Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   EFD52_RS10000 Genome accession   NZ_CP033606
Coordinates   2029752..2030825 (-) Length   357 a.a.
NCBI ID   WP_010906319.1    Uniprot ID   A0A552YT77
Organism   Lactococcus lactis strain IL6288     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2024752..2035825
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EFD52_RS09955 (EFD52_09955) - 2024891..2025700 (-) 810 WP_010906310.1 metal ABC transporter permease -
  EFD52_RS09960 (EFD52_09960) - 2025693..2026430 (-) 738 WP_010906311.1 metal ABC transporter ATP-binding protein -
  EFD52_RS09965 (EFD52_09965) - 2026607..2027449 (-) 843 WP_010906312.1 metal ABC transporter substrate-binding protein -
  EFD52_RS09970 (EFD52_09970) - 2027446..2027883 (-) 438 WP_010906313.1 zinc-dependent MarR family transcriptional regulator -
  EFD52_RS09975 (EFD52_09975) comGG 2027964..2028248 (-) 285 WP_010906314.1 competence type IV pilus minor pilin ComGG Machinery gene
  EFD52_RS09980 (EFD52_09980) comGF 2028287..2028733 (-) 447 WP_164752712.1 competence type IV pilus minor pilin ComGF Machinery gene
  EFD52_RS09985 (EFD52_09985) comGE 2028696..2028992 (-) 297 WP_010906316.1 competence type IV pilus minor pilin ComGE Machinery gene
  EFD52_RS09990 (EFD52_09990) comGD 2028964..2029362 (-) 399 WP_021214886.1 competence type IV pilus minor pilin ComGD Machinery gene
  EFD52_RS09995 (EFD52_09995) comGC 2029355..2029738 (-) 384 WP_010906318.1 competence type IV pilus major pilin ComGC Machinery gene
  EFD52_RS10000 (EFD52_10000) comGB 2029752..2030825 (-) 1074 WP_010906319.1 competence type IV pilus assembly protein ComGB Machinery gene
  EFD52_RS10005 (EFD52_10005) comGA 2030719..2031657 (-) 939 WP_010906320.1 competence type IV pilus ATPase ComGA Machinery gene

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 40330.51 Da        Isoelectric Point: 10.3000

>NTDB_id=324342 EFD52_RS10000 WP_010906319.1 2029752..2030825(-) (comGB) [Lactococcus lactis strain IL6288]
MSLGHQKIGIKKLISYLQMDISHLLKPKGKKLRLIKQAKLIQLMGNLLNSGFNLGEVINFLSLSKLVEKEFTLKMKEGLA
SGQALSELLESLSFSKNVVTQLALVEVHGNLSGTMQLVELHLKKQLKVKNKLVEVATYPILLLIFLVGIMWGLKNYLLPQ
LNSGSNFATLLINHLPLVFFSFGAFIFLLTALSVTLFKRKSAIMNFTFLVKLPLVHSFIRLYLTAYFAREWGNLIAQGVE
LRQIINLMKKQKSRIFSEVGKNLDLELNAGRSFEQAVSKLALFLPELSLMIEYGAIKDKLGLELSLYADECWEQFFTKID
RLMQLIQPLVFIFVALMIILLYAAMLLPIYSNMGSGI

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=324342 EFD52_RS10000 WP_010906319.1 2029752..2030825(-) (comGB) [Lactococcus lactis strain IL6288]
TTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTACTTACAAATGGATATCTCACACCTACTGAAGCC
AAAAGGGAAAAAATTAAGACTGATTAAACAAGCCAAACTTATCCAACTGATGGGAAATCTTTTGAATAGTGGATTTAATT
TAGGAGAAGTGATAAATTTCTTATCTTTATCAAAACTTGTGGAAAAAGAATTTACATTAAAAATGAAAGAAGGTCTCGCT
TCTGGTCAAGCTTTATCAGAGCTTTTAGAAAGTCTTTCATTTTCTAAAAATGTGGTGACACAACTTGCTTTGGTAGAAGT
GCATGGTAACTTGTCAGGGACGATGCAATTAGTTGAACTTCATCTAAAAAAACAGCTTAAAGTTAAAAATAAATTAGTGG
AAGTTGCTACTTATCCAATATTGTTATTAATTTTTCTGGTTGGAATTATGTGGGGCTTAAAAAATTATTTACTACCACAA
CTCAATAGTGGCAGTAACTTTGCTACTCTATTGATTAATCATTTACCCTTAGTATTTTTTTCGTTTGGAGCTTTTATATT
TTTACTGACAGCTTTGTCAGTAACTCTTTTTAAACGAAAATCAGCAATAATGAATTTTACATTTCTTGTTAAATTACCTC
TGGTTCATTCTTTTATCCGTTTATATTTAACAGCTTACTTTGCAAGAGAATGGGGAAATCTAATTGCTCAAGGTGTTGAA
TTACGTCAAATTATTAACTTGATGAAAAAACAAAAAAGTCGAATTTTTTCAGAAGTTGGCAAGAATCTTGATTTAGAATT
GAATGCTGGTCGGTCTTTTGAACAAGCTGTCAGTAAACTTGCGCTTTTTTTACCCGAGCTCTCGTTAATGATTGAATATG
GAGCTATTAAAGATAAATTAGGCTTAGAGCTTTCGCTTTATGCGGATGAATGTTGGGAACAATTTTTTACAAAGATAGAT
CGTTTAATGCAATTAATCCAACCTCTTGTTTTTATTTTCGTAGCATTGATGATTATTTTGCTATATGCAGCCATGTTACT
CCCCATCTATTCAAATATGGGTTCGGGAATATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(227-348)

Type II secretion system (T2SS), protein F (T2SSF)

(41-159)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A552YT77

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

83.578

95.518

0.798
  comYB Streptococcus gordonii str. Challis substr. CH1

53.959

95.518

0.515
  comYB Streptococcus mutans UA140

53.372

95.518

0.51
  comYB Streptococcus mutans UA159

53.079

95.518

0.507
  comGB/cglB Streptococcus mitis SK321

51.343

93.838

0.482
  comGB/cglB Streptococcus pneumoniae TIGR4

51.045

93.838

0.479
  comGB/cglB Streptococcus pneumoniae Rx1

51.045

93.838

0.479
  comGB/cglB Streptococcus pneumoniae D39

51.045

93.838

0.479
  comGB/cglB Streptococcus pneumoniae R6

51.045

93.838

0.479
  comGB/cglB Streptococcus mitis NCTC 12261

50.448

93.838

0.473

Multiple sequence alignment