Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   EIY89_RS02210 Genome accession   NZ_CP034246
Coordinates   468042..468488 (-) Length   148 a.a.
NCBI ID   WP_190534345.1    Uniprot ID   -
Organism   Shewanella algae strain VGH117     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 463042..473488
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EIY89_RS02190 (EIY89_02185) coaE 463241..463852 (-) 612 WP_025012056.1 dephospho-CoA kinase -
  EIY89_RS02195 (EIY89_02190) pilD 463853..464767 (-) 915 WP_190534336.1 A24 family peptidase Machinery gene
  EIY89_RS02200 (EIY89_02195) pilC 464837..466105 (-) 1269 WP_190534339.1 type II secretion system F family protein Machinery gene
  EIY89_RS02205 (EIY89_02200) pilB 466266..467972 (-) 1707 WP_190534342.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EIY89_RS02210 (EIY89_02205) pilA/pilAI 468042..468488 (-) 447 WP_190534345.1 pilin Machinery gene
  EIY89_RS02215 (EIY89_02210) nadC 469357..470217 (-) 861 WP_025012052.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EIY89_RS02220 (EIY89_02215) ampD 470376..470954 (+) 579 WP_144159901.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EIY89_RS02225 (EIY89_02220) ampE 471008..471859 (+) 852 WP_025012050.1 beta-lactamase regulator AmpE -
  EIY89_RS02230 (EIY89_02225) pdhR 472134..472886 (+) 753 WP_025886793.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15528.89 Da        Isoelectric Point: 8.4732

>NTDB_id=330187 EIY89_RS02210 WP_190534345.1 468042..468488(-) (pilA/pilAI) [Shewanella algae strain VGH117]
MKGIKLNKRAQGFTLIELMIVVAIIGILAAIALPAYQDYTVKSQAGSAYAEVSGLKTAFEAAVNEGKTPVLTRAVADTTE
AFVGQTADGGTYCTLAITGITKIECTTKGGNAAKFNGKKINMNRDADGVWTCTTDLEDKFKPGNCKAA

Nucleotide


Download         Length: 447 bp        

>NTDB_id=330187 EIY89_RS02210 WP_190534345.1 468042..468488(-) (pilA/pilAI) [Shewanella algae strain VGH117]
ATGAAAGGTATCAAACTTAACAAACGCGCTCAGGGCTTCACCCTGATCGAACTGATGATCGTAGTAGCCATTATCGGTAT
TCTCGCGGCAATCGCACTGCCTGCTTATCAGGATTACACCGTTAAGTCTCAAGCAGGTTCAGCATATGCTGAAGTGTCTG
GATTAAAGACCGCTTTTGAAGCTGCAGTAAACGAAGGAAAAACCCCTGTTTTAACTAGGGCTGTAGCCGATACAACAGAA
GCGTTTGTTGGGCAAACAGCAGACGGTGGAACTTATTGTACGCTTGCCATTACTGGTATTACCAAAATTGAATGTACAAC
CAAAGGTGGTAACGCTGCCAAATTCAATGGTAAGAAAATCAACATGAATCGTGATGCAGACGGCGTATGGACATGCACTA
CTGACCTCGAAGATAAGTTTAAGCCAGGCAACTGTAAAGCTGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(41-146)

Prokaryotic N-terminal methylation motif (N_methyl)

(8-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

59.854

92.568

0.554
  pilA/pilAII Pseudomonas stutzeri DSM 10701

52.381

99.324

0.52
  pilA Acinetobacter baumannii strain A118

47.222

97.297

0.459
  pilA Pseudomonas aeruginosa PAK

44.898

99.324

0.446
  pilA Vibrio cholerae C6706

42.105

100

0.432
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.105

100

0.432
  pilA Vibrio cholerae strain A1552

42.105

100

0.432
  pilE Neisseria gonorrhoeae strain FA1090

33.537

100

0.372
  pilA2 Legionella pneumophila str. Paris

38.462

96.622

0.372
  pilA2 Legionella pneumophila strain ERS1305867

37.762

96.622

0.365

Multiple sequence alignment