Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   C6570_RS01820 Genome accession   NZ_CP027666
Coordinates   400655..401791 (+) Length   378 a.a.
NCBI ID   WP_106701526.1    Uniprot ID   A0A2S0MBD3
Organism   Ottowia oryzae strain KADR8-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 395655..406791
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6570_RS01790 (C6570_01790) purU 396344..397192 (+) 849 WP_106701513.1 formyltetrahydrofolate deformylase -
  C6570_RS01795 (C6570_01795) - 397404..398000 (+) 597 WP_106701515.1 sigma-70 family RNA polymerase sigma factor -
  C6570_RS01800 (C6570_01800) - 398034..398402 (+) 369 WP_106701517.1 DUF5668 domain-containing protein -
  C6570_RS01805 (C6570_01805) - 398449..398670 (+) 222 WP_106701520.1 hypothetical protein -
  C6570_RS01810 (C6570_01810) - 398758..399453 (-) 696 WP_106701522.1 YggS family pyridoxal phosphate-dependent enzyme -
  C6570_RS01815 (C6570_01815) pilT 399512..400555 (+) 1044 WP_106701524.1 type IV pilus twitching motility protein PilT Machinery gene
  C6570_RS01820 (C6570_01820) pilU 400655..401791 (+) 1137 WP_106701526.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C6570_RS01825 (C6570_01825) - 401909..402826 (+) 918 WP_106704460.1 NAD(P)-dependent oxidoreductase -
  C6570_RS01830 (C6570_01830) - 403124..403906 (-) 783 WP_106701528.1 BON domain-containing protein -
  C6570_RS01835 (C6570_01835) - 403903..404502 (-) 600 WP_106701530.1 SIS domain-containing protein -
  C6570_RS01840 (C6570_01840) - 404613..404996 (-) 384 WP_106701532.1 YraN family protein -
  C6570_RS01845 (C6570_01845) rsmI 405030..405968 (+) 939 WP_106701534.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41871.14 Da        Isoelectric Point: 6.5908

>NTDB_id=278804 C6570_RS01820 WP_106701526.1 400655..401791(+) (pilU) [Ottowia oryzae strain KADR8-3]
MERDQASKFINDLLKLLLTRKGSDLFITADFPPAIKVDGKVTKVSAQNLTAAHTLALVRSVMNDKQAAEFERTKECNFAI
SPAGIGRFRCNAFVQQGNVGVVMRVIPQTLPTIDSLGMPQVLKDVAMSKRGLAIMVGATGSGKSTTLAAMVDWRNIHSQG
HIITVEDPVEFVHPHKNCIVTQREVGLDTDDWESALKNSLRQAPDVILMGEIRDRETMEHAVAFAETGHLCLATLHANSA
NQALDRIINFFPEERREQLLMDLSLNLRALVSQRLIAKQDGKGRTAVVEIMLNSPLISDLIFKGEVGEIKEVMKKSRELG
MQTFDQALFDAFEANLITYEDALRNADSANDLRLQIKLTSKRARSNDLAEGTEHLQIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=278804 C6570_RS01820 WP_106701526.1 400655..401791(+) (pilU) [Ottowia oryzae strain KADR8-3]
ATGGAACGCGATCAGGCCAGCAAGTTCATCAACGACCTGCTCAAGCTGCTGCTCACCCGCAAGGGCAGCGATCTGTTCAT
CACCGCCGACTTCCCGCCGGCCATCAAGGTCGACGGCAAGGTGACGAAGGTGTCTGCGCAGAACCTGACCGCGGCGCACA
CGCTGGCGCTGGTGCGCTCGGTGATGAACGACAAGCAGGCGGCCGAGTTCGAACGCACCAAGGAGTGCAACTTCGCCATT
TCTCCGGCGGGCATCGGCCGCTTTCGCTGCAACGCGTTCGTGCAGCAGGGCAACGTCGGCGTGGTGATGCGGGTGATCCC
GCAGACATTGCCCACGATCGATTCGCTGGGCATGCCTCAGGTGCTGAAGGACGTGGCCATGAGCAAGCGCGGCCTGGCCA
TCATGGTGGGCGCCACCGGTTCGGGTAAATCGACCACGCTGGCGGCCATGGTGGACTGGCGCAACATCCATTCGCAGGGC
CACATCATCACTGTGGAAGATCCGGTGGAGTTCGTGCACCCGCACAAGAACTGCATCGTCACCCAGCGCGAAGTGGGGCT
GGACACCGACGATTGGGAATCGGCCCTCAAGAACTCCCTGCGCCAGGCGCCCGACGTCATCCTGATGGGCGAAATCCGCG
ACCGCGAAACCATGGAACATGCCGTGGCTTTCGCAGAGACCGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAGCGCC
AACCAGGCACTCGACCGGATCATCAACTTCTTCCCCGAAGAGCGCCGCGAGCAGCTGCTGATGGACTTGTCGCTGAACCT
GCGCGCCCTGGTCTCGCAGCGCCTGATCGCCAAGCAGGATGGGAAGGGCCGCACCGCGGTGGTAGAGATTATGTTGAACT
CGCCGCTTATCTCGGATTTGATCTTCAAGGGCGAAGTGGGCGAGATCAAGGAAGTCATGAAGAAGAGCCGCGAGCTGGGC
ATGCAGACTTTCGACCAAGCGCTGTTCGATGCCTTCGAAGCCAACCTGATCACGTACGAAGACGCCCTGCGCAACGCCGA
TTCGGCCAACGACCTGCGCCTGCAGATCAAGCTGACCAGCAAGCGCGCCCGCTCGAACGATCTGGCGGAAGGCACGGAGC
ATCTGCAGATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(41-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S0MBD3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.178

92.328

0.574
  pilU Acinetobacter baylyi ADP1

56.941

93.386

0.532
  pilU Vibrio cholerae strain A1552

54.821

96.032

0.526
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.994

89.153

0.41
  pilT Pseudomonas aeruginosa PAK

43.323

89.153

0.386
  pilT Pseudomonas stutzeri DSM 10701

41.84

89.153

0.373
  pilT Legionella pneumophila strain Lp02

42.042

88.095

0.37
  pilT Legionella pneumophila strain ERS1305867

42.042

88.095

0.37
  pilT Acinetobacter nosocomialis M2

42.947

84.392

0.362
  pilT Acinetobacter baumannii D1279779

42.947

84.392

0.362
  pilT Acinetobacter baumannii strain A118

42.947

84.392

0.362

Multiple sequence alignment