Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FK728_RS16525 Genome accession   NZ_CP041587
Coordinates   3480960..3481379 (-) Length   139 a.a.
NCBI ID   WP_000993722.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain J9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3475960..3486379
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FK728_RS16500 (FK728_03271) - 3476322..3476705 (+) 384 WP_000090000.1 RidA family protein -
  FK728_RS16505 (FK728_03272) - 3476915..3477109 (+) 195 WP_000289091.1 bacterioferritin-associated ferredoxin -
  FK728_RS16510 (FK728_03273) bfr 3477354..3477818 (+) 465 WP_000678123.1 heteropolymeric bacterioferritin subunit Bfr -
  FK728_RS16515 (FK728_03274) - 3477860..3479488 (-) 1629 WP_001195782.1 PglL family O-oligosaccharyltransferase -
  FK728_RS16520 (FK728_03275) - 3479596..3480906 (-) 1311 WP_000914626.1 O-antigen ligase family protein -
  FK728_RS16525 (FK728_03276) pilA 3480960..3481379 (-) 420 WP_000993722.1 pilin Machinery gene
  FK728_RS16530 (FK728_03277) - 3481722..3482282 (-) 561 WP_000258949.1 TPM domain-containing protein -
  FK728_RS16535 (FK728_03278) - 3482276..3483358 (-) 1083 WP_001246375.1 TPM domain-containing protein -
  FK728_RS16540 (FK728_03279) - 3483380..3483970 (-) 591 WP_000846931.1 LemA family protein -
  FK728_RS16545 (FK728_03280) - 3484101..3484988 (-) 888 WP_001017322.1 metal-dependent hydrolase -

Sequence

Protein


Download         Length: 139 a.a.        Molecular weight: 14367.31 Da        Isoelectric Point: 6.2180

>NTDB_id=327059 FK728_RS16525 WP_000993722.1 3480960..3481379(-) (pilA) [Acinetobacter baumannii strain J9]
MNAQKGFTLIELMIVVAIIGILAAIAIPAYSNYQARGKLTAGLSEISAGKAAAEQKINDGSDWTSPTDIGLQSTTSNCTI
TSTMSAGAGTIKCEVLNAPSQVRGAAITWTRDKAGTWSCATSGLDDDKKDLAPKSCPQS

Nucleotide


Download         Length: 420 bp        

>NTDB_id=327059 FK728_RS16525 WP_000993722.1 3480960..3481379(-) (pilA) [Acinetobacter baumannii strain J9]
ATGAATGCACAAAAAGGTTTTACATTAATTGAACTCATGATCGTGGTTGCCATTATCGGTATTTTGGCTGCAATTGCGAT
TCCTGCTTATAGTAATTATCAAGCACGAGGTAAATTAACAGCTGGCTTGTCAGAAATTTCTGCTGGAAAAGCGGCTGCTG
AACAAAAAATTAATGATGGTTCAGATTGGACATCACCGACAGATATTGGTCTACAAAGCACAACTTCTAATTGTACTATT
ACTTCGACAATGTCAGCAGGTGCAGGTACTATTAAATGTGAAGTATTAAATGCGCCTTCTCAAGTCCGTGGAGCAGCTAT
TACTTGGACAAGAGATAAAGCTGGTACTTGGTCTTGTGCTACATCTGGGCTAGATGATGATAAAAAAGATTTAGCTCCAA
AATCATGCCCACAATCTTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-136)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

44.681

100

0.453
  pilA Ralstonia pseudosolanacearum GMI1000

39.375

100

0.453
  pilA/pilAII Pseudomonas stutzeri DSM 10701

46.154

93.525

0.432
  comP Acinetobacter baylyi ADP1

39.735

100

0.432
  pilA Pseudomonas aeruginosa PAK

35.758

100

0.424
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.581

89.209

0.424
  pilA Vibrio cholerae C6706

37.5

100

0.388
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

37.5

100

0.388
  pilA Vibrio cholerae strain A1552

37.5

100

0.388
  pilA2 Legionella pneumophila str. Paris

44.628

87.05

0.388
  pilE Neisseria gonorrhoeae MS11

34.194

100

0.381
  pilA2 Legionella pneumophila strain ERS1305867

43.802

87.05

0.381

Multiple sequence alignment