Detailed information NTG0027101

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AZO_RS17425
• Genome accession: NC_008702
• Coordinates: 3805466..3806599 (+)
• Length: 377 a.a.
• NCBI ID: WP_011767191.1
• Uniprot ID: A1KB79
• Organism: Azoarcus olearius
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3800466..3811599

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AZO_RS17400 (azo3464)	-	3801700..3801996 (-)	297	WP_011767186.1	DUF167 domain-containing protein	-
AZO_RS17405 (azo3465)	-	3802001..3802582 (-)	582	WP_011767187.1	YggT family protein	-
AZO_RS17410 (azo3466)	proC	3802601..3803425 (-)	825	WP_011767188.1	pyrroline-5-carboxylate reductase	-
AZO_RS17415 (azo3467)	-	3803499..3804212 (-)	714	WP_011767189.1	YggS family pyridoxal phosphate-dependent enzyme	-
AZO_RS17420 (azo3468)	pilT	3804339..3805379 (+)	1041	WP_011767190.1	type IV pilus twitching motility protein PilT	Machinery gene
AZO_RS17425 (azo3469)	pilU	3805466..3806599 (+)	1134	WP_011767191.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AZO_RS17430 (azo3470)	-	3806788..3809505 (+)	2718	WP_011767192.1	type VI secretion system Vgr family protein	-

Sequence

Protein

• Length: 377 a.a.
• Molecular weight: 42065.48 Da
• Isoelectric Point: 6.3990

>NTDB_id=27101 AZO_RS17425 WP_011767191.1 3805466..3806599(+) (pilU) [Azoarcus olearius]
MERDQALKFMHDLLRLMLQKNGSDLFINPGFPPAIKVDGRIVPQSNQALAPQHTAELARAIMNDRQSAEFEATKECNFAI
SPAGIGRFRANAYIQQGRVALVLRTIAQKIPTIDELGLPPVLRDIGMTKRGLVIFVGGTGTGKTTSLAAMVDFRNENSYG
HIITIEDPIEYVHPHKNCIVSQREVGIDTDNWEAALKNTLRQAPDVILMGEIRDRETMDYAIAFAETGHLCLATLHANSA
NQAIDRIINFFPEDRRQQLLMDLSLNLRAMVSQRLLPLKGRKGRVPAVEVLLNSPLVSDLIFKGEVPGIKEIMKRSRELG
MQTFDQALFDLYEAELISYEDALRNADSVNDLRLQIKLNSKLGDKDLVSGIQNLDIV

Nucleotide

• Length: 1134 bp

>NTDB_id=27101 AZO_RS17425 WP_011767191.1 3805466..3806599(+) (pilU) [Azoarcus olearius]
ATGGAACGCGACCAGGCCCTGAAATTCATGCACGACCTGCTGCGGCTGATGCTGCAGAAGAACGGTTCCGACCTCTTCAT
CAATCCCGGCTTTCCGCCGGCGATCAAGGTCGACGGACGCATCGTGCCGCAGTCCAACCAGGCGCTGGCGCCGCAGCACA
CCGCGGAACTTGCGCGCGCGATCATGAACGACCGCCAGTCGGCCGAATTCGAAGCCACCAAGGAGTGCAACTTCGCGATC
TCGCCCGCGGGCATCGGCCGCTTCCGGGCCAATGCCTACATCCAGCAAGGCCGCGTGGCGCTGGTGTTGCGCACGATCGC
GCAGAAGATCCCGACCATCGACGAACTCGGCCTGCCGCCGGTATTGCGCGACATCGGCATGACCAAGCGCGGCCTGGTGA
TCTTCGTCGGCGGCACCGGCACCGGCAAGACCACCTCGCTGGCGGCGATGGTCGACTTCCGCAATGAGAACAGCTACGGC
CACATCATCACGATCGAAGATCCGATCGAGTACGTGCACCCGCACAAGAACTGCATCGTGTCGCAACGCGAAGTCGGCAT
CGACACCGACAACTGGGAAGCGGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATGGGCGAAATCCGTG
ACCGCGAAACCATGGATTACGCGATCGCCTTCGCCGAAACCGGCCACCTCTGCCTTGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGATCGACCGCATCATCAACTTCTTCCCGGAAGACCGCCGCCAGCAACTGCTGATGGATTTGTCGCTGAACCT
GCGGGCGATGGTTTCGCAGCGCCTGCTGCCGCTCAAGGGGCGCAAGGGCCGCGTGCCCGCGGTCGAGGTGCTGCTCAACT
CCCCGCTGGTGTCGGACCTGATCTTCAAGGGCGAGGTGCCAGGCATCAAGGAGATCATGAAGCGCTCCCGCGAACTGGGC
ATGCAGACCTTCGACCAGGCCTTGTTCGACCTCTACGAAGCCGAGCTGATCAGCTACGAAGACGCGTTGCGCAATGCCGA
TTCGGTGAATGACCTGCGGCTGCAGATCAAGCTCAACAGCAAGCTGGGCGACAAGGACCTCGTCTCGGGTATCCAGAACC
TCGACATCGTCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (125-275)

3D structure

• AlphaFold DB: A1KB79

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	62.363	96.552	0.602
pilU	Acinetobacter baylyi ADP1	58.974	93.103	0.549
pilU	Vibrio cholerae strain A1552	53.056	95.491	0.507
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.017	94.96	0.408
pilT	Pseudomonas stutzeri DSM 10701	41.791	88.859	0.371
pilT	Acinetobacter baylyi ADP1	40.95	89.39	0.366
pilT	Legionella pneumophila strain Lp02	41.692	87.798	0.366
pilT	Legionella pneumophila strain ERS1305867	41.692	87.798	0.366
pilT	Pseudomonas aeruginosa PAK	41.194	88.859	0.366
pilT	Acinetobacter nosocomialis M2	41.088	87.798	0.361
pilT	Acinetobacter baumannii D1279779	41.088	87.798	0.361
pilT	Acinetobacter baumannii strain A118	41.088	87.798	0.361