Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   BBX44_RS16960 Genome accession   NZ_CP024024
Coordinates   3598237..3598686 (-) Length   149 a.a.
NCBI ID   WP_003112842.1    Uniprot ID   P04739
Organism   Pseudomonas aeruginosa strain PARM801     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3582013..3601842 3598237..3598686 within 0


Gene organization within MGE regions


Location: 3582013..3601842
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BBX44_RS16830 - 3582013..3582729 (-) 717 WP_073624880.1 hypothetical protein -
  BBX44_RS16835 - 3582878..3583078 (+) 201 WP_023435053.1 helix-turn-helix domain-containing protein -
  BBX44_RS16840 - 3583075..3583491 (+) 417 WP_023435054.1 hypothetical protein -
  BBX44_RS16845 - 3583484..3583717 (+) 234 WP_023110507.1 hypothetical protein -
  BBX44_RS16850 - 3583714..3583962 (+) 249 WP_073653397.1 hypothetical protein -
  BBX44_RS16855 - 3583959..3584264 (+) 306 WP_073653396.1 hypothetical protein -
  BBX44_RS33860 - 3584267..3584704 (+) 438 WP_343229373.1 hypothetical protein -
  BBX44_RS16865 - 3584743..3585210 (+) 468 WP_031629313.1 hypothetical protein -
  BBX44_RS16870 - 3585217..3586170 (+) 954 WP_023107347.1 DUF3631 domain-containing protein -
  BBX44_RS16875 - 3586170..3586463 (+) 294 WP_023107348.1 hypothetical protein -
  BBX44_RS16880 - 3586519..3587415 (+) 897 WP_023107349.1 hypothetical protein -
  BBX44_RS16885 - 3587546..3587959 (+) 414 WP_031629315.1 hypothetical protein -
  BBX44_RS16890 - 3588110..3588685 (-) 576 WP_124715997.1 hypothetical protein -
  BBX44_RS16895 - 3589422..3589928 (-) 507 WP_003096122.1 GNAT family N-acetyltransferase -
  BBX44_RS16900 - 3590689..3590970 (+) 282 WP_033978094.1 hypothetical protein -
  BBX44_RS16905 - 3590970..3591206 (+) 237 WP_003096116.1 hypothetical protein -
  BBX44_RS16910 - 3591413..3591694 (-) 282 WP_023435065.1 hypothetical protein -
  BBX44_RS16915 - 3591891..3592589 (+) 699 WP_124014670.1 hypothetical protein -
  BBX44_RS16920 - 3592614..3593618 (+) 1005 WP_031689993.1 pesticin C-terminus-like muramidase -
  BBX44_RS16925 - 3593631..3594293 (+) 663 WP_033992423.1 DUF3218 family protein -
  BBX44_RS16930 fliP 3594573..3595328 (+) 756 WP_031285507.1 flagellar type III secretion system pore protein FliP -
  BBX44_RS16935 fliQ 3595373..3595642 (+) 270 WP_023435365.1 flagellar biosynthesis protein FliQ -
  BBX44_RS16940 fliR 3595642..3596424 (+) 783 Protein_3366 flagellar biosynthetic protein FliR -
  BBX44_RS16945 - 3596427..3596686 (+) 260 Protein_3367 EscU/YscU/HrcU family type III secretion system export apparatus switch protein -
  BBX44_RS16950 - 3597715..3597957 (+) 243 WP_023082027.1 hypothetical protein -
  BBX44_RS16960 pilA 3598237..3598686 (-) 450 WP_003112842.1 type 4a pilus biogenesis protein PilA Machinery gene
  BBX44_RS16965 pilB 3598918..3600618 (+) 1701 WP_003112841.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BBX44_RS16970 pilC 3600622..3601842 (+) 1221 WP_003112840.1 type 4a pilus biogenesis protein PilC Machinery gene

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15511.78 Da        Isoelectric Point: 6.2905

>NTDB_id=251238 BBX44_RS16960 WP_003112842.1 3598237..3598686(-) (pilA) [Pseudomonas aeruginosa strain PARM801]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKIKIGTTASTATETYV
GVEPDANKLGVIAVAIEDSGAGDITFTFQTGTSSPKNATKVITLNRTADGVWACKSTQDPMFTPKGCDN

Nucleotide


Download         Length: 450 bp        

>NTDB_id=251238 BBX44_RS16960 WP_003112842.1 3598237..3598686(-) (pilA) [Pseudomonas aeruginosa strain PARM801]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCAATTGCCAT
TCCCCAGTATCAGAACTATGTTGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAACCCGCTGAAGACCACTGTTG
AAGAGTCGCTGTCGCGTGGAATTGCTGGTAGCAAAATTAAAATTGGTACTACTGCTTCTACTGCGACCGAAACATATGTC
GGCGTCGAGCCGGATGCCAACAAGTTGGGTGTAATTGCTGTAGCAATCGAAGATAGTGGTGCGGGTGATATTACCTTTAC
CTTCCAGACTGGTACCTCTAGTCCCAAGAATGCTACTAAAGTTATCACTCTGAACCGTACTGCGGATGGGGTCTGGGCTT
GTAAATCTACCCAGGATCCGATGTTCACTCCGAAAGGTTGTGATAACTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-147)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 1PAN
  PDB 1PAO
  PDB 8TUM
  PDB 8TUW
  PDB 9EWX

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

67.785

100

0.678
  pilA Acinetobacter baumannii strain A118

48

100

0.483
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

48.322

100

0.483
  pilA Vibrio cholerae strain A1552

48.322

100

0.483
  pilA Vibrio cholerae C6706

48.322

100

0.483
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.638

100

0.456
  pilA Vibrio parahaemolyticus RIMD 2210633

44.898

98.658

0.443
  comP Acinetobacter baylyi ADP1

41.83

100

0.43
  pilA/pilAII Pseudomonas stutzeri DSM 10701

44.056

95.973

0.423
  pilA2 Legionella pneumophila str. Paris

38.776

98.658

0.383
  pilA2 Legionella pneumophila strain ERS1305867

38.095

98.658

0.376

Multiple sequence alignment