Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   C4X49_RS16665 Genome accession   NZ_CP028138
Coordinates   3386599..3387060 (-) Length   153 a.a.
NCBI ID   WP_000993711.1    Uniprot ID   A0A009I3S8
Organism   Acinetobacter baumannii strain NCIMB 8209     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IS/Tn 3384680..3385612 3386599..3387060 flank 987


Gene organization within MGE regions


Location: 3384680..3387060
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C4X49_RS16655 (C4X49_16645) - 3384680..3385612 (+) 933 WP_001043692.1 IS5-like element ISAba13 family transposase -
  C4X49_RS16660 (C4X49_16650) - 3385580..3386542 (-) 963 WP_130089836.1 O-antigen ligase family protein -
  C4X49_RS16665 (C4X49_16655) pilE 3386599..3387060 (-) 462 WP_000993711.1 pilin Machinery gene

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 15590.71 Da        Isoelectric Point: 7.5868

>NTDB_id=281553 C4X49_RS16665 WP_000993711.1 3386599..3387060(-) (pilE) [Acinetobacter baumannii strain NCIMB 8209]
MNAQKGFTLIELMIVVAIIGILAAIAIPAYQDYIARSQVSEAMELASGLKGAVAETFSQDATCPTNGTNGIATATDINGK
YVASVTTAGTAAASGGCTITAKMRATGVSKGIQNGELTLTLSNADKGSNVWSCTSTGSNKIEQKYLPKSCTSS

Nucleotide


Download         Length: 462 bp        

>NTDB_id=281553 C4X49_RS16665 WP_000993711.1 3386599..3387060(-) (pilE) [Acinetobacter baumannii strain NCIMB 8209]
ATGAATGCACAAAAAGGTTTTACATTAATCGAACTCATGATCGTGGTTGCCATTATCGGTATTTTGGCAGCAATTGCGAT
TCCTGCGTATCAAGACTATATCGCTCGTTCTCAAGTTAGTGAAGCTATGGAGTTAGCTAGTGGATTGAAGGGAGCTGTTG
CAGAGACTTTTAGTCAAGATGCTACTTGTCCAACAAATGGTACTAATGGAATTGCTACTGCTACTGATATTAATGGTAAA
TATGTTGCTTCTGTAACCACGGCAGGTACCGCTGCTGCCTCAGGTGGATGTACAATTACTGCAAAAATGCGGGCAACTGG
AGTTTCAAAAGGTATTCAAAATGGAGAGCTTACTCTAACTCTTAGTAATGCAGATAAAGGTTCAAATGTGTGGAGTTGTA
CTTCAACTGGTTCAAACAAAATTGAACAAAAGTACTTACCTAAATCATGTACTTCTTCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A009I3S8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

47.826

100

0.503
  pilE Neisseria gonorrhoeae MS11

43.21

100

0.458
  pilA2 Legionella pneumophila strain ERS1305867

42.581

100

0.431
  pilA Acinetobacter baumannii strain A118

41.509

100

0.431
  pilA2 Legionella pneumophila str. Paris

43.046

98.693

0.425
  pilA Acinetobacter nosocomialis M2

52.459

79.739

0.418
  comP Acinetobacter baylyi ADP1

42.384

98.693

0.418
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.447

99.346

0.412
  pilA Ralstonia pseudosolanacearum GMI1000

39.13

100

0.412
  pilA Pseudomonas aeruginosa PAK

39.744

100

0.405
  pilA/pilA1 Eikenella corrodens VA1

38.217

100

0.392
  pilA Vibrio parahaemolyticus RIMD 2210633

41.791

87.582

0.366

Multiple sequence alignment