Detailed information NTG0293086

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: DJ564_RS18000
• Genome accession: NZ_CP029482
• Coordinates: 3820017..3821126 (-)
• Length: 369 a.a.
• NCBI ID: WP_109632041.1
• Uniprot ID: -
• Organism: Pseudomonas sp. 31-12
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3815017..3826126

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DJ564_RS17980 (DJ564_17980)	-	3815601..3816992 (+)	1392	WP_109632035.1	GntP family permease	-
DJ564_RS17985 (DJ564_17985)	hbdH	3817000..3817773 (+)	774	WP_109632037.1	3-hydroxybutyrate dehydrogenase	-
DJ564_RS17990 (DJ564_17990)	-	3817949..3819904 (+)	1956	WP_109632039.1	acetoacetate--CoA ligase	-
DJ564_RS18000 (DJ564_18000)	pilU	3820017..3821126 (-)	1110	WP_109632041.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
DJ564_RS18005 (DJ564_18005)	-	3821248..3823782 (+)	2535	WP_109632043.1	PAS domain-containing protein	-
DJ564_RS18010 (DJ564_18010)	-	3823839..3824114 (+)	276	WP_007898282.1	peptidylprolyl isomerase	-
DJ564_RS18015 (DJ564_18015)	-	3824257..3825189 (-)	933	WP_109632045.1	sugar kinase	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 41132.07 Da
• Isoelectric Point: 6.1928

>NTDB_id=293086 DJ564_RS18000 WP_109632041.1 3820017..3821126(-) (pilU) [Pseudomonas sp. 31-12]
MEIDALLQILADRNGSDLYLSPGAPPSARFDGVLKPLTDQPFKPGEVAAIAESIMDAEQRLEFDRELEMNLAISLAGVGR
FRVNIFKQRNDVSIVARNIKLDIPRFEDLKLPAVLLETVMLKQGLMLFVGATDCGKSTSLAALIDYRNRHSSGHIVTIED
PIEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRI
INFFPEERHTQLLHDLGNNLKAFVSQRLVRTRDGQRRAAVEVMLGTPTIGDLIRRNEFAELKGIMEKSLELGMQTFDGAL
YALVVEGAINEEEALKHADSVNNLRLRLKLHSDASPPTQPPSGEWGLID

Nucleotide

• Length: 1110 bp

>NTDB_id=293086 DJ564_RS18000 WP_109632041.1 3820017..3821126(-) (pilU) [Pseudomonas sp. 31-12]
ATGGAAATCGATGCACTGTTGCAGATACTGGCTGACCGGAACGGATCCGATCTCTATCTCTCCCCCGGCGCGCCACCCAG
TGCACGGTTCGACGGCGTGCTCAAACCGCTGACCGATCAACCGTTCAAACCGGGGGAAGTCGCAGCCATTGCCGAGTCCA
TCATGGACGCCGAACAACGTCTGGAGTTCGATCGCGAACTGGAGATGAACCTGGCGATCTCCCTGGCAGGTGTCGGGCGC
TTTCGGGTCAACATCTTCAAGCAACGCAACGATGTGTCAATCGTGGCACGCAACATCAAGCTCGACATTCCGCGCTTCGA
AGACCTCAAGTTGCCGGCGGTGCTGCTCGAAACCGTGATGCTCAAACAAGGCCTGATGCTGTTCGTCGGCGCCACCGATT
GCGGCAAGTCGACCTCCCTGGCGGCGCTGATCGATTACCGCAACCGTCACAGCAGTGGCCATATCGTCACCATCGAAGAC
CCGATCGAGTACATTCACCGGCACAAGAAGTCGATCATTAACCAGCGTGAGGTCGGCGTCGATACTCGCAGTTTTCATGC
TGCCCTGAAAAACACCCTGCGTCAGGCGCCGGACGTCGTCTTGATCGGCGAAATCCGCGACCGCGAAACCATGGAGCATG
CGCTGGCATTTGCCGATACCGGTCATCTGGTGATTTCCACCTTGCACGCGCACAACGCCAATCAGGCGCTGGACCGCATT
ATCAATTTCTTCCCTGAGGAGCGACATACGCAGCTGCTGCATGATCTGGGCAACAACCTCAAAGCATTCGTGTCCCAACG
ATTGGTGCGTACCCGCGACGGGCAGCGCCGGGCGGCGGTGGAGGTGATGCTGGGGACACCAACCATCGGTGACCTGATCC
GGCGCAATGAGTTTGCCGAGCTGAAGGGGATCATGGAGAAATCGCTGGAATTGGGGATGCAGACGTTTGACGGTGCGCTG
TATGCGTTGGTTGTCGAAGGGGCGATCAATGAGGAGGAAGCGTTGAAGCATGCGGATTCGGTGAACAACTTGCGCTTGCG
GCTGAAGTTGCATAGCGATGCGTCACCGCCGACTCAACCGCCCTCGGGAGAGTGGGGATTAATAGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	57.183	96.206	0.55
pilU	Acinetobacter baylyi ADP1	52.646	97.29	0.512
pilU	Vibrio cholerae strain A1552	51.429	94.851	0.488
pilT	Pseudomonas aeruginosa PAK	40.698	93.225	0.379
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.742	90.244	0.377
pilT	Vibrio cholerae strain A1552	41.742	90.244	0.377
pilT	Pseudomonas stutzeri DSM 10701	40.407	93.225	0.377
pilT	Legionella pneumophila strain Lp02	41.018	90.515	0.371
pilT	Legionella pneumophila strain ERS1305867	41.018	90.515	0.371
pilT	Neisseria gonorrhoeae MS11	38.551	93.496	0.36
pilT	Neisseria meningitidis 8013	38.551	93.496	0.36
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.233	91.87	0.36