Detailed information NTG0281828

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: LL14B4_RS11835
• Genome accession: NZ_CP028160
• Coordinates: 2374168..2374614 (-)
• Length: 148 a.a.
• NCBI ID: WP_058203710.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis strain 14B4
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2369168..2379614

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LL14B4_RS11800 (LL14B4_11790)	-	2369285..2369662 (-)	378	WP_033900715.1	pyridoxamine 5'-phosphate oxidase family protein	-
LL14B4_RS11805 (LL14B4_11795)	-	2369867..2370733 (+)	867	WP_033900713.1	RluA family pseudouridine synthase	-
LL14B4_RS11810 (LL14B4_11800)	-	2370772..2371581 (-)	810	WP_033900712.1	metal ABC transporter permease	-
LL14B4_RS11815 (LL14B4_11805)	-	2371574..2372311 (-)	738	WP_012898617.1	metal ABC transporter ATP-binding protein	-
LL14B4_RS11820 (LL14B4_11810)	-	2372488..2373330 (-)	843	WP_015427160.1	metal ABC transporter substrate-binding protein	-
LL14B4_RS11825 (LL14B4_11815)	-	2373327..2373764 (-)	438	WP_010906313.1	zinc-dependent MarR family transcriptional regulator	-
LL14B4_RS11830 (LL14B4_11820)	comGG	2373845..2374129 (-)	285	WP_017865155.1	competence type IV pilus minor pilin ComGG	Machinery gene
LL14B4_RS11835 (LL14B4_11825)	comGF	2374168..2374614 (-)	447	WP_058203710.1	competence type IV pilus minor pilin ComGF	Machinery gene
LL14B4_RS11840 (LL14B4_11830)	comGE	2374577..2374873 (-)	297	WP_033900709.1	competence type IV pilus minor pilin ComGE	Machinery gene
LL14B4_RS11845 (LL14B4_11835)	comGD	2374845..2375243 (-)	399	WP_233610962.1	competence type IV pilus minor pilin ComGD	Machinery gene
LL14B4_RS11850 (LL14B4_11840)	comGC	2375236..2375619 (-)	384	WP_080735951.1	competence type IV pilus major pilin ComGC	Machinery gene
LL14B4_RS11855 (LL14B4_11845)	comGB	2375633..2376706 (-)	1074	WP_080735950.1	competence type IV pilus assembly protein ComGB	Machinery gene
LL14B4_RS11860 (LL14B4_11850)	comGA	2376600..2377538 (-)	939	WP_033900707.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 17181.02 Da
• Isoelectric Point: 9.0871

>NTDB_id=281828 LL14B4_RS11835 WP_058203710.1 2374168..2374614(-) (comGF) [Lactococcus lactis subsp. lactis strain 14B4]
MERKLCNLNFKVKAFTLLECLVALLAISGSVLVISGLTKMLKEQVAISQSDSIKDWQIFCQQMRFELSGTKLDRVEQDFL
YVTKDKKLRFGFMRDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide

• Length: 447 bp

>NTDB_id=281828 LL14B4_RS11835 WP_058203710.1 2374168..2374614(-) (comGF) [Lactococcus lactis subsp. lactis strain 14B4]
ATGGAAAGGAAATTATGCAACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGTCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCGATTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAGCAGGATTTTCTG
TATGTAACTAAAGACAAAAAACTAAGGTTTGGTTTTATGAGAGATGATTTCCGGAAGACTGATGACAAAGGCCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAGAGAACATTTATTTATCAATTTCCGGAAGATACGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (48-137)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	75.54	93.919	0.709
comYF	Streptococcus mutans UA140	47.518	95.27	0.453
comYF	Streptococcus mutans UA159	46.809	95.27	0.446
comGF/cglF	Streptococcus mitis SK321	41.606	92.568	0.385
comGF/cglF	Streptococcus mitis NCTC 12261	41.606	92.568	0.385
comGF/cglF	Streptococcus pneumoniae Rx1	39.007	95.27	0.372
comGF/cglF	Streptococcus pneumoniae D39	39.007	95.27	0.372
comGF/cglF	Streptococcus pneumoniae R6	39.007	95.27	0.372
comGF/cglF	Streptococcus pneumoniae TIGR4	39.007	95.27	0.372