Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   EFV54_RS11295 Genome accession   NZ_CP033607
Coordinates   2188173..2189111 (-) Length   312 a.a.
NCBI ID   WP_010906320.1    Uniprot ID   A0AAC9W673
Organism   Lactococcus lactis strain IL1403     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2183173..2194111
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EFV54_RS11255 (EFV54_11255) - 2184061..2184903 (-) 843 WP_010906312.1 metal ABC transporter substrate-binding protein -
  EFV54_RS11260 (EFV54_11260) - 2184900..2185337 (-) 438 WP_010906313.1 zinc-dependent MarR family transcriptional regulator -
  EFV54_RS11265 (EFV54_11265) comGG 2185418..2185702 (-) 285 WP_010906314.1 competence type IV pilus minor pilin ComGG Machinery gene
  EFV54_RS11270 (EFV54_11270) comGF 2185741..2186187 (-) 447 WP_164752712.1 competence type IV pilus minor pilin ComGF Machinery gene
  EFV54_RS11275 (EFV54_11275) comGE 2186150..2186446 (-) 297 WP_010906316.1 competence type IV pilus minor pilin ComGE Machinery gene
  EFV54_RS11280 (EFV54_11280) comGD 2186418..2186816 (-) 399 WP_021214886.1 competence type IV pilus minor pilin ComGD Machinery gene
  EFV54_RS11285 (EFV54_11285) comGC 2186809..2187192 (-) 384 WP_010906318.1 competence type IV pilus major pilin ComGC Machinery gene
  EFV54_RS11290 (EFV54_11290) comGB 2187206..2188225 (-) 1020 WP_043994702.1 competence type IV pilus assembly protein ComGB Machinery gene
  EFV54_RS11295 (EFV54_11295) comGA 2188173..2189111 (-) 939 WP_010906320.1 competence type IV pilus ATPase ComGA Machinery gene

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35719.89 Da        Isoelectric Point: 6.1804

>NTDB_id=324393 EFV54_RS11295 WP_010906320.1 2188173..2189111(-) (comGA) [Lactococcus lactis strain IL1403]
MIPKMAKELIQRAIDRDASDIYLIASKEKYKLYFRQMTARDLVEEIGLEVGLALLTHFKFLAGMNTGERRRVQLGACWYE
IEESNAKRLRLSTVGDFEGNESLVIRLLHDQNKELDFWFDQELENYDCKRGLHLFAGPVGSGKTSLMFDLARRHFTDLQV
INIEEPVELIDSDFIQLQVNEVIGNSYDELIKLSLRHRPDLLIVGEIRDKLTAQAVLRASLTGYTVFSTVHASSVRGVIQ
RLSELGLSHWEIKNALSSVVYQRLIAGKGVLDIAKNEFESWSSENWNKKIDQLLTNGYLTPTEAKREKIKTD

Nucleotide


Download         Length: 939 bp        

>NTDB_id=324393 EFV54_RS11295 WP_010906320.1 2188173..2189111(-) (comGA) [Lactococcus lactis strain IL1403]
ATGATACCAAAAATGGCAAAAGAATTAATTCAAAGAGCAATTGATAGAGATGCATCAGATATTTATCTGATTGCTTCAAA
AGAAAAGTATAAACTTTATTTTCGACAAATGACAGCACGTGATCTGGTAGAAGAAATTGGGTTAGAAGTTGGCTTAGCAC
TACTTACTCACTTTAAGTTTCTAGCTGGGATGAATACAGGAGAACGCAGACGAGTTCAATTGGGAGCTTGTTGGTATGAA
ATAGAGGAAAGTAATGCAAAACGTTTGCGTCTTTCGACAGTGGGGGATTTCGAGGGTAATGAATCTTTAGTAATACGTCT
CTTGCATGATCAAAACAAAGAACTTGATTTTTGGTTTGATCAAGAGCTAGAGAATTATGATTGTAAAAGAGGATTGCATC
TCTTTGCCGGTCCAGTAGGCTCGGGAAAAACATCTTTAATGTTTGATTTAGCTCGTCGTCATTTTACAGATTTACAAGTT
ATCAATATAGAAGAACCCGTTGAGTTGATTGATTCCGATTTTATTCAGTTACAAGTCAACGAAGTCATTGGTAATAGTTA
TGATGAATTAATTAAATTATCACTGAGGCATCGCCCAGATTTGTTAATCGTTGGTGAAATTCGTGATAAATTGACCGCTC
AAGCAGTTTTACGGGCTAGTTTGACAGGTTATACAGTGTTTTCAACCGTTCATGCCTCTTCTGTTAGAGGTGTCATTCAG
AGATTATCAGAGCTGGGTTTAAGTCATTGGGAAATAAAAAATGCTTTATCAAGTGTTGTTTATCAGCGTTTAATAGCTGG
GAAAGGAGTATTAGACATTGCCAAAAACGAATTTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTAC
TTACAAATGGATATCTCACACCTACTGAAGCCAAAAGGGAAAAAATTAAGACTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

76.603

100

0.766
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

54.662

99.679

0.545
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.055

99.679

0.529
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

52.09

99.679

0.519
  comGA/cglA/cilD Streptococcus pneumoniae D39

52.09

99.679

0.519
  comGA/cglA/cilD Streptococcus pneumoniae R6

52.09

99.679

0.519
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

52.09

99.679

0.519
  comYA Streptococcus gordonii str. Challis substr. CH1

51.923

100

0.519
  comYA Streptococcus mutans UA140

51.923

100

0.519
  comYA Streptococcus mutans UA159

51.923

100

0.519

Multiple sequence alignment