Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   D7D53_RS08340 Genome accession   NZ_CP032621
Coordinates   1649307..1650323 (-) Length   338 a.a.
NCBI ID   WP_120770673.1    Uniprot ID   A0A387B2C8
Organism   Streptococcus gwangjuensis strain KCOM 1679 (=ChDC B345)     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1644307..1655323
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D7D53_RS08300 (D7D53_08300) - 1644639..1645829 (-) 1191 WP_120770669.1 acetate kinase -
  D7D53_RS08305 (D7D53_08305) comYH 1645880..1646833 (-) 954 WP_120770670.1 class I SAM-dependent methyltransferase Machinery gene
  D7D53_RS08310 (D7D53_08310) - 1646894..1647481 (-) 588 WP_162927893.1 class I SAM-dependent methyltransferase -
  D7D53_RS08315 (D7D53_08315) comGG/cglG 1647514..1647915 (-) 402 WP_120770671.1 competence type IV pilus minor pilin ComGG Machinery gene
  D7D53_RS08320 (D7D53_08320) comGF/cglF 1647893..1648354 (-) 462 WP_000250521.1 competence type IV pilus minor pilin ComGF Machinery gene
  D7D53_RS08325 (D7D53_08325) comGE/cglE 1648317..1648619 (-) 303 WP_281268606.1 competence type IV pilus minor pilin ComGE Machinery gene
  D7D53_RS08330 (D7D53_08330) comGD/cglD 1648582..1648986 (-) 405 WP_245941793.1 competence type IV pilus minor pilin ComGD Machinery gene
  D7D53_RS08335 (D7D53_08335) comGC/cglC 1648979..1649305 (-) 327 WP_045606658.1 competence type IV pilus major pilin ComGC Machinery gene
  D7D53_RS08340 (D7D53_08340) comGB/cglB 1649307..1650323 (-) 1017 WP_120770673.1 competence type IV pilus assembly protein ComGB Machinery gene
  D7D53_RS08345 (D7D53_08345) comGA/cglA/cilD 1650271..1651212 (-) 942 WP_120770674.1 competence type IV pilus ATPase ComGA Machinery gene
  D7D53_RS08350 (D7D53_08350) - 1651288..1651653 (-) 366 WP_120770675.1 DUF1033 family protein -
  D7D53_RS08355 (D7D53_08355) - 1651805..1652863 (-) 1059 WP_120770676.1 zinc-dependent alcohol dehydrogenase family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38379.36 Da        Isoelectric Point: 9.5924

>NTDB_id=317219 D7D53_RS08340 WP_120770673.1 1649307..1650323(-) (comGB/cglB) [Streptococcus gwangjuensis strain KCOM 1679 (=ChDC B345)]
MDISQVFRLKRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVAKMRTGLSQGKSFSEMMEGLGFSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGFVSVLALLALTFYKRSSKMRVFSILARLPFLGIFVQTYLTAYYAREWGNMISQGMELTQIFQIMQEQGSQLFKEI
GQDLAQSLQNGQEFSQTIGTYPFFKKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=317219 D7D53_RS08340 WP_120770673.1 1649307..1650323(-) (comGB/cglB) [Streptococcus gwangjuensis strain KCOM 1679 (=ChDC B345)]
ATGGATATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAAACAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCCAGTGGTTTTCATCTGGTGGAGACCATTTCCTTTTTAGATAGGAGTGCCTTGTTGGACAAGCAGTGCG
TGGCCAAGATGCGCACGGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAGGTTTGGGATTTTCAAGTGCCATT
GTCACTCAGTTATCCCTAGCGGAAGTCCATGGAAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTAGACAATCT
GGCCAAAGTCAAGAAAAAATTAATTGAAGTAGCGACCTATCCCTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGTAATTACCTACTACCACAACTGGATAGTAGCAATATTGCCACTCAAATTATCGGTAATCTGCCACAAATCTTT
CTGGGTATGGTAGGGTTTGTTTCCGTGCTTGCCCTTTTAGCATTAACTTTCTATAAAAGAAGTTCTAAGATGCGCGTCTT
TTCTATCTTAGCACGCCTTCCCTTTCTTGGAATTTTTGTGCAGACCTATTTGACAGCCTATTATGCGCGTGAATGGGGGA
ATATGATTTCGCAGGGAATGGAATTGACGCAGATTTTTCAAATCATGCAAGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGACTTAGCTCAATCCCTACAAAATGGTCAGGAATTTTCTCAAACGATAGGAACCTATCCTTTCTTTAAAAAGGA
GTTGAGTCTCATTATCGAGTATGGGGAAGTTAAGTCCAAGCTGGGGAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTTAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A387B2C8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus mitis NCTC 12261

96.746

100

0.967
  comGB/cglB Streptococcus mitis SK321

96.746

100

0.967
  comGB/cglB Streptococcus pneumoniae Rx1

95.562

100

0.956
  comGB/cglB Streptococcus pneumoniae D39

95.562

100

0.956
  comGB/cglB Streptococcus pneumoniae R6

95.562

100

0.956
  comGB/cglB Streptococcus pneumoniae TIGR4

95.562

100

0.956
  comYB Streptococcus gordonii str. Challis substr. CH1

72.619

99.408

0.722
  comYB Streptococcus mutans UA140

58.228

93.491

0.544
  comYB Streptococcus mutans UA159

58.228

93.491

0.544
  comGB Lactococcus lactis subsp. cremoris KW2

51.198

98.817

0.506

Multiple sequence alignment