Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   EPB59_RS00400 Genome accession   NZ_CP035686
Coordinates   87867..88445 (+) Length   192 a.a.
NCBI ID   WP_055029206.1    Uniprot ID   -
Organism   Vibrio metoecus strain 2011V-1169     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 82867..93445
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EPB59_RS00385 oxyR 83276..84169 (+) 894 WP_055029203.1 DNA-binding transcriptional regulator OxyR -
  EPB59_RS00390 - 84243..86719 (-) 2477 Protein_77 penicillin-binding protein 1A -
  EPB59_RS00395 pilM 86861..87877 (+) 1017 WP_055029205.1 type IV pilus assembly protein PilM Machinery gene
  EPB59_RS00400 pilN 87867..88445 (+) 579 WP_055029206.1 PilN domain-containing protein Machinery gene
  EPB59_RS00405 pilO 88438..89028 (+) 591 WP_000135851.1 type 4a pilus biogenesis protein PilO Machinery gene
  EPB59_RS00410 pilP 89018..89536 (+) 519 WP_000794742.1 pilus assembly protein PilP Machinery gene
  EPB59_RS00415 pilQ 89562..91277 (+) 1716 WP_055033430.1 type IV pilus secretin PilQ family protein Machinery gene
  EPB59_RS00420 aroK 91464..91988 (+) 525 WP_000818614.1 shikimate kinase AroK -
  EPB59_RS00425 aroB 92016..93101 (+) 1086 WP_195707019.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 192 a.a.        Molecular weight: 21925.25 Da        Isoelectric Point: 9.7288

>NTDB_id=343631 EPB59_RS00400 WP_055029206.1 87867..88445(+) (pilN) [Vibrio metoecus strain 2011V-1169]
MLHNVNLLPWRDERREAHKRRFLGLVVLGVLLAVLMQFGAGEYLSGQVAQQQERIGYLKQHIFSLDQQIAKLKVTEEEHK
ALLTRLNVVEQLQQKRNKTTDFMNQMPMLIPEGVYVDKIKMNGHEIEITGISDSTARLATMLDNLEKSPKLSDVEMHEIV
SGNKRFGKQFQSFKVSFQFLRSDSTQGGANNG

Nucleotide


Download         Length: 579 bp        

>NTDB_id=343631 EPB59_RS00400 WP_055029206.1 87867..88445(+) (pilN) [Vibrio metoecus strain 2011V-1169]
ATGCTGCATAACGTTAACTTATTGCCATGGCGAGATGAGCGGCGCGAAGCGCATAAACGGCGCTTTCTGGGGCTTGTGGT
TTTGGGCGTGTTGTTGGCGGTACTGATGCAGTTTGGTGCTGGAGAATATTTAAGTGGTCAGGTCGCGCAACAACAGGAAC
GGATTGGTTATTTGAAACAACACATCTTTTCGTTGGATCAGCAAATCGCCAAACTCAAGGTGACGGAAGAGGAACACAAG
GCATTGTTGACTCGCTTGAATGTTGTTGAGCAGCTACAGCAAAAACGCAATAAAACCACGGACTTCATGAATCAGATGCC
AATGCTGATCCCTGAAGGCGTGTACGTCGATAAGATCAAAATGAATGGGCATGAAATCGAGATAACGGGGATCAGTGACT
CTACAGCTCGCTTAGCGACCATGCTCGACAACTTAGAAAAATCCCCCAAGCTCAGTGACGTAGAGATGCATGAAATTGTC
TCGGGTAACAAACGCTTTGGTAAGCAATTTCAGAGCTTCAAGGTTTCCTTTCAGTTCTTGAGGTCAGACTCGACACAAGG
TGGGGCGAACAATGGCTAG

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

87.113

100

0.88
  pilN Vibrio campbellii strain DS40M4

58.333

100

0.583

Multiple sequence alignment