Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   EFD52_RS10005 Genome accession   NZ_CP033606
Coordinates   2030719..2031657 (-) Length   312 a.a.
NCBI ID   WP_010906320.1    Uniprot ID   A0AAC9W673
Organism   Lactococcus lactis strain IL6288     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2025719..2036657
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EFD52_RS09965 (EFD52_09965) - 2026607..2027449 (-) 843 WP_010906312.1 metal ABC transporter substrate-binding protein -
  EFD52_RS09970 (EFD52_09970) - 2027446..2027883 (-) 438 WP_010906313.1 zinc-dependent MarR family transcriptional regulator -
  EFD52_RS09975 (EFD52_09975) comGG 2027964..2028248 (-) 285 WP_010906314.1 competence type IV pilus minor pilin ComGG Machinery gene
  EFD52_RS09980 (EFD52_09980) comGF 2028287..2028733 (-) 447 WP_164752712.1 competence type IV pilus minor pilin ComGF Machinery gene
  EFD52_RS09985 (EFD52_09985) comGE 2028696..2028992 (-) 297 WP_010906316.1 competence type IV pilus minor pilin ComGE Machinery gene
  EFD52_RS09990 (EFD52_09990) comGD 2028964..2029362 (-) 399 WP_021214886.1 competence type IV pilus minor pilin ComGD Machinery gene
  EFD52_RS09995 (EFD52_09995) comGC 2029355..2029738 (-) 384 WP_010906318.1 competence type IV pilus major pilin ComGC Machinery gene
  EFD52_RS10000 (EFD52_10000) comGB 2029752..2030825 (-) 1074 WP_010906319.1 competence type IV pilus assembly protein ComGB Machinery gene
  EFD52_RS10005 (EFD52_10005) comGA 2030719..2031657 (-) 939 WP_010906320.1 competence type IV pilus ATPase ComGA Machinery gene

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35719.89 Da        Isoelectric Point: 6.1804

>NTDB_id=324343 EFD52_RS10005 WP_010906320.1 2030719..2031657(-) (comGA) [Lactococcus lactis strain IL6288]
MIPKMAKELIQRAIDRDASDIYLIASKEKYKLYFRQMTARDLVEEIGLEVGLALLTHFKFLAGMNTGERRRVQLGACWYE
IEESNAKRLRLSTVGDFEGNESLVIRLLHDQNKELDFWFDQELENYDCKRGLHLFAGPVGSGKTSLMFDLARRHFTDLQV
INIEEPVELIDSDFIQLQVNEVIGNSYDELIKLSLRHRPDLLIVGEIRDKLTAQAVLRASLTGYTVFSTVHASSVRGVIQ
RLSELGLSHWEIKNALSSVVYQRLIAGKGVLDIAKNEFESWSSENWNKKIDQLLTNGYLTPTEAKREKIKTD

Nucleotide


Download         Length: 939 bp        

>NTDB_id=324343 EFD52_RS10005 WP_010906320.1 2030719..2031657(-) (comGA) [Lactococcus lactis strain IL6288]
ATGATACCAAAAATGGCAAAAGAATTAATTCAAAGAGCAATTGATAGAGATGCATCAGATATTTATCTGATTGCTTCAAA
AGAAAAGTATAAACTTTATTTTCGACAAATGACAGCACGTGATCTGGTAGAAGAAATTGGGTTAGAAGTTGGCTTAGCAC
TACTTACTCACTTTAAGTTTCTAGCTGGGATGAATACAGGAGAACGCAGACGAGTTCAATTGGGAGCTTGTTGGTATGAA
ATAGAGGAAAGTAATGCAAAACGTTTGCGTCTTTCGACAGTGGGGGATTTCGAGGGTAATGAATCTTTAGTAATACGTCT
CTTGCATGATCAAAACAAAGAACTTGATTTTTGGTTTGATCAAGAGCTAGAGAATTATGATTGTAAAAGAGGATTGCATC
TCTTTGCCGGTCCAGTAGGCTCGGGAAAAACATCTTTAATGTTTGATTTAGCTCGTCGTCATTTTACAGATTTACAAGTT
ATCAATATAGAAGAACCCGTTGAGTTGATTGATTCCGATTTTATTCAGTTACAAGTCAACGAAGTCATTGGTAATAGTTA
TGATGAATTAATTAAATTATCACTGAGGCATCGCCCAGATTTGTTAATCGTTGGTGAAATTCGTGATAAATTGACCGCTC
AAGCAGTTTTACGGGCTAGTTTGACAGGTTATACAGTGTTTTCAACCGTTCATGCCTCTTCTGTTAGAGGTGTCATTCAG
AGATTATCAGAGCTGGGTTTAAGTCATTGGGAAATAAAAAATGCTTTATCAAGTGTTGTTTATCAGCGTTTAATAGCTGG
GAAAGGAGTATTAGACATTGCCAAAAACGAATTTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTAC
TTACAAATGGATATCTCACACCTACTGAAGCCAAAAGGGAAAAAATTAAGACTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

76.603

100

0.766
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

54.662

99.679

0.545
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.055

99.679

0.529
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

52.09

99.679

0.519
  comGA/cglA/cilD Streptococcus pneumoniae D39

52.09

99.679

0.519
  comGA/cglA/cilD Streptococcus pneumoniae R6

52.09

99.679

0.519
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

52.09

99.679

0.519
  comYA Streptococcus gordonii str. Challis substr. CH1

51.923

100

0.519
  comYA Streptococcus mutans UA140

51.923

100

0.519
  comYA Streptococcus mutans UA159

51.923

100

0.519

Multiple sequence alignment