Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PSEEN_RS01520 Genome accession   NC_008027
Coordinates   344543..345814 (+) Length   423 a.a.
NCBI ID   WP_373694282.1    Uniprot ID   -
Organism   Pseudomonas entomophila L48     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 339543..350814
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSEEN_RS01500 (PSEEN0328) - 339578..342028 (-) 2451 WP_373694319.1 penicillin-binding protein 1A -
  PSEEN_RS01505 (PSEEN0330) pilM 342201..343076 (+) 876 WP_011531750.1 type IV pilus biogenesis protein PilM -
  PSEEN_RS01510 (PSEEN0331) - 343073..343612 (+) 540 WP_011531751.1 PilN domain-containing protein -
  PSEEN_RS01515 (PSEEN0332) - 343660..344610 (+) 951 WP_086009434.1 pilus assembly protein PilP -
  PSEEN_RS01520 (PSEEN0333) pilQ 344543..345814 (+) 1272 WP_373694282.1 type IV pilus secretin PilQ Machinery gene
  PSEEN_RS01525 (PSEEN0334) aroK 345819..346337 (+) 519 WP_011531754.1 shikimate kinase AroK -
  PSEEN_RS01530 (PSEEN0335) aroB 346435..347532 (+) 1098 WP_011531755.1 3-dehydroquinate synthase -
  PSEEN_RS01535 (PSEEN0336) - 347548..349167 (+) 1620 WP_011531756.1 AAA family ATPase -

Sequence

Protein


Download         Length: 423 a.a.        Molecular weight: 46016.80 Da        Isoelectric Point: 4.8746

>NTDB_id=25899 PSEEN_RS01520 WP_373694282.1 344543..345814(+) (pilQ) [Pseudomonas entomophila L48]
MRLTGLASAMLVLSGVRAALAAEFQGEPMSLNFQDVEVRGVLQVMADYAGINLVASDAVQGRITLRLEEVPWDQALDLVL
RSKGLGRRQEGNVLLIAPIAELAEQSRGAHLEQALDARLEPLRRELIPVYHAKASDLAELLLASLADDGILAGRGSLSVD
ARTNTLVVAQPADRLGEIRRLVAQLDVPVRQVMIEARIVEANIDYEKALGVRWGGPLYGQDNRLGSELFVDLGVERATSA
LGVGLLRSDVLLDLELSAMEKSGNGEIISQPRVVTADKETARILKGTEVPYQETTKSGATSISFREASLSLEVTPQITPD
GKVIMEVRVTKDEPDFVNALNNVPPIRKNEVNAKVRVTNGETIVIGGVYSTSQNNVVDKVPFFGDLPYVGRLFRRDALQE
KKSELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1272 bp        

>NTDB_id=25899 PSEEN_RS01520 WP_373694282.1 344543..345814(+) (pilQ) [Pseudomonas entomophila L48]
ATGAGATTGACGGGGCTGGCGAGCGCGATGCTGGTGCTGTCGGGCGTGCGGGCAGCGCTGGCGGCTGAGTTTCAGGGTGA
ACCGATGTCGTTGAACTTCCAGGATGTGGAAGTGCGTGGGGTGTTGCAGGTCATGGCCGACTACGCCGGTATCAACCTGG
TGGCCAGCGATGCCGTCCAGGGGCGGATCACCCTCAGGCTGGAGGAGGTGCCTTGGGATCAGGCGCTCGACCTTGTGTTA
CGCAGCAAGGGGTTGGGGCGCCGGCAGGAGGGGAATGTACTGTTGATCGCGCCCATCGCCGAACTCGCCGAGCAGTCGCG
CGGTGCTCACCTGGAGCAGGCGCTGGACGCTCGACTTGAGCCGTTGCGGCGGGAGCTGATACCGGTCTATCACGCCAAGG
CGTCGGACCTGGCCGAATTGCTGCTGGCGAGCCTGGCTGACGACGGCATCCTGGCCGGGCGCGGTAGCCTGAGCGTGGAT
GCACGCACCAATACGCTGGTGGTTGCCCAGCCCGCTGATCGGCTGGGTGAAATCCGCCGGCTGGTGGCCCAGCTCGATGT
GCCAGTGCGCCAGGTCATGATCGAGGCTCGCATTGTCGAGGCCAACATTGATTATGAAAAGGCCTTGGGCGTGCGCTGGG
GTGGCCCGCTGTATGGCCAGGACAACCGTCTGGGCAGCGAGCTGTTCGTCGACCTGGGTGTGGAACGAGCGACCTCGGCG
CTTGGTGTCGGGTTGTTGCGCAGTGACGTCCTGCTCGACCTGGAGCTCAGCGCGATGGAGAAAAGCGGTAATGGCGAGAT
CATCTCCCAGCCCAGGGTCGTCACCGCCGACAAGGAGACGGCGCGCATTCTCAAGGGTACCGAGGTCCCCTACCAGGAGA
CTACCAAGAGCGGTGCCACCTCCATCTCGTTTCGCGAAGCCTCCCTGTCGCTGGAGGTGACACCGCAGATCACCCCCGAT
GGCAAGGTGATCATGGAGGTGCGGGTGACCAAGGACGAGCCGGACTTTGTCAACGCCCTGAACAACGTGCCCCCCATCCG
CAAGAACGAAGTCAACGCCAAGGTGCGGGTCACCAACGGAGAAACCATTGTGATTGGTGGGGTGTACTCGACGTCGCAGA
ACAATGTGGTCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAG
AAAAAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(258-414)

Secretin and TonB N terminus short domain (STN)

(52-98)

Bacterial type II/III secretion system short domain (Secretin_N)

(126-191)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

58.486

100

0.603
  comQ Acinetobacter baylyi ADP1

44.222

100

0.47
  pilQ Acinetobacter baumannii strain A118

42.919

100

0.466
  pilQ Acinetobacter baumannii D1279779

42.919

100

0.466
  pilQ Legionella pneumophila strain ERS1305867

43.78

98.818

0.433
  pilQ Legionella pneumophila strain Lp02

43.78

98.818

0.433
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

43.062

98.818

0.426
  pilQ Vibrio cholerae strain A1552

43.062

98.818

0.426
  pilQ Vibrio campbellii strain DS40M4

42.217

100

0.423
  pilQ Neisseria gonorrhoeae strain FA1090

37.47

99.054

0.371
  pilQ Neisseria meningitidis M1080

37.232

99.054

0.369
  pilQ Neisseria meningitidis MC58

36.603

98.818

0.362

Multiple sequence alignment