Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   D7I46_RS06705 Genome accession   NZ_CP032627
Coordinates   1436553..1437209 (-) Length   218 a.a.
NCBI ID   WP_120772199.1    Uniprot ID   A0A387BQP9
Organism   Lactococcus allomyrinae strain 1JSPR-7     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1431553..1442209
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D7I46_RS06685 (D7I46_06685) atpB 1431931..1432644 (-) 714 WP_120772196.1 F0F1 ATP synthase subunit A -
  D7I46_RS06690 (D7I46_06690) - 1432690..1432896 (-) 207 WP_120772197.1 F0F1 ATP synthase subunit C -
  D7I46_RS06695 (D7I46_06695) - 1433135..1433917 (-) 783 WP_120773311.1 alpha/beta hydrolase -
  D7I46_RS06700 (D7I46_06700) comEC 1434329..1436572 (-) 2244 WP_120772198.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  D7I46_RS06705 (D7I46_06705) comEA 1436553..1437209 (-) 657 WP_120772199.1 helix-hairpin-helix domain-containing protein Machinery gene
  D7I46_RS06710 (D7I46_06710) - 1437321..1438712 (-) 1392 WP_120772200.1 ABC transporter permease -
  D7I46_RS06715 (D7I46_06715) - 1438709..1439665 (-) 957 WP_120772201.1 ABC transporter ATP-binding protein -
  D7I46_RS06720 (D7I46_06720) - 1439924..1440451 (-) 528 WP_120772202.1 hypothetical protein -
  D7I46_RS06725 (D7I46_06725) - 1440452..1440652 (-) 201 WP_120772203.1 helix-turn-helix transcriptional regulator -
  D7I46_RS06730 (D7I46_06730) - 1440771..1441178 (-) 408 WP_120772204.1 hypothetical protein -
  D7I46_RS06735 (D7I46_06735) - 1441354..1441914 (-) 561 WP_120772205.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 218 a.a.        Molecular weight: 23296.19 Da        Isoelectric Point: 4.7215

>NTDB_id=317304 D7I46_RS06705 WP_120772199.1 1436553..1437209(-) (comEA) [Lactococcus allomyrinae strain 1JSPR-7]
MERIIEFIKENLKLVILSVVALFAGGIFYFVSHPKSADNALSATMLSSVSTDKTSVSTSNHFSKATEIEVDLKGAVVKPN
VYNVSSDMRVDDLIKLAGGFTTNADQNQVNLAAKLKDEEVVYVPQKGEMASGASADNSVSTDGVGNSLTTTSQTPKVNIN
TADLTQLQTLNGIGQKKAQDIIDYRTQNGNFQSVEDLGNVPGFGDKTIAKLKDSICVD

Nucleotide


Download         Length: 657 bp        

>NTDB_id=317304 D7I46_RS06705 WP_120772199.1 1436553..1437209(-) (comEA) [Lactococcus allomyrinae strain 1JSPR-7]
ATGGAAAGAATAATCGAATTTATCAAAGAAAATTTGAAACTTGTGATTCTGTCAGTTGTTGCACTGTTTGCTGGTGGAAT
CTTTTATTTTGTCAGTCATCCAAAAAGTGCAGACAATGCCTTGTCAGCAACGATGTTGAGTTCTGTCAGTACTGACAAAA
CTTCTGTCAGCACATCAAACCATTTTTCAAAAGCAACAGAAATTGAAGTTGACCTCAAAGGGGCAGTTGTGAAACCCAAT
GTCTATAATGTCTCTAGCGATATGCGTGTTGATGATTTGATTAAATTGGCTGGAGGTTTTACTACAAATGCGGATCAAAA
TCAGGTTAATTTAGCAGCGAAATTAAAGGATGAAGAGGTAGTTTATGTTCCTCAAAAGGGAGAAATGGCAAGCGGAGCAT
CTGCTGATAACTCTGTCAGTACTGACGGAGTGGGAAATTCGCTGACAACCACATCACAAACGCCAAAAGTAAACATCAAC
ACTGCCGATTTGACACAACTCCAAACTTTGAACGGTATTGGTCAAAAGAAGGCGCAAGATATCATTGATTATCGCACTCA
AAATGGTAATTTTCAATCAGTAGAAGATCTAGGAAATGTACCAGGTTTTGGCGATAAAACAATTGCAAAATTAAAGGATT
CTATCTGTGTTGACTAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(153-215)

SLBB domain (SLBB)

(70-123)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A387BQP9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Lactococcus lactis subsp. cremoris KW2

56.364

100

0.569
  comEA/celA/cilE Streptococcus mitis NCTC 12261

41.778

100

0.431
  comEA/celA/cilE Streptococcus pneumoniae Rx1

41.333

100

0.427
  comEA/celA/cilE Streptococcus pneumoniae D39

41.333

100

0.427
  comEA/celA/cilE Streptococcus pneumoniae R6

41.333

100

0.427
  comEA Streptococcus thermophilus LMD-9

38.819

100

0.422
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

40.889

100

0.422
  comEA/celA/cilE Streptococcus mitis SK321

40

100

0.413
  comEA Latilactobacillus sakei subsp. sakei 23K

37.838

100

0.385
  comEA Staphylococcus aureus N315

34.764

100

0.372
  comEA Bacillus subtilis subsp. subtilis str. 168

38.028

97.706

0.372
  comEA Staphylococcus aureus MW2

35.398

100

0.367

Multiple sequence alignment