Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   D7D53_RS08320 Genome accession   NZ_CP032621
Coordinates   1647893..1648354 (-) Length   153 a.a.
NCBI ID   WP_000250521.1    Uniprot ID   A0A387AZ50
Organism   Streptococcus gwangjuensis strain KCOM 1679 (=ChDC B345)     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1642893..1653354
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D7D53_RS08290 (D7D53_08290) - 1643320..1644144 (-) 825 WP_218961675.1 membrane protein insertase YidC -
  D7D53_RS08295 (D7D53_08295) rnpA 1644119..1644490 (-) 372 WP_000739243.1 ribonuclease P protein component -
  D7D53_RS08300 (D7D53_08300) - 1644639..1645829 (-) 1191 WP_120770669.1 acetate kinase -
  D7D53_RS08305 (D7D53_08305) comYH 1645880..1646833 (-) 954 WP_120770670.1 class I SAM-dependent methyltransferase Machinery gene
  D7D53_RS08310 (D7D53_08310) - 1646894..1647481 (-) 588 WP_162927893.1 class I SAM-dependent methyltransferase -
  D7D53_RS08315 (D7D53_08315) comGG/cglG 1647514..1647915 (-) 402 WP_120770671.1 competence type IV pilus minor pilin ComGG Machinery gene
  D7D53_RS08320 (D7D53_08320) comGF/cglF 1647893..1648354 (-) 462 WP_000250521.1 competence type IV pilus minor pilin ComGF Machinery gene
  D7D53_RS08325 (D7D53_08325) comGE/cglE 1648317..1648619 (-) 303 WP_281268606.1 competence type IV pilus minor pilin ComGE Machinery gene
  D7D53_RS08330 (D7D53_08330) comGD/cglD 1648582..1648986 (-) 405 WP_245941793.1 competence type IV pilus minor pilin ComGD Machinery gene
  D7D53_RS08335 (D7D53_08335) comGC/cglC 1648979..1649305 (-) 327 WP_045606658.1 competence type IV pilus major pilin ComGC Machinery gene
  D7D53_RS08340 (D7D53_08340) comGB/cglB 1649307..1650323 (-) 1017 WP_120770673.1 competence type IV pilus assembly protein ComGB Machinery gene
  D7D53_RS08345 (D7D53_08345) comGA/cglA/cilD 1650271..1651212 (-) 942 WP_120770674.1 competence type IV pilus ATPase ComGA Machinery gene
  D7D53_RS08350 (D7D53_08350) - 1651288..1651653 (-) 366 WP_120770675.1 DUF1033 family protein -
  D7D53_RS08355 (D7D53_08355) - 1651805..1652863 (-) 1059 WP_120770676.1 zinc-dependent alcohol dehydrogenase family protein -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 17869.45 Da        Isoelectric Point: 8.8053

>NTDB_id=317215 D7D53_RS08320 WP_000250521.1 1647893..1648354(-) (comGF/cglF) [Streptococcus gwangjuensis strain KCOM 1679 (=ChDC B345)]
MVQNSCWQSKSHKVKAFTLLESLIALIVISGSLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGN
RLYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVRFRFQFQKGLEREFIYRVEKEKS

Nucleotide


Download         Length: 462 bp        

>NTDB_id=317215 D7D53_RS08320 WP_000250521.1 1647893..1648354(-) (comGF/cglF) [Streptococcus gwangjuensis strain KCOM 1679 (=ChDC B345)]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTTAAGGCTTTCACCTTGTTGGAATCTCTGATTGCTCTCAT
TGTCATCAGTGGGAGTTTACTTCTCTTTCAAGCCATGAGTCAGCTCCTTATTTCAGAAGTTCGCTACCAGCAGCAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAGCTGGAGGCAGAATTAGATCGTTCGCAGTTCGAAAAAGTGGAGGGCAAT
CGCCTCTATATGAAGCAGGATGGCAAGGATATCGCTATAGGTAAGTCTAAATCGGATGATTTTCGGAAAACCGATGCCAG
TGGACGGGGTTATCAGCCTATGGTTTATGGACTCAAATCTGCGCAGATTACAGAGGACAATCAACTGGTTCGTTTTCGTT
TCCAGTTCCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A387AZ50

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus mitis SK321

96.078

100

0.961
  comGF/cglF Streptococcus mitis NCTC 12261

94.771

100

0.948
  comGF/cglF Streptococcus pneumoniae Rx1

94.771

100

0.948
  comGF/cglF Streptococcus pneumoniae D39

94.771

100

0.948
  comGF/cglF Streptococcus pneumoniae R6

94.771

100

0.948
  comGF/cglF Streptococcus pneumoniae TIGR4

94.771

100

0.948
  comGF Lactococcus lactis subsp. cremoris KW2

40

91.503

0.366

Multiple sequence alignment