Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   SmaCSM2_RS16935 Genome accession   NZ_CP025298
Coordinates   3683036..3683485 (-) Length   149 a.a.
NCBI ID   WP_049442650.1    Uniprot ID   A0AAD0FNJ2
Organism   Stenotrophomonas maltophilia strain CSM2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3683840..3696765 3683036..3683485 flank 355


Gene organization within MGE regions


Location: 3683036..3696765
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SmaCSM2_RS16935 (SmaCSM2_16935) pilA 3683036..3683485 (-) 450 WP_049442650.1 pilin Machinery gene
  SmaCSM2_RS16940 (SmaCSM2_16940) pilC 3683840..3685099 (+) 1260 WP_049442651.1 type II secretion system F family protein Machinery gene
  SmaCSM2_RS16945 (SmaCSM2_16945) - 3685108..3685971 (+) 864 WP_005418626.1 A24 family peptidase -
  SmaCSM2_RS16950 (SmaCSM2_16950) coaE 3685983..3686594 (+) 612 WP_101765984.1 dephospho-CoA kinase -
  SmaCSM2_RS16955 (SmaCSM2_16955) - 3686655..3687041 (-) 387 WP_101765985.1 hypothetical protein -
  SmaCSM2_RS16960 (SmaCSM2_16960) - 3687526..3688896 (-) 1371 WP_075676409.1 HAMP domain-containing sensor histidine kinase -
  SmaCSM2_RS16965 (SmaCSM2_16965) - 3688859..3689536 (-) 678 WP_005410804.1 response regulator transcription factor -
  SmaCSM2_RS16970 (SmaCSM2_16970) - 3689700..3690161 (-) 462 WP_049442760.1 hypothetical protein -
  SmaCSM2_RS16975 (SmaCSM2_16975) - 3690277..3690747 (-) 471 WP_049442672.1 hypothetical protein -
  SmaCSM2_RS16980 (SmaCSM2_16980) - 3690843..3691322 (-) 480 WP_049442673.1 hypothetical protein -
  SmaCSM2_RS16985 (SmaCSM2_16985) rimK 3691581..3692459 (-) 879 WP_005418651.1 30S ribosomal protein S6--L-glutamate ligase -
  SmaCSM2_RS16990 (SmaCSM2_16990) - 3692721..3693161 (-) 441 WP_158298007.1 calcium-binding protein -
  SmaCSM2_RS22190 (SmaCSM2_16995) - 3693217..3696765 (-) 3549 WP_101765987.1 calcium-binding protein -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 14968.28 Da        Isoelectric Point: 8.4743

>NTDB_id=259894 SmaCSM2_RS16935 WP_049442650.1 3683036..3683485(-) (pilA) [Stenotrophomonas maltophilia strain CSM2]
MKNQKGFTLIELMIVVAIIAILAAIALPAYQDYVVKSRVSEAMVLADGLKAVVADNAAQGESDLGKGATLTKAADGSPNV
TSTAIDATSGAITVTTTSKAGNGDVILTPKAGGAALAAGTPPEGTIVWTCTSTKIKQKYLPATCTSSAK

Nucleotide


Download         Length: 450 bp        

>NTDB_id=259894 SmaCSM2_RS16935 WP_049442650.1 3683036..3683485(-) (pilA) [Stenotrophomonas maltophilia strain CSM2]
ATGAAGAACCAGAAGGGCTTCACCCTTATCGAACTGATGATCGTCGTTGCGATCATCGCCATCCTGGCCGCCATTGCTCT
GCCGGCCTATCAGGATTATGTCGTCAAGTCGCGCGTTTCTGAAGCGATGGTGCTGGCCGATGGCCTGAAGGCTGTCGTTG
CTGACAATGCCGCGCAGGGCGAATCTGATCTGGGCAAGGGTGCCACTCTTACCAAGGCTGCTGACGGTTCGCCGAACGTG
ACGTCGACCGCAATTGATGCGACTTCGGGCGCGATTACTGTTACCACGACCAGCAAGGCTGGTAATGGCGACGTCATCCT
GACTCCGAAGGCTGGTGGCGCAGCACTGGCCGCTGGTACCCCGCCGGAGGGCACCATCGTCTGGACCTGCACCTCTACCA
AGATCAAGCAGAAGTACCTGCCGGCTACCTGCACCAGCTCGGCCAAGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-145)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

45.57

100

0.483
  pilA2 Legionella pneumophila strain ERS1305867

48.252

95.973

0.463
  pilA2 Legionella pneumophila str. Paris

47.552

95.973

0.456
  comP Acinetobacter baylyi ADP1

41.139

100

0.436
  pilA/pilAI Pseudomonas stutzeri DSM 10701

42

100

0.423
  pilA Pseudomonas aeruginosa PAK

39.744

100

0.416
  pilE Neisseria gonorrhoeae MS11

34.911

100

0.396
  pilA Vibrio cholerae strain A1552

34.568

100

0.376
  pilA Vibrio cholerae C6706

34.568

100

0.376
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

34.568

100

0.376
  pilA Acinetobacter baumannii strain A118

37.162

99.329

0.369

Multiple sequence alignment