Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   CSH50_RS24670 Genome accession   NZ_CP024159
Coordinates   5352687..5353892 (-) Length   401 a.a.
NCBI ID   WP_096050272.1    Uniprot ID   -
Organism   Pseudomonas mosselii strain PtA1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5347687..5358892
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CSH50_RS24655 - 5349336..5350955 (-) 1620 WP_096049176.1 AAA family ATPase -
  CSH50_RS24660 aroB 5350971..5352068 (-) 1098 WP_096049175.1 3-dehydroquinate synthase -
  CSH50_RS24665 aroK 5352164..5352682 (-) 519 WP_028689109.1 shikimate kinase AroK -
  CSH50_RS24670 pilQ 5352687..5353892 (-) 1206 WP_096050272.1 type IV pilus secretin PilQ Machinery gene
  CSH50_RS24675 - 5353849..5354862 (-) 1014 WP_096049174.1 pilus assembly protein PilP -
  CSH50_RS24680 - 5354859..5355398 (-) 540 WP_062575467.1 PilN domain-containing protein -
  CSH50_RS24685 pilM 5355395..5356291 (-) 897 WP_096049173.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 401 a.a.        Molecular weight: 43424.88 Da        Isoelectric Point: 4.8325

>NTDB_id=252586 CSH50_RS24670 WP_096050272.1 5352687..5353892(-) (pilQ) [Pseudomonas mosselii strain PtA1]
MLGLQMTQAETFQGEPMSLNLQDVELRSVLQVMADHAGVNLVVGDSVQGRITLRLEDVPWEQALDLVLRSKGLGRRQEGN
VLLVAPVAELAGQVQGIEPLQRELIPVFHAKAGELAELLLASLADDGAVAGRGSLSVDARTNTLVATQPAVRLAELRRLV
TQLDVPVRQVMIEARIVEANVDYEKALGVRWGGPLYGSDPRLGSDLFVDLGVERGGAALGVGLLRSDILLDLELSAMEKS
GNGEIISQPKVVTADKETARILKGTEVPYQETTRSGATSVSFREASLSLEVTPQITPDGKVIMAVRVTKDEPDFINALND
VPPIRKNEVNAKVRVTDGQTIVIGGVYSTSQNKVVDKVPFFGDLPYVGRLFRRDALQEKKSELLVFLTPRIMSDQAIAVS
R

Nucleotide


Download         Length: 1206 bp        

>NTDB_id=252586 CSH50_RS24670 WP_096050272.1 5352687..5353892(-) (pilQ) [Pseudomonas mosselii strain PtA1]
ATGCTGGGGCTTCAGATGACGCAGGCCGAGACGTTTCAGGGTGAGCCGATGTCGCTCAACCTCCAGGACGTCGAGCTGCG
TTCGGTGCTTCAGGTCATGGCCGATCACGCGGGTGTCAACCTGGTGGTCGGCGACTCGGTGCAGGGGCGGATTACCCTGC
GCCTGGAGGACGTGCCCTGGGAGCAGGCGCTTGATCTGGTGCTGCGCAGCAAGGGCCTGGGCAGGCGCCAGGAGGGGAAT
GTGCTGCTGGTTGCTCCTGTGGCAGAGCTCGCTGGGCAGGTCCAGGGCATCGAGCCACTGCAGCGTGAATTGATCCCTGT
CTTCCATGCCAAGGCCGGCGAGCTGGCGGAGCTGCTGCTGGCGAGCCTGGCGGACGACGGTGCCGTGGCCGGGCGTGGCA
GCCTCAGTGTGGATGCTCGCACCAATACCCTGGTAGCCACCCAGCCTGCCGTGCGGCTCGCCGAACTGCGCAGGCTGGTC
ACGCAGCTGGATGTGCCGGTGCGCCAGGTCATGATCGAGGCACGGATCGTCGAGGCCAATGTCGACTACGAAAAGGCACT
GGGCGTGCGCTGGGGTGGACCGCTGTATGGCTCGGACCCCCGGCTGGGCAGCGACCTTTTCGTCGATCTTGGCGTCGAGC
GGGGAGGTGCGGCGTTGGGGGTGGGGCTGCTGCGCAGCGATATCCTGCTCGACCTGGAGCTCAGCGCCATGGAAAAAAGC
GGCAATGGCGAGATCATCTCCCAGCCCAAGGTGGTTACGGCCGACAAGGAAACCGCGCGCATACTCAAGGGTACCGAAGT
GCCCTACCAGGAAACCACCCGCAGTGGCGCCACTTCGGTCTCCTTCCGCGAGGCGTCCCTGTCGCTGGAAGTGACGCCGC
AGATCACGCCGGACGGCAAGGTGATCATGGCGGTGCGGGTGACCAAGGACGAGCCGGACTTCATCAATGCCCTCAACGAT
GTGCCGCCGATCCGCAAGAACGAGGTCAACGCCAAGGTGCGGGTGACCGATGGGCAGACCATTGTGATTGGCGGCGTGTA
CTCGACGTCGCAGAACAAAGTGGTCGACAAGGTGCCATTTTTTGGTGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCG
ATGCATTACAAGAGAAAAAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGT
CGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(104-169)

Secretin and TonB N terminus short domain (STN)

(39-86)

Bacterial type II and III secretion system protein (Secretin)

(236-392)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

57.11

100

0.621
  comQ Acinetobacter baylyi ADP1

42.163

100

0.476
  pilQ Acinetobacter baumannii D1279779

40.086

100

0.464
  pilQ Acinetobacter baumannii strain A118

40.086

100

0.464
  pilQ Legionella pneumophila strain ERS1305867

42.654

100

0.449
  pilQ Legionella pneumophila strain Lp02

42.654

100

0.449
  pilQ Vibrio campbellii strain DS40M4

39.858

100

0.421
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

39.904

100

0.414
  pilQ Vibrio cholerae strain A1552

39.904

100

0.414
  pilQ Neisseria meningitidis M1080

35.934

100

0.379
  pilQ Neisseria gonorrhoeae strain FA1090

35.545

100

0.374
  pilQ Neisseria meningitidis MC58

35.071

100

0.369

Multiple sequence alignment