Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   EFV54_RS11290 Genome accession   NZ_CP033607
Coordinates   2187206..2188225 (-) Length   339 a.a.
NCBI ID   WP_043994702.1    Uniprot ID   -
Organism   Lactococcus lactis strain IL1403     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2182206..2193225
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EFV54_RS11245 (EFV54_11245) - 2182345..2183154 (-) 810 WP_010906310.1 metal ABC transporter permease -
  EFV54_RS11250 (EFV54_11250) - 2183147..2183884 (-) 738 WP_010906311.1 metal ABC transporter ATP-binding protein -
  EFV54_RS11255 (EFV54_11255) - 2184061..2184903 (-) 843 WP_010906312.1 metal ABC transporter substrate-binding protein -
  EFV54_RS11260 (EFV54_11260) - 2184900..2185337 (-) 438 WP_010906313.1 zinc-dependent MarR family transcriptional regulator -
  EFV54_RS11265 (EFV54_11265) comGG 2185418..2185702 (-) 285 WP_010906314.1 competence type IV pilus minor pilin ComGG Machinery gene
  EFV54_RS11270 (EFV54_11270) comGF 2185741..2186187 (-) 447 WP_164752712.1 competence type IV pilus minor pilin ComGF Machinery gene
  EFV54_RS11275 (EFV54_11275) comGE 2186150..2186446 (-) 297 WP_010906316.1 competence type IV pilus minor pilin ComGE Machinery gene
  EFV54_RS11280 (EFV54_11280) comGD 2186418..2186816 (-) 399 WP_021214886.1 competence type IV pilus minor pilin ComGD Machinery gene
  EFV54_RS11285 (EFV54_11285) comGC 2186809..2187192 (-) 384 WP_010906318.1 competence type IV pilus major pilin ComGC Machinery gene
  EFV54_RS11290 (EFV54_11290) comGB 2187206..2188225 (-) 1020 WP_043994702.1 competence type IV pilus assembly protein ComGB Machinery gene
  EFV54_RS11295 (EFV54_11295) comGA 2188173..2189111 (-) 939 WP_010906320.1 competence type IV pilus ATPase ComGA Machinery gene

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 38291.00 Da        Isoelectric Point: 10.1935

>NTDB_id=324392 EFV54_RS11290 WP_043994702.1 2187206..2188225(-) (comGB) [Lactococcus lactis strain IL1403]
MDISHLLKPKGKKLRLIKQAKLIQLMGNLLNSGFNLGEVINFLSLSKLVEKEFTLKMKEGLASGQALSELLESLSFSKNV
VTQLALVEVHGNLSGTMQLVELHLKKQLKVKNKLVEVATYPILLLIFLVGIMWGLKNYLLPQLNSGSNFATLLINHLPLV
FFSFGAFIFLLTALSVTLFKRKSAIMNFTFLVKLPLVHSFIRLYLTAYFAREWGNLIAQGVELRQIINLMKKQKSRIFSE
VGKNLDLELNAGRSFEQAVSKLALFLPELSLMIEYGAIKDKLGLELSLYADECWEQFFTKIDRLMQLIQPLVFIFVALMI
ILLYAAMLLPIYSNMGSGI

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=324392 EFV54_RS11290 WP_043994702.1 2187206..2188225(-) (comGB) [Lactococcus lactis strain IL1403]
ATGGATATCTCACACCTACTGAAGCCAAAAGGGAAAAAATTAAGACTGATTAAACAAGCCAAACTTATCCAACTGATGGG
AAATCTTTTGAATAGTGGATTTAATTTAGGAGAAGTGATAAATTTCTTATCTTTATCAAAACTTGTGGAAAAAGAATTTA
CATTAAAAATGAAAGAAGGTCTCGCTTCTGGTCAAGCTTTATCAGAGCTTTTAGAAAGTCTTTCATTTTCTAAAAATGTG
GTGACACAACTTGCTTTGGTAGAAGTGCATGGTAACTTGTCAGGGACGATGCAATTAGTTGAACTTCATCTAAAAAAACA
GCTTAAAGTTAAAAATAAATTAGTGGAAGTTGCTACTTATCCAATATTGTTATTAATTTTTCTGGTTGGAATTATGTGGG
GCTTAAAAAATTATTTACTACCACAACTCAATAGTGGCAGTAACTTTGCTACTCTATTGATTAATCATTTACCCTTAGTA
TTTTTTTCGTTTGGAGCTTTTATATTTTTACTGACAGCTTTGTCAGTAACTCTTTTTAAACGAAAATCAGCAATAATGAA
TTTTACATTTCTTGTTAAATTACCTCTGGTTCATTCTTTTATCCGTTTATATTTAACAGCTTACTTTGCAAGAGAATGGG
GAAATCTAATTGCTCAAGGTGTTGAATTACGTCAAATTATTAACTTGATGAAAAAACAAAAAAGTCGAATTTTTTCAGAA
GTTGGCAAGAATCTTGATTTAGAATTGAATGCTGGTCGGTCTTTTGAACAAGCTGTCAGTAAACTTGCGCTTTTTTTACC
CGAGCTCTCGTTAATGATTGAATATGGAGCTATTAAAGATAAATTAGGCTTAGAGCTTTCGCTTTATGCGGATGAATGTT
GGGAACAATTTTTTACAAAGATAGATCGTTTAATGCAATTAATCCAACCTCTTGTTTTTATTTTCGTAGCATTGATGATT
ATTTTGCTATATGCAGCCATGTTACTCCCCATCTATTCAAATATGGGTTCGGGAATATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(23-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-330)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

84.024

99.705

0.838
  comYB Streptococcus gordonii str. Challis substr. CH1

53.892

98.525

0.531
  comGB/cglB Streptococcus mitis SK321

51.343

98.82

0.507
  comGB/cglB Streptococcus pneumoniae TIGR4

51.045

98.82

0.504
  comGB/cglB Streptococcus pneumoniae Rx1

51.045

98.82

0.504
  comGB/cglB Streptococcus pneumoniae D39

51.045

98.82

0.504
  comGB/cglB Streptococcus pneumoniae R6

51.045

98.82

0.504
  comGB/cglB Streptococcus mitis NCTC 12261

50.448

98.82

0.499
  comYB Streptococcus mutans UA140

53.651

92.92

0.499
  comYB Streptococcus mutans UA159

53.333

92.92

0.496

Multiple sequence alignment