Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilE   Type   Machinery gene
Locus tag   FOY95_RS05830 Genome accession   NZ_CP041773
Coordinates   1183301..1183774 (-) Length   157 a.a.
NCBI ID   WP_015503553.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 519119     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 1178301..1188774
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOY95_RS05790 (FOY95_05790) - 1178743..1179405 (+) 663 WP_033992423.1 DUF3218 family protein -
  FOY95_RS05800 (FOY95_05800) fliP 1179685..1180440 (+) 756 WP_031285507.1 flagellar type III secretion system pore protein FliP -
  FOY95_RS05805 (FOY95_05805) fliQ 1180485..1180754 (+) 270 WP_023435365.1 flagellar biosynthesis protein FliQ -
  FOY95_RS05810 (FOY95_05810) fliR 1180754..1181536 (+) 783 Protein_1120 flagellar biosynthetic protein FliR -
  FOY95_RS05815 (FOY95_05815) - 1181539..1181798 (+) 260 Protein_1121 EscU/YscU/HrcU family type III secretion system export apparatus switch protein -
  FOY95_RS05820 (FOY95_05820) - 1182828..1183070 (+) 243 WP_023082027.1 hypothetical protein -
  FOY95_RS05830 (FOY95_05830) pilE 1183301..1183774 (-) 474 WP_015503553.1 pilin Machinery gene
  FOY95_RS05835 (FOY95_05835) pilB 1184005..1185704 (+) 1700 Protein_1124 type IV-A pilus assembly ATPase PilB -
  FOY95_RS05840 (FOY95_05840) pilC 1185708..1186925 (+) 1218 WP_010792214.1 type II secretion system F family protein Machinery gene
  FOY95_RS05845 (FOY95_05845) pilD 1186928..1187800 (+) 873 WP_019726811.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  FOY95_RS05850 (FOY95_05850) coaE 1187797..1188408 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  FOY95_RS05855 (FOY95_05855) yacG 1188405..1188605 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence


Protein


Download         Length: 157 a.a.        Molecular weight: 16090.45 Da        Isoelectric Point: 8.4684

>NTDB_id=328726 FOY95_RS05830 WP_015503553.1 1183301..1183774(-) (pilE) [Pseudomonas aeruginosa strain 519119]
MKAAQKGFTLIELMIVVAIIGILAAIAIPAYQDYTARAQLSEAMTLASGLKTKVSDIFSQDGSCPANTAATAGIEKDTDI
NGKYVAKVTTGGTAAASGGCTIVATMKASDVATPLRGKTLTLTLGNADKGSYTWACTSNADNKYLPKTCQTATTTTP

Nucleotide


Download         Length: 474 bp        

>NTDB_id=328726 FOY95_RS05830 WP_015503553.1 1183301..1183774(-) (pilE) [Pseudomonas aeruginosa strain 519119]
ATGAAAGCTGCTCAAAAAGGCTTTACCTTGATCGAATTGATGATCGTGGTCGCGATCATCGGTATTCTGGCTGCCATCGC
TATTCCAGCGTATCAGGACTACACCGCACGCGCTCAGCTTAGCGAAGCCATGACCCTGGCCAGTGGTCTCAAGACGAAAG
TGAGCGATATCTTCTCTCAGGATGGGTCCTGCCCGGCTAATACTGCTGCCACGGCAGGCATCGAGAAAGATACCGACATC
AACGGCAAGTATGTTGCCAAGGTAACAACTGGTGGCACCGCAGCTGCGTCTGGTGGTTGCACTATCGTTGCTACTATGAA
AGCCTCTGATGTGGCTACTCCTCTGAGGGGGAAAACTCTGACTTTGACTCTAGGAAATGCTGACAAGGGTTCTTACACTT
GGGCCTGTACTTCCAACGCAGATAACAAGTACCTGCCAAAAACCTGCCAGACTGCTACCACTACCACTCCGTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

42.197

100

0.465

  comP Acinetobacter baylyi ADP1

47.059

97.452

0.459

  pilE Neisseria gonorrhoeae MS11

42.515

100

0.452

  pilE Neisseria elongata subsp. glycolytica ATCC 29315

41.176

100

0.446

  pilA/pilA1 Eikenella corrodens VA1

41.975

100

0.433

  pilA2 Legionella pneumophila str. Paris

45.578

93.631

0.427

  pilA2 Legionella pneumophila strain ERS1305867

44.218

93.631

0.414

  pilA Ralstonia pseudosolanacearum GMI1000

39.394

100

0.414

  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.333

95.541

0.414

  pilA Pseudomonas aeruginosa PAK

41.722

96.178

0.401

  pilA Acinetobacter nosocomialis M2

55.357

71.338

0.395


Multiple sequence alignment