Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   D7D53_RS08345 Genome accession   NZ_CP032621
Coordinates   1650271..1651212 (-) Length   313 a.a.
NCBI ID   WP_120770674.1    Uniprot ID   A0A387B3U9
Organism   Streptococcus gwangjuensis strain KCOM 1679 (=ChDC B345)     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1645271..1656212
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D7D53_RS08305 (D7D53_08305) comYH 1645880..1646833 (-) 954 WP_120770670.1 class I SAM-dependent methyltransferase Machinery gene
  D7D53_RS08310 (D7D53_08310) - 1646894..1647481 (-) 588 WP_162927893.1 class I SAM-dependent methyltransferase -
  D7D53_RS08315 (D7D53_08315) comGG/cglG 1647514..1647915 (-) 402 WP_120770671.1 competence type IV pilus minor pilin ComGG Machinery gene
  D7D53_RS08320 (D7D53_08320) comGF/cglF 1647893..1648354 (-) 462 WP_000250521.1 competence type IV pilus minor pilin ComGF Machinery gene
  D7D53_RS08325 (D7D53_08325) comGE/cglE 1648317..1648619 (-) 303 WP_281268606.1 competence type IV pilus minor pilin ComGE Machinery gene
  D7D53_RS08330 (D7D53_08330) comGD/cglD 1648582..1648986 (-) 405 WP_245941793.1 competence type IV pilus minor pilin ComGD Machinery gene
  D7D53_RS08335 (D7D53_08335) comGC/cglC 1648979..1649305 (-) 327 WP_045606658.1 competence type IV pilus major pilin ComGC Machinery gene
  D7D53_RS08340 (D7D53_08340) comGB/cglB 1649307..1650323 (-) 1017 WP_120770673.1 competence type IV pilus assembly protein ComGB Machinery gene
  D7D53_RS08345 (D7D53_08345) comGA/cglA/cilD 1650271..1651212 (-) 942 WP_120770674.1 competence type IV pilus ATPase ComGA Machinery gene
  D7D53_RS08350 (D7D53_08350) - 1651288..1651653 (-) 366 WP_120770675.1 DUF1033 family protein -
  D7D53_RS08355 (D7D53_08355) - 1651805..1652863 (-) 1059 WP_120770676.1 zinc-dependent alcohol dehydrogenase family protein -
  D7D53_RS08360 (D7D53_08360) pbp1b 1653333..1655783 (-) 2451 WP_120770677.1 penicillin-binding protein PBP1B -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35590.58 Da        Isoelectric Point: 5.8494

>NTDB_id=317220 D7D53_RS08345 WP_120770674.1 1650271..1651212(-) (comGA/cglA/cilD) [Streptococcus gwangjuensis strain KCOM 1679 (=ChDC B345)]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIGCYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYE
YDQKMASLRLSTVGDYRGHESLVIRLLHDEKQDLHFWFQDIDELGKQYRQRGLYLFAGPVGSGKTTLMHELAKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVIRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFASKDYQDHQPTSWNEQIDQLLKDGYITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=317220 D7D53_RS08345 WP_120770674.1 1650271..1651212(-) (comGA/cglA/cilD) [Streptococcus gwangjuensis strain KCOM 1679 (=ChDC B345)]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCAGCTCGGAAAAAAGGGGCGCAAGATATTTATTTTGTCCCTAAGTT
AGACGCTTATGAGCTTCATATGAGGGTAGGAGACGAGCGATGTAAAATTGGTTGCTATGATTTTGAAAAGTTTGCGGCCG
TTATTAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAGCGACGTAGTCAACTTGGTTCCTGTGATTATGAA
TATGACCAGAAGATGGCGTCCCTACGTTTATCTACAGTAGGCGATTATAGAGGGCATGAGAGTTTAGTTATTCGCTTGCT
GCATGATGAGAAACAGGACTTGCATTTTTGGTTTCAGGATATTGATGAATTGGGCAAGCAGTACAGGCAACGTGGTCTCT
ATCTCTTTGCTGGTCCAGTCGGAAGTGGCAAGACAACCCTGATGCATGAATTGGCCAAGTCTCTCTTTAAAGGACAGCAA
GTCATGTCCATCGAAGATCCTGTAGAAATCAAGCAGGACGACATGCTCCAATTACAGTTGAATGAAGCGATCGGACTAAC
CTATGAAAATCTCATCAAACTTTCCCTACGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGAGACAGCGAAACGG
CGCGTGCAGTGATCAGAGCCAGTTTGACAGGGGCGACAGTCTTTTCAACCATTCATGCCAAGAGTATCCGAGGTGTTTAT
GAGCGCTTGCTGGAGTTGGGTGTGAGTGAGGAGGAATTAGCAGTCGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAGCAAAGACTATCAAGACCACCAGCCAACTAGCTGGAATGAACAGATTGACCAGC
TTCTTAAAGATGGATATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A387B3U9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

97.125

100

0.971
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

95.208

100

0.952
  comGA/cglA/cilD Streptococcus pneumoniae D39

95.208

100

0.952
  comGA/cglA/cilD Streptococcus pneumoniae R6

95.208

100

0.952
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

95.208

100

0.952
  comYA Streptococcus gordonii str. Challis substr. CH1

78.065

99.042

0.773
  comYA Streptococcus mutans UA159

66.559

99.361

0.661
  comYA Streptococcus mutans UA140

66.559

99.361

0.661
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.581

99.042

0.62
  comGA Lactococcus lactis subsp. cremoris KW2

55.769

99.681

0.556
  comGA Latilactobacillus sakei subsp. sakei 23K

41.912

86.901

0.364

Multiple sequence alignment