Detailed information NTG0324388

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: EFV54_RS11270
• Genome accession: NZ_CP033607
• Coordinates: 2185741..2186187 (-)
• Length: 148 a.a.
• NCBI ID: WP_164752712.1
• Uniprot ID: -
• Organism: Lactococcus lactis strain IL1403
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2180741..2191187

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EFV54_RS11235 (EFV54_11235)	-	2180858..2181235 (-)	378	WP_003129983.1	pyridoxamine 5'-phosphate oxidase family protein	-
EFV54_RS11240 (EFV54_11240)	-	2181440..2182306 (+)	867	WP_010906309.1	RluA family pseudouridine synthase	-
EFV54_RS11245 (EFV54_11245)	-	2182345..2183154 (-)	810	WP_010906310.1	metal ABC transporter permease	-
EFV54_RS11250 (EFV54_11250)	-	2183147..2183884 (-)	738	WP_010906311.1	metal ABC transporter ATP-binding protein	-
EFV54_RS11255 (EFV54_11255)	-	2184061..2184903 (-)	843	WP_010906312.1	metal ABC transporter substrate-binding protein	-
EFV54_RS11260 (EFV54_11260)	-	2184900..2185337 (-)	438	WP_010906313.1	zinc-dependent MarR family transcriptional regulator	-
EFV54_RS11265 (EFV54_11265)	comGG	2185418..2185702 (-)	285	WP_010906314.1	competence type IV pilus minor pilin ComGG	Machinery gene
EFV54_RS11270 (EFV54_11270)	comGF	2185741..2186187 (-)	447	WP_164752712.1	competence type IV pilus minor pilin ComGF	Machinery gene
EFV54_RS11275 (EFV54_11275)	comGE	2186150..2186446 (-)	297	WP_010906316.1	competence type IV pilus minor pilin ComGE	Machinery gene
EFV54_RS11280 (EFV54_11280)	comGD	2186418..2186816 (-)	399	WP_021214886.1	competence type IV pilus minor pilin ComGD	Machinery gene
EFV54_RS11285 (EFV54_11285)	comGC	2186809..2187192 (-)	384	WP_010906318.1	competence type IV pilus major pilin ComGC	Machinery gene
EFV54_RS11290 (EFV54_11290)	comGB	2187206..2188225 (-)	1020	WP_043994702.1	competence type IV pilus assembly protein ComGB	Machinery gene
EFV54_RS11295 (EFV54_11295)	comGA	2188173..2189111 (-)	939	WP_010906320.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 17083.85 Da
• Isoelectric Point: 8.6780

>NTDB_id=324388 EFV54_RS11270 WP_164752712.1 2185741..2186187(-) (comGF) [Lactococcus lactis strain IL1403]
MERKLCDLNFKVKAFTLLECLVALLAISGSALVISGLTKMLKEQVALSQSDSIKDWQIFCQQMRFELSGTKLDRVEQNFL
YVTKDKKLRFGFMSDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide

• Length: 447 bp

>NTDB_id=324388 EFV54_RS11270 WP_164752712.1 2185741..2186187(-) (comGF) [Lactococcus lactis strain IL1403]
ATGGAAAGGAAATTATGCGACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGCCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCGCTTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAACAGAATTTTCTG
TATGTAACTAAAGATAAAAAACTAAGGTTTGGTTTTATGAGTGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAAAGAACATTTATTTATCAATTTCCAGAAGATACGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (46-137)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	74.82	93.919	0.703
comYF	Streptococcus mutans UA140	47.518	95.27	0.453
comYF	Streptococcus mutans UA159	46.809	95.27	0.446
comGF/cglF	Streptococcus mitis SK321	43.066	92.568	0.399
comGF/cglF	Streptococcus pneumoniae Rx1	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae D39	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae R6	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae TIGR4	40.426	95.27	0.385
comGF/cglF	Streptococcus mitis NCTC 12261	41.606	92.568	0.385