Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   ETT50_RS03275 Genome accession   NZ_CP035449
Coordinates   625288..625713 (+) Length   141 a.a.
NCBI ID   WP_011285575.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain emm56     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 613819..658426 625288..625713 within 0


Gene organization within MGE regions


Location: 613819..658426
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ETT50_RS03180 (ETT50_03180) - 613837..614679 (+) 843 WP_002992620.1 SGNH/GDSL hydrolase family protein -
  ETT50_RS03185 (ETT50_03185) - 614657..615244 (+) 588 WP_010922482.1 YpmS family protein -
  ETT50_RS03190 (ETT50_03190) - 615343..615618 (+) 276 WP_002983920.1 HU family DNA-binding protein -
  ETT50_RS03195 (ETT50_03195) - 615707..616849 (-) 1143 WP_032465117.1 site-specific integrase -
  ETT50_RS03200 (ETT50_03200) - 616974..617279 (-) 306 WP_011106738.1 membrane protein -
  ETT50_RS03205 (ETT50_03205) - 617289..618077 (-) 789 WP_030127591.1 S24 family peptidase -
  ETT50_RS09390 - 618447..618596 (+) 150 WP_021340643.1 hypothetical protein -
  ETT50_RS03210 (ETT50_03210) - 618582..618797 (-) 216 WP_014635530.1 hypothetical protein -
  ETT50_RS03215 (ETT50_03215) - 618856..619014 (+) 159 WP_021340638.1 hypothetical protein -
  ETT50_RS03220 (ETT50_03220) - 619113..619754 (-) 642 WP_001008979.1 hypothetical protein -
  ETT50_RS03225 (ETT50_03225) - 619847..620086 (+) 240 WP_014411882.1 helix-turn-helix transcriptional regulator -
  ETT50_RS03230 (ETT50_03230) - 620097..620858 (+) 762 WP_023610918.1 phage antirepressor KilAC domain-containing protein -
  ETT50_RS09510 - 620871..621002 (+) 132 WP_002985401.1 hypothetical protein -
  ETT50_RS03235 (ETT50_03235) - 620999..621181 (-) 183 WP_011889039.1 hypothetical protein -
  ETT50_RS03240 (ETT50_03240) - 621287..621598 (+) 312 WP_032465116.1 hypothetical protein -
  ETT50_RS09515 - 621600..621734 (+) 135 WP_261729371.1 hypothetical protein -
  ETT50_RS03245 (ETT50_03245) - 621856..622617 (+) 762 WP_014635613.1 DnaD domain protein -
  ETT50_RS03250 (ETT50_03250) - 622604..623386 (+) 783 WP_032465115.1 ATP-binding protein -
  ETT50_RS09395 - 623377..623514 (+) 138 WP_011018145.1 hypothetical protein -
  ETT50_RS03255 (ETT50_03255) - 623525..623881 (+) 357 WP_021341157.1 HTH domain-containing protein -
  ETT50_RS03260 (ETT50_03260) - 623862..624116 (+) 255 WP_011018143.1 hypothetical protein -
  ETT50_RS03265 (ETT50_03265) - 624138..624620 (+) 483 WP_011018142.1 siphovirus Gp157 family protein -
  ETT50_RS03270 (ETT50_03270) - 624621..625295 (+) 675 WP_032465114.1 ERF family protein -
  ETT50_RS03275 (ETT50_03275) ssb 625288..625713 (+) 426 WP_011285575.1 single-stranded DNA-binding protein Machinery gene
  ETT50_RS03280 (ETT50_03280) - 625719..625922 (+) 204 WP_032465113.1 hypothetical protein -
  ETT50_RS03285 (ETT50_03285) - 625922..626362 (+) 441 WP_032465112.1 RusA family crossover junction endodeoxyribonuclease -
  ETT50_RS03290 (ETT50_03290) - 626359..626715 (+) 357 WP_011284873.1 hypothetical protein -
  ETT50_RS09650 (ETT50_03295) - 626712..626957 (+) 246 WP_011285573.1 hypothetical protein -
  ETT50_RS03300 (ETT50_03300) - 626957..627169 (+) 213 WP_032465111.1 DUF3310 domain-containing protein -
  ETT50_RS09400 - 627190..627360 (+) 171 WP_002995952.1 hypothetical protein -
  ETT50_RS03305 (ETT50_03305) - 627357..627641 (+) 285 WP_011284869.1 hypothetical protein -
  ETT50_RS03310 (ETT50_03310) - 627643..628275 (+) 633 WP_011018133.1 N-6 DNA methylase -
  ETT50_RS03315 (ETT50_03315) - 628651..629085 (+) 435 WP_032461637.1 ArpU family phage packaging/lysis transcriptional regulator -
  ETT50_RS03340 (ETT50_03340) - 630018..630932 (+) 915 WP_011284864.1 hypothetical protein -
  ETT50_RS09405 - 631022..631255 (-) 234 WP_002995461.1 hypothetical protein -
  ETT50_RS03350 (ETT50_03350) - 631550..631927 (-) 378 WP_002987543.1 type II toxin-antitoxin system HicB family antitoxin -
  ETT50_RS03355 (ETT50_03355) - 631979..632164 (-) 186 WP_032465109.1 type II toxin-antitoxin system HicA family toxin -
  ETT50_RS03365 (ETT50_03365) - 632400..632741 (+) 342 WP_029714359.1 HNH endonuclease -
  ETT50_RS03370 (ETT50_03370) - 632910..633377 (+) 468 WP_002985371.1 phage terminase small subunit P27 family -
  ETT50_RS03375 (ETT50_03375) - 633392..635146 (+) 1755 WP_032465108.1 terminase large subunit -
  ETT50_RS09410 - 635143..635313 (+) 171 WP_002985365.1 hypothetical protein -
  ETT50_RS03380 (ETT50_03380) - 635306..635530 (+) 225 WP_002985363.1 hypothetical protein -
  ETT50_RS03385 (ETT50_03385) - 635564..636784 (+) 1221 WP_032465107.1 phage portal protein -
  ETT50_RS03390 (ETT50_03390) - 636762..637427 (+) 666 WP_032465106.1 head maturation protease, ClpP-related -
  ETT50_RS03395 (ETT50_03395) - 637452..638636 (+) 1185 WP_021340812.1 phage major capsid protein -
  ETT50_RS09520 - 638650..638778 (+) 129 WP_021340813.1 hypothetical protein -
  ETT50_RS03400 (ETT50_03400) - 638781..639083 (+) 303 WP_021340811.1 head-tail connector protein -
  ETT50_RS03405 (ETT50_03405) - 639080..639427 (+) 348 WP_003055344.1 phage head closure protein -
  ETT50_RS03410 (ETT50_03410) - 639424..639801 (+) 378 WP_003055321.1 HK97-gp10 family putative phage morphogenesis protein -
  ETT50_RS03415 (ETT50_03415) - 639798..640223 (+) 426 WP_011017584.1 hypothetical protein -
  ETT50_RS03420 (ETT50_03420) - 640239..640847 (+) 609 WP_011017585.1 major tail protein -
  ETT50_RS03425 (ETT50_03425) gpG 640900..641226 (+) 327 WP_011017586.1 phage tail assembly chaperone G -
  ETT50_RS09415 - 641274..641423 (+) 150 WP_021299462.1 hypothetical protein -
  ETT50_RS03430 (ETT50_03430) - 641436..645359 (+) 3924 WP_032465105.1 phage tail tape measure protein -
  ETT50_RS03435 (ETT50_03435) - 645359..646066 (+) 708 WP_032465104.1 distal tail protein Dit -
  ETT50_RS03440 (ETT50_03440) - 646063..648207 (+) 2145 WP_032465102.1 phage tail spike protein -
  ETT50_RS03445 (ETT50_03445) hylP 648204..649229 (+) 1026 WP_032465101.1 hyaluronidase HylP -
  ETT50_RS03450 (ETT50_03450) - 649242..651125 (+) 1884 WP_136118837.1 gp58-like family protein -
  ETT50_RS03455 (ETT50_03455) - 651137..651568 (+) 432 WP_002987513.1 DUF1617 family protein -
  ETT50_RS03460 (ETT50_03460) - 651571..652188 (+) 618 WP_032461328.1 DUF1366 domain-containing protein -
  ETT50_RS03465 (ETT50_03465) - 652198..652473 (+) 276 WP_002987582.1 hypothetical protein -
  ETT50_RS03470 (ETT50_03470) - 652470..652697 (+) 228 WP_000609113.1 phage holin -
  ETT50_RS03475 (ETT50_03475) - 652813..654015 (+) 1203 WP_032465097.1 glucosaminidase domain-containing protein -
  ETT50_RS09525 (ETT50_03480) - 654048..654230 (+) 183 Protein_612 holin -
  ETT50_RS03485 (ETT50_03485) - 654382..654924 (+) 543 WP_032465096.1 Panacea domain-containing protein -
  ETT50_RS03490 (ETT50_03490) - 654928..655764 (+) 837 WP_032465095.1 hypothetical protein -
  ETT50_RS03495 (ETT50_03495) spek 656068..656847 (+) 780 WP_011054728.1 streptococcal pyrogenic exotoxin SpeK -
  ETT50_RS03500 (ETT50_03500) - 657323..657898 (+) 576 WP_011054727.1 hypothetical protein -
  ETT50_RS03510 (ETT50_03510) prx 658244..658426 (+) 183 WP_032465094.1 Paratox Regulator

Sequence


Protein


Download         Length: 141 a.a.        Molecular weight: 15995.63 Da        Isoelectric Point: 4.5748

>NTDB_id=342186 ETT50_RS03275 WP_011285575.1 625288..625713(+) (ssb) [Streptococcus pyogenes strain emm56]
MINNIVLVGRMTKDAELRYTASQVAVATFTLAVNRRFKEQNGEREADFINCVIWRQSAENLANWAKKGALIGVTGRIQTR
NYENQQEQRVYVTEVVAESFQMLESRNSQQQSGQDNSSQNDNSQPFGNSNPMDISDDDLPF

Nucleotide


Download         Length: 426 bp        

>NTDB_id=342186 ETT50_RS03275 WP_011285575.1 625288..625713(+) (ssb) [Streptococcus pyogenes strain emm56]
ATGATTAACAACATTGTGCTAGTTGGTCGCATGACCAAGGACGCAGAGCTTCGCTATACAGCGAGTCAAGTAGCTGTAGC
TACGTTCACACTTGCGGTAAACCGCAGATTTAAAGAGCAAAACGGGGAGAGAGAAGCAGATTTCATTAACTGTGTTATCT
GGCGACAGTCTGCTGAAAATTTAGCCAACTGGGCTAAAAAAGGTGCTTTGATCGGAGTTACGGGTCGTATTCAGACACGT
AACTACGAAAACCAACAAGAACAACGTGTCTATGTGACGGAAGTTGTTGCAGAGAGTTTCCAAATGTTGGAAAGTCGAAA
TAGCCAGCAACAATCTGGTCAAGATAACTCTTCGCAAAACGATAACAGTCAACCGTTTGGCAATTCAAACCCAATGGATA
TTTCAGACGATGATCTGCCGTTTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

55.294

100

0.667

  ssbA Bacillus subtilis subsp. subtilis str. 168

54.07

100

0.66

  ssbB/cilA Streptococcus pneumoniae TIGR4

43.972

100

0.44

  ssbB/cilA Streptococcus pneumoniae Rx1

43.262

100

0.433

  ssbB Streptococcus sobrinus strain NIDR 6715-7

43.262

100

0.433

  ssbB/cilA Streptococcus mitis NCTC 12261

43.262

100

0.433

  ssbB/cilA Streptococcus pneumoniae D39

43.262

100

0.433

  ssbB/cilA Streptococcus pneumoniae R6

43.262

100

0.433

  ssbB/cilA Streptococcus mitis SK321

43.262

100

0.433

  ssbB Bacillus subtilis subsp. subtilis str. 168

51.786

79.433

0.411

  ssbA Streptococcus mutans UA159

40.426

100

0.404

  ssb Glaesserella parasuis strain SC1401

29.379

100

0.369

  ssbB Lactococcus lactis subsp. cremoris KW2

49.524

74.468

0.369


Multiple sequence alignment