Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   EPB59_RS00395 Genome accession   NZ_CP035686
Coordinates   86861..87877 (+) Length   338 a.a.
NCBI ID   WP_055029205.1    Uniprot ID   A0A067BAL8
Organism   Vibrio metoecus strain 2011V-1169     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 81861..92877
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EPB59_RS00380 - 82404..83135 (-) 732 WP_055029202.1 glutathione peroxidase -
  EPB59_RS00385 oxyR 83276..84169 (+) 894 WP_055029203.1 DNA-binding transcriptional regulator OxyR -
  EPB59_RS00390 - 84243..86719 (-) 2477 Protein_77 penicillin-binding protein 1A -
  EPB59_RS00395 pilM 86861..87877 (+) 1017 WP_055029205.1 type IV pilus assembly protein PilM Machinery gene
  EPB59_RS00400 pilN 87867..88445 (+) 579 WP_055029206.1 PilN domain-containing protein Machinery gene
  EPB59_RS00405 pilO 88438..89028 (+) 591 WP_000135851.1 type 4a pilus biogenesis protein PilO Machinery gene
  EPB59_RS00410 pilP 89018..89536 (+) 519 WP_000794742.1 pilus assembly protein PilP Machinery gene
  EPB59_RS00415 pilQ 89562..91277 (+) 1716 WP_055033430.1 type IV pilus secretin PilQ family protein Machinery gene
  EPB59_RS00420 aroK 91464..91988 (+) 525 WP_000818614.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37707.55 Da        Isoelectric Point: 7.2213

>NTDB_id=343630 EPB59_RS00395 WP_055029205.1 86861..87877(+) (pilM) [Vibrio metoecus strain 2011V-1169]
MGKSLVTGIDIGHHSIKAVVLKPMGDTYALMGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSQKVAIAIPDNA
VISKVLQIDSDLELREQEFAIYQAFSHQSPFPVEDLSLDFVKVAEKNLARSSTTTFQVYATKKEVVESRLQASRKAGFEP
ILMDVQVHSLLHLWQLASRAYRRADWMLIDIGHTQSSLCLDFPDKMPFYKDVPLGTRLVDLESANISMLNPPQLQDSSQR
FINELVDKVARQIQLFTSVHGAQSLNGLWLSGGGAAMEGLPEALSQRLSLPCEVLNPFALFKMQVSKRKRQVVDGQRFST
AAGLALRGLAWLESEHAA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=343630 EPB59_RS00395 WP_055029205.1 86861..87877(+) (pilM) [Vibrio metoecus strain 2011V-1169]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCCGTGGTGCTCAAACCTATGGGCGACAC
CTATGCGCTTATGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAAAAAGGGGTTGCCTCTATTTAGTCAGAAAGTCGCGATTGCAATCCCGGATAACGCG
GTAATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCTGCGAGAGCAGGAGTTCGCTATTTATCAGGCTTTTTCTCA
TCAATCTCCATTTCCCGTTGAAGATCTTAGCCTTGATTTCGTCAAAGTGGCGGAAAAAAATCTTGCACGCAGTAGTACGA
CGACGTTTCAAGTCTATGCCACCAAGAAAGAAGTGGTTGAAAGCCGCTTGCAAGCCAGTAGGAAAGCAGGTTTTGAGCCG
ATCTTAATGGATGTGCAAGTACACAGTTTGCTTCATTTGTGGCAGTTGGCTAGCCGCGCGTATCGCCGTGCTGACTGGAT
GCTAATTGATATTGGACACACACAAAGCTCTTTGTGCCTGGATTTTCCAGACAAAATGCCTTTTTACAAAGATGTGCCAC
TAGGAACTCGATTGGTCGATTTAGAATCTGCCAACATATCCATGCTCAATCCTCCCCAATTGCAGGACTCATCGCAGCGT
TTTATCAATGAATTGGTGGATAAAGTGGCGCGTCAAATCCAACTGTTTACGTCGGTGCATGGAGCACAAAGCCTCAATGG
TTTGTGGCTATCAGGCGGTGGTGCGGCGATGGAAGGATTGCCAGAAGCGTTGTCCCAGCGTTTATCTTTGCCTTGTGAAG
TGTTGAATCCTTTTGCGTTGTTCAAAATGCAGGTCTCAAAACGTAAACGCCAAGTCGTTGATGGCCAACGTTTTAGCACT
GCTGCAGGACTCGCTTTGCGTGGTTTAGCATGGTTGGAGAGTGAACATGCTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-200)

Type IV pilus assembly protein PilM; (PilM_2)

(241-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A067BAL8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

84.615

100

0.846
  pilM Vibrio campbellii strain DS40M4

48.817

100

0.488

Multiple sequence alignment