Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EIY89_RS02205 Genome accession   NZ_CP034246
Coordinates   466266..467972 (-) Length   568 a.a.
NCBI ID   WP_190534342.1    Uniprot ID   -
Organism   Shewanella algae strain VGH117     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 461266..472972
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EIY89_RS02170 (EIY89_02165) - 461515..461778 (-) 264 Protein_403 transposase -
  EIY89_RS02175 (EIY89_02170) mutT 461849..462247 (-) 399 WP_025012058.1 8-oxo-dGTP diphosphatase MutT -
  EIY89_RS02180 (EIY89_02175) yacG 462247..462459 (-) 213 WP_025886789.1 DNA gyrase inhibitor YacG -
  EIY89_RS02185 (EIY89_02180) zapD 462504..463244 (-) 741 WP_028779562.1 cell division protein ZapD -
  EIY89_RS02190 (EIY89_02185) coaE 463241..463852 (-) 612 WP_025012056.1 dephospho-CoA kinase -
  EIY89_RS02195 (EIY89_02190) pilD 463853..464767 (-) 915 WP_190534336.1 A24 family peptidase Machinery gene
  EIY89_RS02200 (EIY89_02195) pilC 464837..466105 (-) 1269 WP_190534339.1 type II secretion system F family protein Machinery gene
  EIY89_RS02205 (EIY89_02200) pilB 466266..467972 (-) 1707 WP_190534342.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EIY89_RS02210 (EIY89_02205) pilA/pilAI 468042..468488 (-) 447 WP_190534345.1 pilin Machinery gene
  EIY89_RS02215 (EIY89_02210) nadC 469357..470217 (-) 861 WP_025012052.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EIY89_RS02220 (EIY89_02215) ampD 470376..470954 (+) 579 WP_144159901.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EIY89_RS02225 (EIY89_02220) ampE 471008..471859 (+) 852 WP_025012050.1 beta-lactamase regulator AmpE -
  EIY89_RS02230 (EIY89_02225) pdhR 472134..472886 (+) 753 WP_025886793.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63032.39 Da        Isoelectric Point: 5.4776

>NTDB_id=330186 EIY89_RS02205 WP_190534342.1 466266..467972(-) (pilB) [Shewanella algae strain VGH117]
MPTTGLHLGLSALFIRKKLLSEESVAPLILKSRKTNQSLISTIVSEKLLSAREIAELCYEEYGTPLLDLNEFDLSSIPEE
FLNKKLIDKHRCLPLFKRGNRLYIATSDPTNIAALEDFQFSAGLHAEAILVEEDKLSKALEKVLEEDLSSLELDGLDEKS
LAGIEVADTDKRQQEENEGKDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLSGRISAR
LKVMSKLDIAERRVPQDGRIKMKLSRNKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEEDQKQAYLEMLARPQ
GMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSSAETLTRLLNMGVPGYNIASSVNLIIAQRLARRLCPECRQPEDIPEHELLQLGFS
QQQIDAGFTPYKPCGCDLCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIAATAKQQGMRDLRLSGLLKVIQGVTSIA
EVNRVTSY

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=330186 EIY89_RS02205 WP_190534342.1 466266..467972(-) (pilB) [Shewanella algae strain VGH117]
ATGCCTACAACGGGTTTACATCTCGGTCTATCAGCTCTCTTTATTCGCAAAAAACTTCTGAGCGAAGAAAGTGTTGCGCC
ATTAATACTTAAGTCCCGAAAAACCAATCAATCTCTCATTTCAACTATTGTTTCTGAGAAATTGTTATCGGCAAGGGAAA
TTGCAGAACTCTGCTACGAAGAGTACGGCACCCCACTGCTGGATCTCAATGAGTTTGATTTAAGCAGTATCCCGGAAGAG
TTTCTCAATAAAAAACTGATCGACAAGCACAGATGCCTGCCGTTATTCAAGCGCGGCAACCGCCTCTATATCGCCACCTC
AGACCCCACCAATATCGCTGCTCTGGAAGATTTTCAGTTCAGCGCCGGCCTACACGCAGAAGCGATACTGGTCGAAGAGG
ACAAACTAAGCAAGGCACTGGAAAAAGTACTCGAGGAAGACCTCAGCTCTTTAGAGCTAGATGGTCTCGATGAGAAGTCT
CTAGCAGGTATCGAAGTCGCCGATACCGACAAGCGTCAACAGGAAGAGAATGAAGGCAAGGATGACGCCCCTATCGTCAT
CTATATCAATAAGATTTTGACTGATGCCATCCGCAAGGGCGCATCGGATCTGCATTTCGAGCCTTACGAGAAGCGCTACC
GCATCCGCTTTCGTATTGATGGCATCCTCCACGAGGTTTCCGAGCCACCAATTTCCCTCTCGGGCCGCATATCAGCCCGC
TTGAAAGTGATGTCCAAATTGGATATCGCCGAGCGCCGTGTTCCTCAGGATGGCCGCATCAAAATGAAGCTGTCCCGCAA
CAAGAGTATCGATTTCCGGGTCAGTACCCTGCCCACCCTCTGGGGTGAAAAGATAGTGATGCGGATCCTCGACTCATCTT
CCGCACAATTAGGAATCGAAAAACTCGGCTATGAGGAAGACCAAAAACAAGCCTACCTGGAGATGCTGGCAAGACCCCAG
GGGATGATTCTGGTGACAGGCCCTACCGGCTCGGGTAAAACCGTTTCCCTTTATACTGGCCTCAATATCCTCAATACCGA
AGAGCGCAATATCAGCACCGCCGAAGACCCGGTAGAGATAAACCTGGAAGGGGTCAACCAGGTACATATCAACCCCAAGG
CCGGGCTAACCTTCGCCTCGGCGCTGCGCTCCTTTTTGCGTCAAGACCCGGATGTGGTGATGGTGGGGGAAATTCGCGAC
CTGGAAACCGCCGAAATCGCCATTAAGGCGGCGCAAACTGGCCACTTGGTACTCTCGACCCTGCATACCAACTCATCGGC
CGAAACCCTTACTCGCCTGCTTAATATGGGTGTTCCCGGCTATAATATCGCCAGCTCAGTCAACCTGATTATTGCCCAGC
GCCTGGCCCGCCGCCTCTGTCCCGAATGCCGCCAACCGGAAGATATCCCCGAGCATGAACTGCTGCAGCTGGGATTTAGT
CAGCAGCAGATAGACGCAGGCTTTACCCCTTATAAGCCCTGCGGTTGCGATCTCTGCTCCGGAGGCTACAAGGGCCGGGT
CGGTATCTATGAGGTAATGAAGATGTCCGATGAAATCGCCAGAACCATTATGGAAGGCGGCAACTCACTGCAGATTGCCG
CCACCGCCAAGCAGCAAGGGATGCGCGATCTGCGCCTTTCTGGCCTTTTAAAAGTGATTCAAGGTGTCACCAGTATTGCC
GAAGTGAACCGGGTCACCAGCTATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.111

98.768

0.574
  pilB Acinetobacter baylyi ADP1

60

95.07

0.57
  pilB Legionella pneumophila strain ERS1305867

56.714

99.648

0.565
  pilB Vibrio parahaemolyticus RIMD 2210633

52.993

100

0.53
  pilB Vibrio cholerae strain A1552

52.228

98.768

0.516
  pilB Vibrio campbellii strain DS40M4

51.51

99.12

0.511
  pilF Neisseria gonorrhoeae MS11

47.257

99.472

0.47
  pilF Thermus thermophilus HB27

37.809

99.648

0.377

Multiple sequence alignment