Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   EEA47_RS07505 Genome accession   NZ_CP033457
Coordinates   1576641..1577657 (-) Length   338 a.a.
NCBI ID   WP_017821733.1    Uniprot ID   A0AAW7A029
Organism   Vibrio alginolyticus strain SNU_SP1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1571641..1582657
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EEA47_RS07480 (EEA47_07750) aroK 1572514..1573032 (-) 519 WP_005381319.1 shikimate kinase AroK -
  EEA47_RS07485 (EEA47_07755) pilQ 1573220..1574965 (-) 1746 WP_213908265.1 type IV pilus secretin PilQ Machinery gene
  EEA47_RS07490 (EEA47_07760) pilP 1574991..1575506 (-) 516 WP_005382766.1 pilus assembly protein PilP Machinery gene
  EEA47_RS07495 (EEA47_07765) pilO 1575496..1576089 (-) 594 WP_064354357.1 type 4a pilus biogenesis protein PilO Machinery gene
  EEA47_RS07500 (EEA47_07770) pilN 1576082..1576657 (-) 576 WP_054577259.1 PilN domain-containing protein Machinery gene
  EEA47_RS07505 (EEA47_07775) pilM 1576641..1577657 (-) 1017 WP_017821733.1 type IV pilus biogenesis protein PilM Machinery gene
  EEA47_RS07510 (EEA47_07780) - 1577802..1580363 (+) 2562 WP_025768081.1 penicillin-binding protein 1A -
  EEA47_RS07515 (EEA47_07785) oxyR 1580417..1581328 (-) 912 WP_005381326.1 DNA-binding transcriptional regulator OxyR -
  EEA47_RS07520 (EEA47_07790) - 1581498..1582226 (+) 729 WP_005382781.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37595.95 Da        Isoelectric Point: 7.2224

>NTDB_id=323808 EEA47_RS07505 WP_017821733.1 1576641..1577657(-) (pilM) [Vibrio alginolyticus strain SNU_SP1]
MDKLIVTGIDIGHNSLKAVVLKPTGNNYALLGYKEILLKEGIVAENNTINHQEIVKTLKQIKKELPFGAKKVAISVPDNS
VISKKLQIEQNLEESEVEFAVIQAFSHQSPFPVEELSLDFVRLRAEGGQSGSDSYQVFATRKDVVEGRVDALQKSGLKPV
LVDVHSQSLGHIWKLTADRFPDKNKYCLLDIGTLSSSFTMFTEQGELFHKEFAYGTRGANSASPVDFLSDDAQEKTELFN
RQIIERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSQQLSLECELLNPLGLFEMKVSKRKRRAVDWQHFSTAA
GLAVRGIHWLGGARALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=323808 EEA47_RS07505 WP_017821733.1 1576641..1577657(-) (pilM) [Vibrio alginolyticus strain SNU_SP1]
ATGGATAAGCTAATCGTTACCGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCACAGGCAACAA
TTACGCTCTGTTGGGTTACAAAGAAATATTACTAAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTCAAGCAGATAAAAAAAGAACTGCCTTTTGGTGCTAAAAAAGTTGCGATCTCAGTTCCTGATAACTCG
GTAATTAGCAAGAAACTGCAAATAGAACAAAATCTTGAAGAGAGTGAAGTTGAGTTTGCAGTGATACAAGCCTTTTCTCA
TCAATCGCCATTCCCTGTCGAAGAGCTGAGTTTAGACTTCGTTCGTCTTCGCGCTGAGGGCGGGCAGAGTGGCAGTGATA
GCTATCAAGTGTTTGCGACAAGAAAAGATGTGGTAGAAGGCCGAGTCGATGCATTACAAAAGTCCGGACTAAAACCTGTT
TTAGTGGACGTGCATTCGCAAAGCCTTGGTCATATCTGGAAGCTCACCGCCGATCGCTTTCCTGACAAAAACAAATACTG
CTTACTCGATATTGGCACCTTATCGAGCTCCTTCACCATGTTTACCGAACAAGGTGAACTGTTTCACAAAGAGTTTGCTT
ACGGCACACGCGGAGCAAACAGCGCTTCTCCAGTCGATTTTCTGTCCGATGATGCTCAGGAGAAAACAGAGCTGTTTAAC
CGTCAAATCATAGAGCGAGTAAAGCGCCAGATGCAGCTCTATACCTCAATCAATGGCTCACAAAACATCAAGGGTATCTG
GTTATCAGGGGAGGGGGCCTCCACTCCCATGCTCGCAGAAGAGTTGTCTCAACAATTGTCGTTAGAGTGTGAATTGCTAA
ATCCGCTAGGGCTATTTGAGATGAAGGTGTCTAAGCGTAAGCGTCGTGCTGTGGATTGGCAGCATTTCTCAACAGCAGCC
GGACTGGCCGTTCGTGGCATTCATTGGCTAGGAGGCGCCCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

82.84

100

0.828
  pilM Vibrio cholerae strain A1552

50.448

99.112

0.5

Multiple sequence alignment