Detailed information    

experimental Experimentally validated

Overview

Name   letA   Type   Regulator
Locus tag   GCO85_RS13310 Genome accession   NZ_CP048618
Coordinates   2996854..2997513 (-) Length   219 a.a.
NCBI ID   WP_011947563.1    Uniprot ID   -
Organism   Legionella pneumophila strain ERS1305867     
Function   regulate competence development   
Competence regulation

Function

Mutation of this gene caused a decrease in transformation. LetA/S TCS is involved in the regulation of competence upstream of the regulation controlled by the RocC/RocR system.


Genomic Context

Location: 2991854..3002513
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GCO85_RS13295 (GCO85_13305) - 2992726..2993385 (+) 660 WP_011947560.1 hypothetical protein -
  GCO85_RS13300 (GCO85_13310) - 2993553..2994962 (+) 1410 WP_061637081.1 DUF1566 domain-containing protein -
  GCO85_RS13305 (GCO85_13315) uvrC 2994986..2996842 (-) 1857 WP_042754708.1 excinuclease ABC subunit UvrC -
  GCO85_RS13310 (GCO85_13320) letA 2996854..2997513 (-) 660 WP_011947563.1 UvrY/SirA/GacA family response regulator transcription factor Regulator
  GCO85_RS13315 (GCO85_13325) phhA 2997638..2998456 (-) 819 WP_015961727.1 phenylalanine 4-monooxygenase -
  GCO85_RS13320 (GCO85_13330) - 2998896..2999993 (+) 1098 WP_027226738.1 competence/damage-inducible protein A -
  GCO85_RS13325 (GCO85_13335) cgtA 2999952..3000977 (-) 1026 WP_015961729.1 Obg family GTPase CgtA -
  GCO85_RS13330 (GCO85_13340) rpmA 3001191..3001469 (-) 279 WP_010948350.1 50S ribosomal protein L27 -
  GCO85_RS13335 (GCO85_13345) rplU 3001482..3001793 (-) 312 WP_010948351.1 50S ribosomal protein L21 -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24566.53 Da        Isoelectric Point: 5.1572

>NTDB_id=1262 GCO85_RS13310 WP_011947563.1 2996854..2997513(-) (letA) [Legionella pneumophila strain ERS1305867]
MIKVLIVDDHALVRMGIRRLLEDMSDVDVVADAESGEQALAYVKTHSPDVVLLDMKMPGIDGWEVTRRLKKTNPNIKVIA
VTAICSDPLPTRVLQLGAMGYLTKESGAEEMAAAIRKVAKGEKYLSAEIAQKMAINSLQEPQDSPFDLLSEREMQVMLMI
TSGMNVQDIADRLFLSSKTINGYRYRMFEKLGIKNDVELTYLAMKHRIIEHPNDLSQDD

Nucleotide


Download         Length: 660 bp        

>NTDB_id=1262 GCO85_RS13310 WP_011947563.1 2996854..2997513(-) (letA) [Legionella pneumophila strain ERS1305867]
TTGATTAAAGTATTAATTGTTGATGACCATGCATTGGTTAGAATGGGCATTCGACGATTGCTTGAAGATATGTCGGATGT
TGATGTGGTTGCAGATGCTGAAAGTGGTGAGCAGGCTTTAGCTTATGTGAAAACCCACTCCCCTGATGTCGTATTATTAG
ACATGAAAATGCCTGGGATAGATGGGTGGGAAGTGACACGTCGTTTAAAAAAAACAAACCCAAATATTAAAGTCATAGCC
GTTACAGCTATTTGCTCAGATCCTCTCCCTACTCGAGTATTGCAATTAGGTGCTATGGGATACCTTACTAAAGAATCAGG
GGCTGAAGAAATGGCTGCTGCAATTCGTAAAGTGGCTAAAGGTGAGAAATATCTTAGTGCCGAGATTGCACAAAAAATGG
CAATTAATAGTCTTCAGGAGCCTCAGGATTCCCCCTTTGATTTATTGTCTGAAAGAGAAATGCAAGTCATGTTGATGATT
ACGAGCGGCATGAATGTACAAGACATTGCTGATAGATTATTTTTAAGTAGTAAAACCATCAATGGTTATCGATATAGGAT
GTTTGAAAAATTGGGGATTAAAAATGATGTGGAGCTTACTTATTTGGCTATGAAGCACCGCATTATTGAACACCCCAATG
ATTTGTCACAAGATGATTAG

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(4-116)

Bacterial regulatory proteins, luxR family (GerE)

(148-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  letA Legionella pneumophila str. Paris

100

100

1

Multiple sequence alignment    



References

[1] Léo Hardy et al. (2021) Transposon Insertion Sequencing in a Clinical Isolate of Legionella pneumophila Identifies Essential Genes and Determinants of Natural Transformation. Journal of Bacteriology 203(3):e00548-20. [PMID: 33168636]