Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   EEA47_RS07485 Genome accession   NZ_CP033457
Coordinates   1573220..1574965 (-) Length   581 a.a.
NCBI ID   WP_213908265.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain SNU_SP1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1568220..1579965
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EEA47_RS07465 (EEA47_07735) - 1568909..1569748 (-) 840 WP_005382762.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  EEA47_RS07470 (EEA47_07740) - 1569818..1571329 (-) 1512 WP_213908266.1 SPOR domain-containing protein -
  EEA47_RS07475 (EEA47_07745) aroB 1571382..1572482 (-) 1101 WP_005381318.1 3-dehydroquinate synthase -
  EEA47_RS07480 (EEA47_07750) aroK 1572514..1573032 (-) 519 WP_005381319.1 shikimate kinase AroK -
  EEA47_RS07485 (EEA47_07755) pilQ 1573220..1574965 (-) 1746 WP_213908265.1 type IV pilus secretin PilQ Machinery gene
  EEA47_RS07490 (EEA47_07760) pilP 1574991..1575506 (-) 516 WP_005382766.1 pilus assembly protein PilP Machinery gene
  EEA47_RS07495 (EEA47_07765) pilO 1575496..1576089 (-) 594 WP_064354357.1 type 4a pilus biogenesis protein PilO Machinery gene
  EEA47_RS07500 (EEA47_07770) pilN 1576082..1576657 (-) 576 WP_054577259.1 PilN domain-containing protein Machinery gene
  EEA47_RS07505 (EEA47_07775) pilM 1576641..1577657 (-) 1017 WP_017821733.1 type IV pilus biogenesis protein PilM Machinery gene

Sequence

Protein


Download         Length: 581 a.a.        Molecular weight: 63698.72 Da        Isoelectric Point: 4.4329

>NTDB_id=323804 EEA47_RS07485 WP_213908265.1 1573220..1574965(-) (pilQ) [Vibrio alginolyticus strain SNU_SP1]
MKMRQGLTRTPRILTGLFSLWLFVFLAPLAAAEEVSSNALKSIDFRTNKDKDAVIVVELASPAAIVDIKRVQEGLSIDLL
NTSVKDEQLYLLDVKDFSTVVESVEVFRDTSTTRLVANINEEYMHDYRLTGRFLEIKVAKIKPNEKAPDKSILEREGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQIKGLDKRVDGNVILVAPTDELDLREKQQ
LEKQKLTEEMGELSSEIIKVNFAKASDIAEMINGEGNISMLSERGSMTIDERTNSLLIRELPENIEVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGIRWGFTSINGSNTVGGSIENNLATIGLYNGEGGDDGGDGDGVGIDDFLNVNLAATSPNAT
SIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVTFKKAVLSLKVTPQI
TPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFRR
SYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1746 bp        

>NTDB_id=323804 EEA47_RS07485 WP_213908265.1 1573220..1574965(-) (pilQ) [Vibrio alginolyticus strain SNU_SP1]
ATGAAAATGAGACAAGGATTAACACGTACACCAAGGATACTTACTGGGCTCTTCTCCTTATGGTTGTTTGTATTTTTAGC
CCCTTTAGCGGCAGCTGAAGAAGTTTCGTCCAATGCCCTAAAAAGTATCGACTTTCGAACTAATAAAGATAAAGACGCCG
TGATTGTCGTCGAATTGGCGTCACCGGCTGCGATTGTAGATATCAAGCGTGTACAGGAAGGGTTAAGTATCGATTTGCTT
AACACTTCAGTAAAAGACGAGCAATTGTATCTCCTAGACGTGAAAGATTTTTCGACTGTGGTTGAGAGCGTAGAAGTGTT
TCGCGACACCTCGACGACGCGCTTGGTTGCGAACATCAATGAAGAATACATGCATGATTACCGTTTAACGGGGCGTTTTT
TGGAAATCAAAGTAGCGAAGATCAAACCCAATGAGAAAGCCCCAGATAAAAGCATTTTAGAAAGAGAAGGTAAGCTTATT
TCGATTAACTTCCAAGATATTCCTGTGCGAAATGTCTTGCAGCTTATTGCTGATTACAACCAATTTAATTTGGTTGTCTC
CGACTCTGTCGAAGGCAACCTCACGCTTCGTCTTGATGGTGTCCCTTGGCAGCAAGTTCTGGATATTATTTTGCAAATTA
AGGGGTTAGATAAGCGTGTTGATGGCAACGTGATTTTAGTTGCTCCGACTGATGAGCTGGACTTGCGTGAGAAGCAGCAG
TTAGAAAAACAAAAACTGACGGAAGAAATGGGAGAGCTTTCATCTGAAATCATCAAAGTTAATTTTGCTAAAGCATCAGA
CATTGCGGAGATGATCAATGGTGAAGGAAATATCAGCATGCTGTCAGAGCGCGGCAGTATGACCATTGATGAGCGAACTA
ACTCGTTATTGATTAGAGAGTTACCTGAAAACATCGAGGTGATTCGAGAAATTATTGAGTCTTTGGATATTCCAGTTAAA
CAGGTTCAAATTGAGGCGCGTATTGTGACCATCAATGAAGGTAACATGGACGAGCTAGGTATTCGTTGGGGTTTTACGTC
TATTAATGGCAGTAATACAGTGGGCGGTTCTATTGAGAACAACCTTGCCACGATCGGTCTCTATAATGGAGAAGGGGGTG
ATGACGGTGGTGACGGTGATGGAGTAGGGATCGATGACTTCCTAAATGTAAACTTAGCTGCAACCAGTCCGAATGCCACC
AGCATTGCTTTTCAGGTCGCAAAGTTAGGTTCAGACACCTTGTTGGATCTTGAGCTTTCTGCTCTGCAACAAGAGTCTAA
AGCTGAGATTATCTCGAGCCCACGTTTGATCACTACCAATAAGAAACCTGCCTATATCGAACAAGGTACAGAGATTCCTT
ACCTAGAGTCATCATCAAGTGGTGCAACGACGGTGACGTTCAAAAAGGCGGTATTAAGTTTGAAGGTAACCCCGCAAATC
ACACCGGATAACCGCTTAGTATTGGATTTAAGTGTCACGCAAGATAGACCAGGACAAGTCGTGAAGACAGGCACTGGTGA
AGCTGTTGCGATTGATACTCAACGTATCGGGACGCAAGTGTTAGTGAATAACGGTGAAACGGTCGTTCTTGGTGGTATTT
TTCAGCACAGCATTACTAACTCGGTGGACAAAGTGCCTTTGTTGGGCGATCTTCCGCTATTAGGTGCGCTTTTTCGTCGA
AGTTATGAAAATGTTGGTAAAAGTGAGCTGTTAATTTTCGTCACGCCTAAGGTGGTGATTCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)

Bacterial type II and III secretion system protein (Secretin)

(421-579)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

92.771

100

0.928
  pilQ Vibrio cholerae strain A1552

74.57

100

0.747
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

74.57

100

0.747
  pilQ Pseudomonas aeruginosa PAK

39.896

99.656

0.398
  pilQ Legionella pneumophila strain Lp02

40.608

96.213

0.391
  pilQ Legionella pneumophila strain ERS1305867

40.608

96.213

0.391
  comQ Acinetobacter baylyi ADP1

37.801

100

0.379
  pilQ Acinetobacter baumannii D1279779

35.953

100

0.37
  pilQ Acinetobacter baumannii strain A118

35.953

100

0.37

Multiple sequence alignment