Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   EEA47_RS07500 Genome accession   NZ_CP033457
Coordinates   1576082..1576657 (-) Length   191 a.a.
NCBI ID   WP_054577259.1    Uniprot ID   A0AAW6ZZY0
Organism   Vibrio alginolyticus strain SNU_SP1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1571082..1581657
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EEA47_RS07475 (EEA47_07745) aroB 1571382..1572482 (-) 1101 WP_005381318.1 3-dehydroquinate synthase -
  EEA47_RS07480 (EEA47_07750) aroK 1572514..1573032 (-) 519 WP_005381319.1 shikimate kinase AroK -
  EEA47_RS07485 (EEA47_07755) pilQ 1573220..1574965 (-) 1746 WP_213908265.1 type IV pilus secretin PilQ Machinery gene
  EEA47_RS07490 (EEA47_07760) pilP 1574991..1575506 (-) 516 WP_005382766.1 pilus assembly protein PilP Machinery gene
  EEA47_RS07495 (EEA47_07765) pilO 1575496..1576089 (-) 594 WP_064354357.1 type 4a pilus biogenesis protein PilO Machinery gene
  EEA47_RS07500 (EEA47_07770) pilN 1576082..1576657 (-) 576 WP_054577259.1 PilN domain-containing protein Machinery gene
  EEA47_RS07505 (EEA47_07775) pilM 1576641..1577657 (-) 1017 WP_017821733.1 type IV pilus biogenesis protein PilM Machinery gene
  EEA47_RS07510 (EEA47_07780) - 1577802..1580363 (+) 2562 WP_025768081.1 penicillin-binding protein 1A -
  EEA47_RS07515 (EEA47_07785) oxyR 1580417..1581328 (-) 912 WP_005381326.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 191 a.a.        Molecular weight: 22362.86 Da        Isoelectric Point: 7.4657

>NTDB_id=323807 EEA47_RS07500 WP_054577259.1 1576082..1576657(-) (pilN) [Vibrio alginolyticus strain SNU_SP1]
MLYRINLLPWRENQREEHRRRFISLVALGMLVAVGIQWGIGKFFEYQQDQQQDRLDYLTHYIAELDQRIEAMKIAEQEHS
KILERLKVVEGLQNGRNKTTEFMNLMPAVIPEGVYVDKIKMNDLEIEISGISDSTPKLATMLDNMERSAKLNDVEMHSIV
HGKARFGKEFQTFKVSFMFKVTEKKLEAQRG

Nucleotide


Download         Length: 576 bp        

>NTDB_id=323807 EEA47_RS07500 WP_054577259.1 1576082..1576657(-) (pilN) [Vibrio alginolyticus strain SNU_SP1]
GTGCTTTATCGCATTAATTTACTTCCTTGGCGAGAGAATCAAAGAGAAGAGCACCGACGTCGTTTCATCAGTTTGGTCGC
ATTAGGCATGTTGGTCGCTGTTGGCATCCAGTGGGGTATTGGAAAGTTTTTTGAGTACCAACAAGATCAACAACAGGATC
GGCTCGATTACCTCACTCACTACATTGCCGAACTCGACCAGCGTATTGAAGCAATGAAAATTGCAGAGCAAGAGCACAGC
AAAATCCTCGAACGCCTAAAGGTCGTTGAAGGTTTACAAAACGGACGAAACAAAACGACCGAGTTCATGAACTTAATGCC
TGCCGTTATCCCTGAAGGTGTATATGTCGACAAGATCAAAATGAATGATCTGGAAATTGAGATCTCTGGGATCTCTGATA
GCACTCCAAAACTTGCCACGATGCTGGACAACATGGAGCGCTCGGCCAAGTTGAACGACGTAGAAATGCATTCCATCGTT
CACGGCAAAGCCCGTTTTGGGAAAGAGTTTCAAACATTCAAAGTGTCTTTCATGTTCAAAGTAACGGAGAAGAAATTGGA
GGCTCAACGTGGTTGA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

82.723

100

0.827
  pilN Vibrio cholerae strain A1552

62.712

92.67

0.581

Multiple sequence alignment