Detailed information    

experimental Experimentally validated

Overview

Name   letA   Type   Regulator
Locus tag   LPP_RS13600 Genome accession   NC_006368
Coordinates   3080823..3081482 (-) Length   219 a.a.
NCBI ID   WP_011947563.1    Uniprot ID   -
Organism   Legionella pneumophila str. Paris     
Function   regulate competence development   
Competence regulation

Function

Mutation of this gene caused a decrease in transformation.


Genomic Context

Location: 3075823..3086482
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPP_RS13585 (lpp2696) - 3076956..3077615 (+) 660 WP_015961724.1 hypothetical protein -
  LPP_RS13590 (lpp2697) lcl 3077783..3078931 (+) 1149 WP_015961725.1 collagen-like adhesin Lcl -
  LPP_RS13595 (lpp2698) uvrC 3078955..3080811 (-) 1857 WP_015961726.1 excinuclease ABC subunit UvrC -
  LPP_RS13600 (lpp2699) letA 3080823..3081482 (-) 660 WP_011947563.1 two-component system response regulator LetA Regulator
  LPP_RS13605 (lpp2700) phhA 3081607..3082425 (-) 819 WP_015961727.1 phenylalanine 4-monooxygenase -
  LPP_RS13610 (lpp2701) - 3082865..3083962 (+) 1098 WP_015961728.1 competence/damage-inducible protein A -
  LPP_RS13615 (lpp2702) cgtA 3083921..3084946 (-) 1026 WP_015961729.1 Obg family GTPase CgtA -
  LPP_RS13620 (lpp2703) rpmA 3085160..3085438 (-) 279 WP_010948350.1 50S ribosomal protein L27 -
  LPP_RS13625 (lpp2704) rplU 3085451..3085762 (-) 312 WP_010948351.1 50S ribosomal protein L21 -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24566.53 Da        Isoelectric Point: 5.1572

>NTDB_id=1278 LPP_RS13600 WP_011947563.1 3080823..3081482(-) (letA) [Legionella pneumophila str. Paris]
MIKVLIVDDHALVRMGIRRLLEDMSDVDVVADAESGEQALAYVKTHSPDVVLLDMKMPGIDGWEVTRRLKKTNPNIKVIA
VTAICSDPLPTRVLQLGAMGYLTKESGAEEMAAAIRKVAKGEKYLSAEIAQKMAINSLQEPQDSPFDLLSEREMQVMLMI
TSGMNVQDIADRLFLSSKTINGYRYRMFEKLGIKNDVELTYLAMKHRIIEHPNDLSQDD

Nucleotide


Download         Length: 660 bp        

>NTDB_id=1278 LPP_RS13600 WP_011947563.1 3080823..3081482(-) (letA) [Legionella pneumophila str. Paris]
TTGATTAAAGTATTAATTGTTGATGACCATGCATTGGTTAGAATGGGCATTCGACGATTGCTTGAAGATATGTCGGATGT
TGATGTGGTTGCAGATGCTGAAAGTGGTGAGCAGGCTTTAGCTTATGTGAAAACCCACTCCCCTGATGTCGTATTATTAG
ACATGAAAATGCCTGGGATAGATGGGTGGGAAGTGACACGTCGTTTAAAAAAAACAAACCCAAATATTAAAGTCATAGCC
GTTACAGCTATTTGCTCAGATCCTCTCCCTACTCGAGTATTGCAATTAGGTGCTATGGGATACCTTACTAAAGAATCAGG
GGCTGAAGAAATGGCTGCTGCAATTCGTAAAGTGGCTAAAGGTGAGAAATATCTTAGTGCCGAGATTGCACAAAAAATGG
CAATTAATAGTCTTCAGGAGCCTCAGGATTCCCCCTTTGATTTATTGTCTGAAAGAGAAATGCAAGTCATGTTGATGATT
ACGAGCGGCATGAATGTACAAGACATTGCTGATAGATTATTTTTAAGTAGTAAAACCATCAATGGTTATCGATATAGGAT
GTTTGAAAAATTGGGGATTAAAAATGATGTGGAGCTTACTTATTTGGCTATGAAGCACCGCATTATTGAACACCCCAATG
ATTTGTCACAAGATGATTAG

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(4-116)

Bacterial regulatory proteins, luxR family (GerE)

(148-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  letA Legionella pneumophila strain ERS1305867

100

100

1

Multiple sequence alignment    



References

[1] Léo Hardy et al. (2021) Transposon Insertion Sequencing in a Clinical Isolate of Legionella pneumophila Identifies Essential Genes and Determinants of Natural Transformation. Journal of Bacteriology 203(3):e00548-20. [PMID: 33168636]