Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DGN21_RS16745 Genome accession   NZ_CP029263
Coordinates   4021943..4023076 (+) Length   377 a.a.
NCBI ID   WP_016900968.1    Uniprot ID   A0A2N7V1C6
Organism   Xanthomonas sp. MLO165     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4016943..4028076
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DGN21_RS16720 (DGN21_17125) - 4017067..4018266 (-) 1200 WP_184379319.1 cystathionine gamma-synthase -
  DGN21_RS16725 (DGN21_17130) - 4018353..4019723 (-) 1371 WP_126966628.1 pyridoxal-phosphate dependent enzyme -
  DGN21_RS16730 (DGN21_17135) - 4020010..4020228 (-) 219 WP_002814034.1 YdcH family protein -
  DGN21_RS16735 (DGN21_17140) - 4020574..4021464 (-) 891 WP_046344413.1 membrane protein -
  DGN21_RS16740 (DGN21_17145) - 4021461..4021844 (-) 384 WP_039525328.1 DUF4398 domain-containing protein -
  DGN21_RS16745 (DGN21_17150) pilU 4021943..4023076 (+) 1134 WP_016900968.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DGN21_RS16750 (DGN21_17155) maiA 4023360..4024025 (-) 666 WP_102580573.1 maleylacetoacetate isomerase -
  DGN21_RS16755 (DGN21_17160) - 4024069..4025127 (-) 1059 WP_026064014.1 fumarylacetoacetate hydrolase family protein -
  DGN21_RS16760 (DGN21_17170) - 4025436..4026824 (+) 1389 WP_039525325.1 DEAD/DEAH box helicase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41754.11 Da        Isoelectric Point: 6.1401

>NTDB_id=291470 DGN21_RS16745 WP_016900968.1 4021943..4023076(+) (pilU) [Xanthomonas sp. MLO165]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALSDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSLRLVKGVDGRRRPATEVLLNTPMIRDLLRRGQVHEVKQAMEESLEEGMQTFDQCL
FRMVKSGEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYETAAAPKISHGFI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=291470 DGN21_RS16745 WP_016900968.1 4021943..4023076(+) (pilU) [Xanthomonas sp. MLO165]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAATGCCTCGGACATGTTCTTGACCACCGGCGCACCGGTGTA
CATCAAGATCGAAGGCAAGCTCTACCCGCTCGGCAATACGGGGTTGCCGCCGGGAATGGTCAAGAAAATCGCTTATTCGT
TGATGGACGAAGGCCAGGTACCGCAGTTCGAGCGCGAGCTGGAGCTCAACATGGCCATTGCCCTGTCCGACGCGGGGCGC
TTCCGCGTGAACGTGTTCAAGCAGCGCGGCGAGGTGGGCATGGTGATCCGCGCCATCCGCAGCCGGATCCCCAGCATCGA
AGAGCTCAACCTGCCGCAGGTGCTCAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTCGGCTCGACCGGCT
CGGGCAAGTCGACCTCGCTGGCCTCGATGATCGACCACCGCAACAGCACCACCACCGGACACATCCTCACCATCGAGGAC
CCGATCGAATATCTGCACAAGCACAAGATGTCGATCGTGAACCAGCGCGAGGTGGGCCTGGACACGCACGCCTTCCAGAG
CGCGCTGAAAAACGCGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGATGCCGAGACGATGGAGGCGG
CGATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACGCTGCACTCCAACAATGCCGACCAGACCATCGAGCGCATC
CTCAATTTCTTCCCCGAGAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGCGCCGTGGTGTCGCTGCG
CCTGGTCAAGGGCGTGGACGGACGCCGCCGGCCGGCAACCGAAGTGCTGCTCAACACGCCGATGATCCGCGACCTGCTGC
GCCGTGGCCAGGTGCACGAGGTCAAGCAGGCGATGGAGGAATCGCTGGAAGAAGGCATGCAGACCTTCGACCAATGCCTG
TTCCGCATGGTCAAGTCCGGCGAAATCGAGCAGGAGGAAGCGCTGCGCGCGGCCGACTCGCGCGATGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAGGGCGGTTCCGGCGAGCACGACCCGTACGCGGATTACGAAACGGCAGCAGCGCCGAAGATCAGCC
ACGGCTTTATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N7V1C6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.089

95.225

0.496
  pilU Vibrio cholerae strain A1552

48.889

95.491

0.467
  pilU Acinetobacter baylyi ADP1

49.153

93.899

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.979

100

0.446
  pilT Pseudomonas aeruginosa PAK

42.308

89.655

0.379
  pilT Legionella pneumophila strain ERS1305867

41.124

89.655

0.369
  pilT Legionella pneumophila strain Lp02

41.124

89.655

0.369
  pilT Acinetobacter baumannii strain A118

40.828

89.655

0.366
  pilT Pseudomonas stutzeri DSM 10701

40.828

89.655

0.366
  pilT Acinetobacter nosocomialis M2

40.828

89.655

0.366
  pilT Acinetobacter baumannii D1279779

40.828

89.655

0.366
  pilT Acinetobacter baylyi ADP1

40.294

90.186

0.363

Multiple sequence alignment