Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CS557_RS04350 Genome accession   NZ_CP024632
Coordinates   904440..905558 (-) Length   372 a.a.
NCBI ID   WP_004913902.1    Uniprot ID   S7Y7Y7
Organism   Acinetobacter junii strain lzh-X15     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 899440..910558
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CS557_RS04320 (CS557_04330) - 900113..900961 (+) 849 WP_005403076.1 class II glutamine amidotransferase -
  CS557_RS04325 (CS557_04335) - 901244..901699 (+) 456 WP_004913914.1 bacteriohemerythrin -
  CS557_RS04330 (CS557_04340) - 901938..903017 (+) 1080 WP_099948240.1 hypothetical protein -
  CS557_RS04335 (CS557_04345) - 903017..903340 (+) 324 WP_099948241.1 RnfH family protein -
  CS557_RS04340 (CS557_04350) - 903396..903794 (-) 399 WP_004913908.1 outer membrane protein assembly factor BamE -
  CS557_RS04345 (CS557_04355) fur 903906..904343 (+) 438 WP_004952725.1 ferric iron uptake transcriptional regulator -
  CS557_RS04350 (CS557_04360) pilU 904440..905558 (-) 1119 WP_004913902.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CS557_RS04355 (CS557_04365) pilT 905584..906621 (-) 1038 WP_004960020.1 type IV pilus twitching motility protein PilT Machinery gene
  CS557_RS04360 (CS557_04370) - 906749..907435 (+) 687 WP_099948242.1 YggS family pyridoxal phosphate-dependent enzyme -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41716.88 Da        Isoelectric Point: 6.1368

>NTDB_id=254585 CS557_RS04350 WP_004913902.1 904440..905558(-) (pilU) [Acinetobacter junii strain lzh-X15]
MDFNDLLNLMIEQSASDLFVTADVEPSMKINGQIVPVAKAKLSGEIVGQLLHSIMSDKQRKEFEDTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETVIPTMDELKLPAILKDLAMTKRGIIIFVGATGTGKSTSLASMIGYRNQNSKGHIITIE
DPIEFVHQHAGCIVTQREVGIDTDSFEVALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVLATLHANNANQALDR
IIHFFEADRHAQLHMDLSLNLRAMVAQQLIPTPDGNSRRAAIEILINTPLLSDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALYDLYKAGEITYKDALKHADSPNDLRLTIKLSEEGADQLLSASRNITFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=254585 CS557_RS04350 WP_004913902.1 904440..905558(-) (pilU) [Acinetobacter junii strain lzh-X15]
ATGGATTTTAATGATTTACTCAATCTAATGATTGAACAAAGTGCTTCGGATTTATTTGTTACCGCTGATGTTGAACCTTC
AATGAAAATCAACGGTCAGATTGTTCCTGTGGCTAAAGCAAAACTTTCAGGCGAGATTGTAGGTCAGTTATTACACTCAA
TCATGAGTGATAAACAACGTAAAGAATTCGAAGATACTCGCGAGTGTAACTTCGCAATTATGAACCGTGAGAAAACAGCG
CGTTTTCGTGTGAGCGCGTTCCAACAACGTGATATGCCAGGCATGGTATTACGTCGTATCGAAACGGTTATTCCAACGAT
GGATGAATTAAAACTACCTGCAATCCTCAAAGATTTAGCGATGACCAAACGTGGCATAATTATCTTTGTTGGTGCAACAG
GTACAGGTAAATCAACCTCGCTCGCGTCAATGATCGGATATCGTAACCAAAACTCTAAAGGTCATATCATCACCATTGAA
GACCCGATCGAGTTTGTACACCAACACGCGGGTTGTATTGTGACTCAACGAGAAGTAGGTATTGATACAGACTCTTTTGA
AGTAGCTTTGAAAAATACACTTCGTCAAGCACCCGATGTGATCTTGATTGGTGAGATTCGCTCTCGTGAAACAATGGACT
ATGCGATTGCTTTCGCAGAAACAGGTCACTTAGTACTTGCTACACTTCACGCGAATAACGCTAACCAAGCATTAGACCGT
ATTATTCACTTCTTTGAAGCTGACCGTCATGCTCAACTACATATGGATTTATCGCTCAATCTAAGAGCTATGGTTGCTCA
ACAGTTAATTCCAACACCTGATGGAAATTCGCGCCGAGCAGCTATCGAAATTCTAATTAATACACCGTTACTATCAGATT
ATATCCGTAAAGGTGAAATTCATGAGATTAAAGACCTCATGAAACGCTCGCGCGAATTGGGCATGCAAACATTCGACCAA
GCGCTTTATGATTTATATAAAGCTGGTGAAATCACATATAAAGATGCGCTCAAACATGCAGACTCACCAAACGATTTACG
TCTCACCATTAAACTTTCTGAAGAAGGTGCAGATCAATTGTTAAGTGCAAGTCGCAACATTACATTTGATGGTCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S7Y7Y7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.132

100

0.763
  pilU Pseudomonas stutzeri DSM 10701

70.62

99.731

0.704
  pilU Vibrio cholerae strain A1552

55.135

99.462

0.548
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.486

93.011

0.395
  pilT Pseudomonas aeruginosa PAK

39.884

93.011

0.371
  pilT Neisseria gonorrhoeae MS11

40.058

91.935

0.368
  pilT Neisseria meningitidis 8013

40.058

91.935

0.368
  pilT Acinetobacter nosocomialis M2

39.65

92.204

0.366
  pilT Acinetobacter baumannii D1279779

39.65

92.204

0.366
  pilT Acinetobacter baumannii strain A118

39.65

92.204

0.366
  pilT Legionella pneumophila strain Lp02

40.476

90.323

0.366
  pilT Legionella pneumophila strain ERS1305867

40.476

90.323

0.366

Multiple sequence alignment