Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LL14B4_RS11860 Genome accession   NZ_CP028160
Coordinates   2376600..2377538 (-) Length   312 a.a.
NCBI ID   WP_033900707.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis strain 14B4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2371600..2382538
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LL14B4_RS11820 (LL14B4_11810) - 2372488..2373330 (-) 843 WP_015427160.1 metal ABC transporter substrate-binding protein -
  LL14B4_RS11825 (LL14B4_11815) - 2373327..2373764 (-) 438 WP_010906313.1 zinc-dependent MarR family transcriptional regulator -
  LL14B4_RS11830 (LL14B4_11820) comGG 2373845..2374129 (-) 285 WP_017865155.1 competence type IV pilus minor pilin ComGG Machinery gene
  LL14B4_RS11835 (LL14B4_11825) comGF 2374168..2374614 (-) 447 WP_058203710.1 competence type IV pilus minor pilin ComGF Machinery gene
  LL14B4_RS11840 (LL14B4_11830) comGE 2374577..2374873 (-) 297 WP_033900709.1 competence type IV pilus minor pilin ComGE Machinery gene
  LL14B4_RS11845 (LL14B4_11835) comGD 2374845..2375243 (-) 399 WP_233610962.1 competence type IV pilus minor pilin ComGD Machinery gene
  LL14B4_RS11850 (LL14B4_11840) comGC 2375236..2375619 (-) 384 WP_080735951.1 competence type IV pilus major pilin ComGC Machinery gene
  LL14B4_RS11855 (LL14B4_11845) comGB 2375633..2376706 (-) 1074 WP_080735950.1 competence type IV pilus assembly protein ComGB Machinery gene
  LL14B4_RS11860 (LL14B4_11850) comGA 2376600..2377538 (-) 939 WP_033900707.1 competence type IV pilus ATPase ComGA Machinery gene

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35691.92 Da        Isoelectric Point: 6.1811

>NTDB_id=281833 LL14B4_RS11860 WP_033900707.1 2376600..2377538(-) (comGA) [Lactococcus lactis subsp. lactis strain 14B4]
MIPKMAKELIQRAIDRDASDIYLIASKEKYKLYFRQMTARVLVEEIGLEVGLALLTHFKFLAGMNTGERRRVQLGACWYE
LEESNAKRLRLSTVGDFEGNESLVIRLLHDQNKELDFWFDEELENYDCKRGLHLFAGPVGSGKTSLMFDLARRHFTDLQV
ITIEEPVELIDSDFIQLQVNEVIGNSYDELIKLSLRHRPDLLIVGEIRDKLTAQAVLRASLTGYTVFSTVHASSVRGVIQ
RLSELGLSHWEIKNALSSVVYQRLIAGKGVLDIAKNEFESWSSENWNKKIDQLLTNGYLTPTEAKREKIKTD

Nucleotide


Download         Length: 939 bp        

>NTDB_id=281833 LL14B4_RS11860 WP_033900707.1 2376600..2377538(-) (comGA) [Lactococcus lactis subsp. lactis strain 14B4]
ATGATACCAAAAATGGCAAAAGAATTAATTCAAAGAGCAATTGATAGAGATGCATCAGATATTTATCTGATTGCTTCAAA
AGAAAAGTATAAACTTTATTTTCGACAAATGACAGCGCGTGTTCTGGTAGAAGAAATTGGGTTAGAAGTTGGCTTAGCAC
TACTTACTCACTTTAAGTTTCTAGCTGGGATGAATACAGGAGAACGCAGACGAGTTCAATTGGGAGCTTGTTGGTATGAA
TTAGAGGAAAGTAATGCAAAACGTTTGCGTCTTTCAACAGTGGGGGATTTCGAGGGTAATGAATCTTTAGTAATACGTCT
CTTGCATGATCAAAACAAAGAACTTGATTTTTGGTTTGATGAAGAGCTAGAGAATTATGATTGTAAAAGAGGATTGCATC
TCTTTGCCGGTCCAGTAGGCTCGGGAAAAACATCTTTAATGTTTGATTTAGCTCGTCGTCATTTTACAGATTTACAAGTT
ATCACTATTGAAGAACCCGTTGAGTTGATTGATTCCGATTTTATTCAGTTACAAGTCAACGAAGTCATTGGTAATAGTTA
TGATGAATTAATTAAATTATCACTGAGGCATCGCCCAGATTTGTTAATCGTTGGTGAAATTCGTGATAAATTGACCGCTC
AAGCAGTTTTACGGGCTAGTTTGACAGGTTATACAGTGTTTTCAACCGTTCATGCCTCTTCTGTTAGAGGTGTCATTCAG
AGATTATCAGAGCTGGGTTTAAGTCATTGGGAAATAAAAAATGCTTTATCAAGTGTTGTTTATCAGCGTTTAATAGCTGG
GAAAGGAGTATTAGACATTGCCAAAAACGAATTTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTAC
TTACAAATGGATATCTCACACCTACTGAAGCCAAAAGGGAAAAAATTAAGACTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

77.244

100

0.772
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

54.984

99.679

0.548
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.376

99.679

0.532
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

52.412

99.679

0.522
  comGA/cglA/cilD Streptococcus pneumoniae D39

52.412

99.679

0.522
  comGA/cglA/cilD Streptococcus pneumoniae R6

52.412

99.679

0.522
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

52.412

99.679

0.522
  comYA Streptococcus mutans UA140

52.244

100

0.522
  comYA Streptococcus mutans UA159

52.244

100

0.522
  comYA Streptococcus gordonii str. Challis substr. CH1

51.757

100

0.519

Multiple sequence alignment