Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGD   Type   Machinery gene
Locus tag   EFD52_RS09990 Genome accession   NZ_CP033606
Coordinates   2028964..2029362 (-) Length   132 a.a.
NCBI ID   WP_021214886.1    Uniprot ID   -
Organism   Lactococcus lactis strain IL6288     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2023964..2034362
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EFD52_RS09950 (EFD52_09950) - 2023986..2024852 (+) 867 WP_010906309.1 RluA family pseudouridine synthase -
  EFD52_RS09955 (EFD52_09955) - 2024891..2025700 (-) 810 WP_010906310.1 metal ABC transporter permease -
  EFD52_RS09960 (EFD52_09960) - 2025693..2026430 (-) 738 WP_010906311.1 metal ABC transporter ATP-binding protein -
  EFD52_RS09965 (EFD52_09965) - 2026607..2027449 (-) 843 WP_010906312.1 metal ABC transporter substrate-binding protein -
  EFD52_RS09970 (EFD52_09970) - 2027446..2027883 (-) 438 WP_010906313.1 zinc-dependent MarR family transcriptional regulator -
  EFD52_RS09975 (EFD52_09975) comGG 2027964..2028248 (-) 285 WP_010906314.1 competence type IV pilus minor pilin ComGG Machinery gene
  EFD52_RS09980 (EFD52_09980) comGF 2028287..2028733 (-) 447 WP_164752712.1 competence type IV pilus minor pilin ComGF Machinery gene
  EFD52_RS09985 (EFD52_09985) comGE 2028696..2028992 (-) 297 WP_010906316.1 competence type IV pilus minor pilin ComGE Machinery gene
  EFD52_RS09990 (EFD52_09990) comGD 2028964..2029362 (-) 399 WP_021214886.1 competence type IV pilus minor pilin ComGD Machinery gene
  EFD52_RS09995 (EFD52_09995) comGC 2029355..2029738 (-) 384 WP_010906318.1 competence type IV pilus major pilin ComGC Machinery gene
  EFD52_RS10000 (EFD52_10000) comGB 2029752..2030825 (-) 1074 WP_010906319.1 competence type IV pilus assembly protein ComGB Machinery gene
  EFD52_RS10005 (EFD52_10005) comGA 2030719..2031657 (-) 939 WP_010906320.1 competence type IV pilus ATPase ComGA Machinery gene

Sequence

Protein


Download         Length: 132 a.a.        Molecular weight: 15173.79 Da        Isoelectric Point: 7.9839

>NTDB_id=324340 EFD52_RS09990 WP_021214886.1 2028964..2029362(-) (comGD) [Lactococcus lactis strain IL6288]
MTKAFTLLESLLVLLIISFITTLFSLEIIQTIHLFKGELFVLQFENFYKRSQEDAALLQKSESLVAKNQELICEDRSITI
PKEVAVKDFTVKFDDKGENSSLQKLTISLPYEKKFITYQLEIGSGKFKKKIS

Nucleotide


Download         Length: 399 bp        

>NTDB_id=324340 EFD52_RS09990 WP_021214886.1 2028964..2029362(-) (comGD) [Lactococcus lactis strain IL6288]
ATGACTAAAGCATTTACTTTACTAGAGTCTCTTCTAGTTTTGTTGATTATTTCTTTTATCACAACTCTTTTTTCTTTAGA
AATAATACAAACAATCCATCTTTTTAAGGGAGAATTGTTTGTTCTTCAATTTGAAAATTTCTATAAAAGGAGTCAAGAAG
ATGCTGCACTGCTTCAAAAATCTGAAAGTTTAGTTGCTAAAAATCAAGAATTAATCTGTGAAGATAGAAGTATCACAATT
CCAAAGGAGGTAGCAGTTAAAGATTTTACAGTTAAATTTGATGATAAGGGAGAGAATTCTAGCTTACAAAAACTCACAAT
TTCTTTACCTTACGAAAAAAAGTTCATCACTTATCAATTGGAGATAGGCAGTGGAAAATTTAAAAAGAAAATCAGTTAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGD Lactococcus lactis subsp. cremoris KW2

67.424

100

0.674
  comYD Streptococcus mutans UA140

40.625

96.97

0.394
  comYD Streptococcus mutans UA159

40.625

96.97

0.394
  comYD Streptococcus gordonii str. Challis substr. CH1

40.625

96.97

0.394

Multiple sequence alignment