Detailed information NTG0324338

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: EFD52_RS09980
• Genome accession: NZ_CP033606
• Coordinates: 2028287..2028733 (-)
• Length: 148 a.a.
• NCBI ID: WP_164752712.1
• Uniprot ID: -
• Organism: Lactococcus lactis strain IL6288
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2023287..2033733

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EFD52_RS09945 (EFD52_09945)	-	2023404..2023781 (-)	378	WP_003129983.1	pyridoxamine 5'-phosphate oxidase family protein	-
EFD52_RS09950 (EFD52_09950)	-	2023986..2024852 (+)	867	WP_010906309.1	RluA family pseudouridine synthase	-
EFD52_RS09955 (EFD52_09955)	-	2024891..2025700 (-)	810	WP_010906310.1	metal ABC transporter permease	-
EFD52_RS09960 (EFD52_09960)	-	2025693..2026430 (-)	738	WP_010906311.1	metal ABC transporter ATP-binding protein	-
EFD52_RS09965 (EFD52_09965)	-	2026607..2027449 (-)	843	WP_010906312.1	metal ABC transporter substrate-binding protein	-
EFD52_RS09970 (EFD52_09970)	-	2027446..2027883 (-)	438	WP_010906313.1	zinc-dependent MarR family transcriptional regulator	-
EFD52_RS09975 (EFD52_09975)	comGG	2027964..2028248 (-)	285	WP_010906314.1	competence type IV pilus minor pilin ComGG	Machinery gene
EFD52_RS09980 (EFD52_09980)	comGF	2028287..2028733 (-)	447	WP_164752712.1	competence type IV pilus minor pilin ComGF	Machinery gene
EFD52_RS09985 (EFD52_09985)	comGE	2028696..2028992 (-)	297	WP_010906316.1	competence type IV pilus minor pilin ComGE	Machinery gene
EFD52_RS09990 (EFD52_09990)	comGD	2028964..2029362 (-)	399	WP_021214886.1	competence type IV pilus minor pilin ComGD	Machinery gene
EFD52_RS09995 (EFD52_09995)	comGC	2029355..2029738 (-)	384	WP_010906318.1	competence type IV pilus major pilin ComGC	Machinery gene
EFD52_RS10000 (EFD52_10000)	comGB	2029752..2030825 (-)	1074	WP_010906319.1	competence type IV pilus assembly protein ComGB	Machinery gene
EFD52_RS10005 (EFD52_10005)	comGA	2030719..2031657 (-)	939	WP_010906320.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 17083.85 Da
• Isoelectric Point: 8.6780

>NTDB_id=324338 EFD52_RS09980 WP_164752712.1 2028287..2028733(-) (comGF) [Lactococcus lactis strain IL6288]
MERKLCDLNFKVKAFTLLECLVALLAISGSALVISGLTKMLKEQVALSQSDSIKDWQIFCQQMRFELSGTKLDRVEQNFL
YVTKDKKLRFGFMSDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide

• Length: 447 bp

>NTDB_id=324338 EFD52_RS09980 WP_164752712.1 2028287..2028733(-) (comGF) [Lactococcus lactis strain IL6288]
ATGGAAAGGAAATTATGCGACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGCCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCGCTTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAACAGAATTTTCTG
TATGTAACTAAAGATAAAAAACTAAGGTTTGGTTTTATGAGTGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAAAGAACATTTATTTATCAATTTCCAGAAGATACGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (46-137)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	74.82	93.919	0.703
comYF	Streptococcus mutans UA140	47.518	95.27	0.453
comYF	Streptococcus mutans UA159	46.809	95.27	0.446
comGF/cglF	Streptococcus mitis SK321	43.066	92.568	0.399
comGF/cglF	Streptococcus pneumoniae Rx1	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae D39	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae R6	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae TIGR4	40.426	95.27	0.385
comGF/cglF	Streptococcus mitis NCTC 12261	41.606	92.568	0.385