL
LIFVTPKVVIQ

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=343634 EPB59_RS00415 WP_055033430.1 89562..91277(+) (pilQ) [Vibrio metoecus strain 2011V-1169]
ATGAGAAATGGATTGAAAACTTATGTCGCACAGGCTTGGCTGACCTTATGGGTTGGTTTGGTGCTTTGTGCTGCTTCCGT
AGCGCATAGCGCGGAGTCGGCTTCTACCAATCAACTGCAAAATATTGATTTTCGGGTCAATAAAGACAAAGCCGCAGTGC
TGATTGTGGAGTTAGCTTCCCCCTCTGTTGTCGTGGATGTTCAAAAAGTCCAAGAAGGGCTGAGTATTGAGCTGCTAAAA
ACGGATGTGGCAGATGACAAGCTTTACTTATTGGATGTAAAGGACTTTTCCACTCCTGTTCAAAGTATAGAAGTGTTCCG
CAAAGAACCTAGCACTCAGTTGATTGCGACCATTGATGGTGAGTTTAAACATGACTACAGCTTGAAAGGAAAATACTTGG
AGGTGGTGATCAGTAAGCTTAAAGCAGATGAAAAACCACAGCCGAAAAGTATTCTAGAGAAAGAAGGCAAGCTGATTTCA
ATTAACTTCCAAGATATTCCTGTGCGTAACGTACTGCAGCTAATTGCCGATTATAACGGCTTCAACTTAGTGGTATCGGA
CTCGGTGGTCGGTAACCTGACGTTACGCTTGGATGGGGTGCCTTGGCAGCAAGTTCTCGACATTATCTTGCAAGTTAAGG
GCTTGGATAAGCGTGTTGATGGTAACGTTATTCTCATTGCACCCAAAGACGAGTTGGATCTACGAGAAAAGCAGGCTTTA
GAGAAAGCGCGTTTAGCGGAAGAACTGGGTGATTTAAAATCAGAAATCATCAAAATCAATTTTGCTAAAGCTTCAGATAT
CGCCGCCATGATTGGCGGAGAAGGCAACGTCAATATGCTCTCTGAGCGGGGATCGATCAGCATTGATGAGCGTACTAACT
CGCTACTGATCCGAGAGTTACCCGATAATATTGCCGTGATCCGCGAGATCATCGAATCGCTGGATATTCCGGTCAAGCAA
GTGCAAATCGAAGCACGTATAGTTACTGTCAAAGAGGGTAACTTGGATGAGCTTGGTGTGCGTTGGGGGGTGATGTCAAC
CAACGGTAGCCATTCCGTTGGTGGAAGTATTGAAAGCAACTTATGGCAACAAGGACTTTTGGCTGACGACGAATTTCCGG
TCGATCAGTTCTTAAATGTGAACTTGGCGTCAGCATCAGCGAACGCTTCCTCTATCGCTTTCCAAGTGGCTAAGCTTGGT
TCCGATACCTTATTGGATTTAGAGCTCTCAGCACTCCAAAACGAGTCGAAAGCAGAAATCATTTCTAGCCCTCGTTTGAT
TACCACTAATAAACAACCAGCGTACATCGAGCAGGGTACGGAAATCCCATACCTTGAGTCATCATCGAGTGGAGCGTCTA
CCGTATCGTTTAAAAAAGCGGTATTGAGCTTGAAAGTTACCCCGCAAATTACGCCGGATAACCGCTTGGTGCTGGATTTG
AGTGTGACTCAAGACCGCCGCGGTGAAACGGTGAAAACCGGTACGGGTGAAGCGGTATCGATCGATACTCAGCGTATTGG
CACACAAGTGTTAGTGAATAATGGAGAAACAGTCGTATTAGGTGGCATTTTCCAGCACAGCATCAATAATTCAGTGGATA
AGGTGCCGCTGCTTGGAGACCTGCCAGTTTTAGGTGCGCTATTTAGACGAACGTACGAGCAAATGGGCAAAAGTGAGCTG
TTGATTTTCGTCACGCCGAAAGTGGTCATTCAATAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(411-569)

Bacterial type II/III secretion system short domain (Secretin_N)

(255-320)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

95.096

100

0.951
  pilQ Vibrio cholerae strain A1552

95.096

100

0.951
  pilQ Vibrio campbellii strain DS40M4

78.909

96.322

0.76
  pilQ Legionella pneumophila strain Lp02

42.989

94.921

0.408
  pilQ Legionella pneumophila strain ERS1305867

42.804

94.921

0.406
  comQ Acinetobacter baylyi ADP1

39.446

100

0.399
  pilQ Acinetobacter baumannii D1279779

38.055

100

0.391
  pilQ Acinetobacter baumannii strain A118

38.055

100

0.391
  pilQ Pseudomonas aeruginosa PAK

38.879

96.848

0.377

Multiple sequence alignment