Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   EK374_RS16565 Genome accession   NZ_CP034683
Coordinates   3642816..3644453 (-) Length   545 a.a.
NCBI ID   WP_233280269.1    Uniprot ID   -
Organism   Rheinheimera mangrovi strain LHK132     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3637816..3649453
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EK374_RS16535 - 3637929..3638195 (+) 267 WP_127025650.1 hypothetical protein -
  EK374_RS21165 - 3638197..3638391 (+) 195 WP_127025651.1 DUF2970 domain-containing protein -
  EK374_RS16545 - 3638310..3639209 (-) 900 WP_127025652.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  EK374_RS16550 - 3639246..3641036 (-) 1791 WP_127025653.1 SPOR domain-containing protein -
  EK374_RS16555 aroB 3641033..3642100 (-) 1068 WP_127025654.1 3-dehydroquinate synthase -
  EK374_RS16560 aroK 3642139..3642657 (-) 519 WP_008900467.1 shikimate kinase AroK -
  EK374_RS16565 pilQ 3642816..3644453 (-) 1638 WP_233280269.1 type IV pilus secretin PilQ Machinery gene
  EK374_RS16570 - 3644538..3645083 (-) 546 WP_127025656.1 pilus assembly protein PilP -
  EK374_RS16575 - 3645080..3645679 (-) 600 WP_127025657.1 type 4a pilus biogenesis protein PilO -
  EK374_RS16580 - 3645676..3646290 (-) 615 WP_127025658.1 PilN domain-containing protein -
  EK374_RS16585 - 3646278..3647357 (-) 1080 WP_127025659.1 pilus assembly protein PilM -

Sequence

Protein


Download         Length: 545 a.a.        Molecular weight: 59975.90 Da        Isoelectric Point: 4.6150

>NTDB_id=332462 EK374_RS16565 WP_233280269.1 3642816..3644453(-) (pilQ) [Rheinheimera mangrovi strain LHK132]
MGNVAAQQGSKDFINEIDSIDFRRGEQGEAKVLVFLQQNSAAVDVIEQNGKIQLDFHDTKISDELLYQLDVLDFATVVKG
IETFREGSKARLVIETLDTFTYSYQQIDEVFHLVVQKKSAEQTKSAATGQYVGRAISLNFQDIPVRTVLQIIADYNGFNL
VTSDSVTGNITLRLDGTPWDQALDIVLKVRGLDKRMDGNILMIAPSDELAAREAKELQALQQVEQLEPLMSEFLPINYAK
AADIAALLNNKDATLLSARGNVAVDDRTNTLLVKDTATTIENVRRLLRRLDVPVKQVLIESRMVTVKDNVRDEIGLRWGF
SDQQNDRAWSGTAPGANNLAGGTVPSLTDRWNVNLPVANPAGSIAFHVTKFADGTLLDLELSALEQENKGEIIASPRITT
ANQKKARIEQGTEIPYLEASSSGATTVTFKKAVLSLEVTPQITPDNKIILDLEITQDTRGETVSTGNGPAQAIDTQRIQT
QVLVDNGATVVLGGIYQQQMTNTTSKVPVFGDIPYLGNLFKRRNDFNEKTELLIFVTPKILTESL

Nucleotide


Download         Length: 1638 bp        

>NTDB_id=332462 EK374_RS16565 WP_233280269.1 3642816..3644453(-) (pilQ) [Rheinheimera mangrovi strain LHK132]
ATGGGAAATGTTGCAGCCCAACAAGGCAGCAAGGACTTTATTAATGAAATTGATTCAATAGATTTTCGCCGTGGTGAACA
AGGTGAAGCTAAGGTATTGGTATTTCTGCAACAAAATTCAGCTGCTGTGGATGTCATTGAACAAAATGGCAAAATTCAGC
TGGATTTTCATGACACGAAAATCAGCGATGAGTTGTTGTATCAACTGGATGTGCTGGATTTTGCCACTGTGGTTAAAGGT
ATAGAAACCTTCAGAGAAGGCAGTAAAGCAAGGCTGGTGATTGAAACTCTTGATACCTTCACCTATAGCTACCAGCAAAT
TGACGAGGTCTTTCATTTGGTGGTGCAGAAAAAATCTGCAGAACAAACCAAATCTGCTGCAACTGGCCAGTATGTTGGTA
GGGCTATTTCTTTGAATTTTCAGGATATTCCGGTTCGGACTGTGTTGCAGATTATTGCTGATTACAATGGTTTTAATCTG
GTGACCAGCGATTCAGTGACAGGCAATATCACCTTAAGGCTGGACGGTACGCCTTGGGATCAGGCGCTGGATATAGTGCT
GAAAGTACGTGGGCTGGATAAACGTATGGACGGCAATATTCTGATGATAGCGCCATCCGACGAACTGGCAGCACGGGAAG
CGAAAGAGCTGCAGGCTCTGCAGCAGGTTGAGCAGTTAGAACCTTTGATGTCCGAGTTTTTGCCTATTAACTACGCCAAA
GCTGCGGATATTGCCGCTTTGCTCAATAACAAAGACGCGACCTTATTATCTGCCCGAGGCAATGTGGCAGTGGACGATAG
AACCAACACTTTATTGGTAAAAGATACCGCGACCACGATCGAAAATGTACGGCGTTTGCTGCGTCGGCTGGATGTGCCGG
TAAAACAAGTGCTGATTGAATCCCGTATGGTCACAGTCAAAGACAATGTGCGGGACGAGATTGGTTTACGTTGGGGCTTC
AGTGACCAGCAAAATGACAGGGCCTGGTCTGGTACAGCGCCTGGTGCTAATAATCTGGCGGGCGGTACAGTACCTTCGTT
AACTGACCGATGGAATGTGAACTTACCTGTCGCCAATCCGGCGGGTAGTATAGCTTTTCATGTCACTAAGTTTGCCGATG
GCACTTTGCTGGATTTGGAACTGTCAGCGCTGGAGCAGGAAAACAAGGGTGAAATCATTGCCAGCCCACGTATTACCACG
GCCAATCAGAAAAAAGCCCGGATAGAGCAGGGCACTGAAATTCCTTACCTGGAGGCTTCCAGCAGCGGTGCCACGACCGT
TACTTTTAAAAAGGCGGTATTAAGCCTTGAAGTGACACCACAAATTACGCCGGACAATAAAATTATTCTGGATTTGGAGA
TCACTCAGGACACTCGCGGCGAAACTGTATCGACAGGCAATGGCCCAGCTCAGGCGATAGATACGCAACGTATCCAGACC
CAGGTGCTGGTCGATAATGGCGCTACTGTGGTCTTGGGTGGCATCTACCAGCAACAGATGACCAACACCACCTCAAAAGT
ACCGGTTTTTGGCGACATTCCTTATTTAGGAAATTTGTTTAAACGCCGTAATGACTTTAATGAAAAAACTGAATTATTGA
TTTTCGTCACCCCAAAAATACTGACCGAAAGCTTGTAG

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(160-205)

Bacterial type II/III secretion system short domain (Secretin_N)

(233-296)

Bacterial type II and III secretion system protein (Secretin)

(383-541)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

50.459

100

0.505
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

50.56

98.349

0.497
  pilQ Vibrio cholerae strain A1552

50.56

98.349

0.497
  pilQ Legionella pneumophila strain ERS1305867

46.679

99.45

0.464
  pilQ Legionella pneumophila strain Lp02

46.58

99.266

0.462
  comQ Acinetobacter baylyi ADP1

39.182

100

0.422
  pilQ Acinetobacter baumannii D1279779

38.927

100

0.413
  pilQ Acinetobacter baumannii strain A118

38.927

100

0.413
  pilQ Pseudomonas aeruginosa PAK

40.399

100

0.409
  pilQ Neisseria gonorrhoeae strain FA1090

38.095

100

0.382
  pilQ Neisseria meningitidis MC58

38.235

99.817

0.382
  pilQ Neisseria meningitidis M1080

38.235

99.817

0.382

Multiple sequence alignment